摘要： Rice often suffers from pests, diseases, and weeds throughout its life. Management of pests, diseases, and weeds with agrochemicals increases costs and results in pesticide residues. Developing new germplasm and varieties with multiple resistances is the most economical, sustainable, and efficient way to solve these problems. In this study, marker-assisted backcrossing was used to pyramid multiple resistance genes, and a penta-resistant restorer line, B2A1920, was developed and evaluated for agronomic traits and resistance to diseases, pests, and herbicides. It was confirmed that the developed restorer line B2A1920 contained the herbicide resistance gene Bar, lepidopteran pest resistance gene Cry2Aa, bacterial blight (BB) resistance gene Xa23, blast resistance gene Pi2, and brown planthopper (BPH) resistance genes Bph14 and Bph15, which conferred resistance to glufosinate, lepidopteran pests, BB, blast, and BPH, alongside possessing elite agronomic traits. Its hybrids, Gui A × B2A1920, Quan 9311A × B2A1920, Yexiang A × B2A1920, Xianglong A × B2A1920, Tiantai A × B2A1920, and Shen 9A × B2A1920, also exhibited penta-resistance and elite agronomic traits. These results indicated that a cytoplasmic male sterile restorer line with high combining ability and penta-resistance has been successfully developed.

关键词: hybrid rice, resistance, non-selective herbicide, rice blast, bacterial blight, lepidopteran pest, brown planthopper