Rice Science ›› 2023, Vol. 30 ›› Issue (5): 449-458.DOI: 10.1016/j.rsci.2023.03.016
收稿日期:
2022-11-10
接受日期:
2023-03-08
出版日期:
2023-09-28
发布日期:
2023-08-14
. [J]. Rice Science, 2023, 30(5): 449-458.
Condition | Root length (RL, mm) | Coleoptile length (CL, mm) | Ratio of RL to CL | H2O2 concentration (µmol/g) | TBARS concentration (nmol/g) | Phenol concentration (mg/g) |
---|---|---|---|---|---|---|
Water | 76.49 ± 3.03 a | 23.71 ± 0.67 bc | 3.22 ± 0.12 a | 0.50 ± 0.02 b | 12.06 ± 0.14 b | 0.35 ± 0.00 b |
NP10 | 85.58 ± 2.59 a | 27.28 ± 0.75 a | 3.14 ± 0.22 a | 0.47 ± 0.07 b | 12.84 ± 0.07 a | 0.31 ± 0.00 d |
NP100 | 62.80 ± 1.84 b | 25.64 ± 0.76 b | 2.45 ± 0.04 b | 0.72 ± 0.07 a | 10.41 ± 0.14 c | 0.44 ± 0.01 a |
B10 | 76.27 ± 2.62 a | 21.25 ± 0.75 c | 3.59 ± 0.24 a | 0.49 ± 0.07 b | 9.64 ± 0.07 d | 0.26 ± 0.00 e |
B100 | 54.25 ± 1.99 b | 22.35 ± 0.68 c | 2.43 ± 0.03 b | 0.61 ± 0.02 ab | 10.28 ± 0.14 c | 0.33 ± 0.00 c |
Table 1. Lengths of root and coleoptile, and concentrations of hydrogen peroxide, thiobarbituric acid reactive substance (TBARS) and phenol in rice after 7 d of imbibition under different conditions.
Condition | Root length (RL, mm) | Coleoptile length (CL, mm) | Ratio of RL to CL | H2O2 concentration (µmol/g) | TBARS concentration (nmol/g) | Phenol concentration (mg/g) |
---|---|---|---|---|---|---|
Water | 76.49 ± 3.03 a | 23.71 ± 0.67 bc | 3.22 ± 0.12 a | 0.50 ± 0.02 b | 12.06 ± 0.14 b | 0.35 ± 0.00 b |
NP10 | 85.58 ± 2.59 a | 27.28 ± 0.75 a | 3.14 ± 0.22 a | 0.47 ± 0.07 b | 12.84 ± 0.07 a | 0.31 ± 0.00 d |
NP100 | 62.80 ± 1.84 b | 25.64 ± 0.76 b | 2.45 ± 0.04 b | 0.72 ± 0.07 a | 10.41 ± 0.14 c | 0.44 ± 0.01 a |
B10 | 76.27 ± 2.62 a | 21.25 ± 0.75 c | 3.59 ± 0.24 a | 0.49 ± 0.07 b | 9.64 ± 0.07 d | 0.26 ± 0.00 e |
B100 | 54.25 ± 1.99 b | 22.35 ± 0.68 c | 2.43 ± 0.03 b | 0.61 ± 0.02 ab | 10.28 ± 0.14 c | 0.33 ± 0.00 c |
Fig. 1. Lateral root (LR) density (A, expressed as number of LRs/cm) and indole-3-acetic acid concentration (IAA, B) in roots of rice after 7 d of imbibition under different conditions. CK, Control (water); NP10, 10 mg/L zinc oxide nanoparticle (NP-ZnO); NP100, 100 mg/L NP-ZnO; B10, 10 mg/L bulk counterpart (B-ZnO); B100, 100 mg/L B-ZnO. Data are Mean ± SE (n = 4). Different lowercase letters above the bars indicate significant differences by the post hoc Tukey test (P ≤ 0.05).
Fig. 2. Histochemical detection of Zn in rice roots of comparable developmental stage after treatment with dithizone. A and B, Control (water); C and D, 10 mg/L zinc oxide nanoparticle (NP-ZnO); E and F, 100 mg/L NP-ZnO; G and H, 10 mg/L bulk counterpart (B-ZnO); I and J, 100 mg/L B-ZnO. The images on the right side show representative details at higher magnification of the roots on the left side.
Fig. 3. Histochemical detection of H2O2 by amplex ultra-red reagent (A) and lipid peroxidation by bodipy reagent (B). CK, Control (water); NP10, 10 mg/L zinc oxide nanoparticle (NP-ZnO); NP100, 100 mg/L NP-ZnO; B10, 10 mg/L bulk counterpart (B-ZnO); B100, 100 mg/L B-ZnO.
Fig. 3. Histochemical detection of H2O2 by amplex ultra-red reagent (A) and lipid peroxidation by bodipy reagent (B). CK, Control (water); NP10, 10 mg/L zinc oxide nanoparticle (NP-ZnO); NP100, 100 mg/L NP-ZnO; B10, 10 mg/L bulk counterpart (B-ZnO); B100, 100 mg/L B-ZnO.
Fig. 4. Indole-3-acetic acid (IAA) oxidation activity (A), guaiacol peroxidase (POX) activity (B) and native polyacrylamide gel electrophoresis of guaiacol peroxidase (C) from rice roots after 7 d of imbibition. In A and B, enzymatic activities are expressed as U/mg protein. CK, Control (water); NP10, 10 mg/L zinc oxide nanoparticle (NP-ZnO); NP100, 100 mg/L NP-ZnO; B10, 10 mg/L bulk counterpart (B-ZnO); B100, 100 mg/L B-ZnO. Data are Mean ± SE (n = 4). In C, B1, B2, B3 and B4 represent different bands of enzymatic activity.
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