Fig. 1. RNA-Seq analysis of Nipponbare and oserf922Cas9 plants before inoculation and at 24 h post-inoculation with Magnaporthe oryzae. A, Diagram of OsERF922 showing the position of the sgRNA target site. ATG and TGA represent start and stop codons, respectively. B, Sequence electropherograms of OsERF922 in wild type Nipponbare (NPB) and oserf922Cas9 mutants. The red rectangle highlights the mutations. C, Representative phenotypes of NPB and oserf922Cas9 mutants following spray inoculation with M. oryzae isolate RB22. D and E, Lesion number (D) and relative fungal biomass (E) of the inoculated leaves in C. F, Analysis of differentially expressed genes (DEGs) of leaves in NPB and oserf922Cas9 mutants before inoculation and at 24 h post-inoculation with RB22. G, Venn diagram showing the extent of overlapping DEGs in oserf922Cas9 mutants and NPB before inoculation and at 24 h post-inoculation with RB22. H, Heatmap representation of the expression levels of selected significantly up-regulated and down-regulated DEGs in oserf922Cas9 mutants and NPB-samples before inoculation and at 24 h post-inoculation with RB22. The genes are classified based on the function of their encoded proteins: E3 ligase, receptor kinase, cell wall-associated kinase, transcription factor, and others. The color scale indicates the expression level of genes from low (green) to high (red). I-L, Relative expression levels of DEGs from the RNA-Seq analysis encoding E3 ligase (I), receptor kinase (J) and transcription factors (K and L), as evaluated by qRT-PCR in leaves of NPB and oserf922Cas9 plants. The internal control gene was OsUbiquitin. Data in D, E and I-L are Mean ± SE (n = 3). Asterisks indicate statistically significant differences as determined by the Student’s t-test (*, P < 0.05; **, P < 0.01; ns, Not significant).