OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice

doi:10.1016/j.rsci.2023.11.007

摘要/Abstract

. [J]. Rice Science, 2024, 31(1): 77-86.

Fig. 1. Expression pattern of OsbZIP01 and its protein characterization. A, Gene expression atlas of OsbZIP01 in three representative indica varieties of China is from the CREP database (http://crep.ncpgr.cn/crep-cgi/home.pl). The expression value is log2-transformed. MH63, Minghui 63; SY63, Shanyou 63; ZS97, Zhenshan 97. B, Subcellular localization of OsbZIP01. DAPI, 4′,6-diamidino-2-phenylindole; GFP, Green fluorescent protein. Scale bars are 100 μm. C, Transactivation assay of OsbZIP01 in yeast cells. BK, pDEST-GBKT7; SD, Synthetic dropout medium; Trp, Tryptophan; Ade, Adenine; His, Histidine.

Fig. 2. Phenotypic characterization of Shennong 265 (SN265), cas-bzip01, and OE-bZIP01 transgenic plants. A, Schematic diagram of the sgRNA site of bZIP01 gene in SN265 and two mutants (cas-bzip01#1 and cas-bzip01#2) by CRISPR/Cas9 system. The red triangle indicates the sgRNA position. B, Phenotypes of SN265 and cas-bzip01 plants (cas-bzip01#1 and cas-bzip01#2) at the heading stage. Scale bar is 10 cm. C, Morphology of SN265 and overexpression lines (OE-bZIP01#6 and OE-bZIP01#9) at the maturity stage. Scale bar is 10 cm. D, Plant heights of SN265 and transgenic plants (cas-bzip01#1, cas-bzip01#2, OE-bZIP01#6, and OE-bZIP01#9) at the maturity stage. E, Seed germination phenotypes of SN265, cas-bzip01#1, and OE-bZIP01#6 plants. Sterile grains were sown on Murashige and Skoog plates, and photographs were taken at 48 h after germination at 30 ºC. Scale bar is 1 cm. F, Dynamic germination rates of SN265, cas-bzip01#1, and OE-bZIP01#6 after sown for 72 h. In D and F, Data are Mean ± SD of three independent experiments (n = 10 for each experiment). ** indicates the significant differences between transgenic plants and wild type (SN265) at the P < 0.01 by the Student’s t-test.

Fig. 3. Morphology comparison of cas-bzip01 and OE-bZIP01 transgenic plants. A and B, Comparison of internode lengths between wild type (Shennong 265, SN265), cas-bzip01#1, and OE-bZIP01#6 at the heading stage. Ⅰ, The first internode from the top; Ⅱ, The second internode from the top; Ⅲ, The third internode from the top; Ⅳ, The fourth internode from the top. Scale bar is 10 cm. C, Fast green-stained transverse sections of SN265, cas-bzip01#1, and OE-bZIP01#6 stems, showing differences in the diameter of the second internode from the top. Scale bars are 1 mm. D, Longitudinal sections of the first internode from the top of SN265, cas-bzip01#1, and OE-bZIP01#6. Scale bars are 120 μm. E, Comparison analysis of parenchyma cell lengths of the first internode from the top of SN265, cas-bzip01#1, and OE-bZIP01#6. In B and F, data are Mean ± SD of three independent experiments (n = 10 for each experiment). ** indicates the significant differences between transgenic plants and wild type at the P < 0.01 by the Student’s t-test.

Fig. 4. Phenotype response of wild type (Shennong 265, SN265), cas-bzip01, and OE-OsbZIP01 to exogenous GA. A, Seedling phenotype of SN265 and transgenic lines (cas-bzip01#1 and OE-bZIP01#6), which were treated with exogenous 10 μmol/L GA3 for 10 d. GA3(-) represents the absence of GA3, and GA3(+) represents the application of GA3. Scale bar is 5 cm. B, Shoot lengths of SN265 and transgenic lines (cas-bzip01#1 and OE-bZIP01#6). Data are Mean ± SD of three independent experiments (n = 10 for each experiment). Different lowercase letters indicate the significant differences between transgenic plants and wild type (P < 0.01, Student’s t-test).

Fig. 5. OsbZIP01 directly binds to promoter region of SD1. A and B, Relative expression levels of mRNA (A) and protein (B) of SD1 in the stems of wild type (Shennong 265, SN265), cas-bzip01#1, and OE-bZIP01#6 transgenic plants. C, OsbZIP01 binds in vitro to G-box in the SD1 promoter by electrophoretic mobility shift assay. For the assay, the radiolabeled probes were incubated with OsbZIP01 protein. Competitive (unlabeled) probe (50× and 100×), G-box probe were used as indicated. GST, Glutathione S-transferase. CDS, Coding sequence. D, Schematic diagrams of the effector and reporter constructs used the dual-luciferase reporter assays. E, Transactivity assay in rice proplasts. EIL1 protein positively regulates SD1 expression, which is used as the positive control. LUC, Luciferase. Data in A and E are Mean ± SD of three independent experiments (n = 10 for each experiment). ** indicates the significant differences between transgenic plants and wild type at the P < 0.01 by the Student’s t-test.

Fig. 6. Genetic relationship of OsbZIP01 and OsSD1. A and B, Shoot lengths of the single (sd1 and bzip01) and double (bzip01 sd1) mutant seedlings under Zhonghua 11 (ZH11) background with and without 10 μmol/L GA3 treatment [GA3(+) and GA3(-)] for 10 d. C and D, Phenotypic characterization (C) and shoot lengths (D) of the single (sd1 and bzip01) and double (bzip01 sd1) mutants at the maturity stage. In A and C, scale bars are 5 cm. In B and D, data are Mean ± SD of three independent experiments (n = 10 for each experiment), and different lowercase letters represent significant differences at the P < 0.05 by the Student’s t-test.

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