摘要: As the ‘Green Revolution’ gene, SD1 (encoding GA20ox2), has been widely applied to improve yield in rice breeding. However, research on its transcriptional regulation is limited. Here, we identified a transcription factor OsbZIP01 which can suppress the expression of SD1 and regulate gibberellin (GA) biosynthesis in rice. Knockout mutants of OsbZIP01 exhibited increased plant height, while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate. Furthermore, the semi-dwarf phenotype of OE-bZIP01 was caused by the reduced internode length, which was accompanied by a thin stem width. OsbZIP01 was mainly expressed in shoots, especially in leaves and sheaths. OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity. In addition, OsbZIP01 could directly bind to the promoter of the OsSD1 gene, and inhibit its transcription. The semi-dwarf phenotype of OsbZIP01 overexpression plants could be rescued by exogenous GA3. Meanwhile, the sd1 bzip01 double mutant showed a shorter shoot length compared with the wild-type, indicating that OsbZIP01 regulates plant growth mainly through the GA biosynthesis pathway. Collectively, OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription, thereby affecting plant growth and development