Cloning of qHD8BAS, a Quantitative Trait Locus Regulating Heading Date in Rice

doi:10.1016/j.rsci.2024.12.009

摘要/Abstract

. [J]. Rice Science, 2025, 32(3): 283-286.

Fig. 1. qHD8 delays rice heading date under long-day (LD) conditions. A, Plant height, leaf length, leaf width, tiller number, panicle length, and yield per plant of NIL-qHD8HHZ and NIL-qHD8BAS under LD conditions. Data are Mean ± SE (n = 60). P values were calculated using the Student’s t-test. B, qHD8 locus was narrowed down to a 64.29-kb interval flanked by markers InDel-4 and InDel-6 on chromosome (chr.) 8, based on heading date, using 2 280 individuals from the BC7F2 population. LHD1 to LHD4 represent the recombinant lines, LHD5 represents NIL-qHD8BAS, and LHD6 represents NIL-qHD8HHZ. The aurantium and white rectangles represent homozygous chromosomal segments from Basmati Surkh 89-15 (BAS) and Huanghuazhan (HHZ) alleles, respectively. The column chart depicts the heading dates of LHD1 to LHD6 under LD conditions (sown on 26 May in Hangzhou, Zhejiang Province, China). ORF, Open reading frame. C, Sequencing results of qHD8 mutants in the NIL-qHD8BAS background, which were created using the CRISPR/Cas9 system. D, Phenotype of NIL-qHD8HHZ (with NIL-qHD8BAS treated as the wild type for comparison with its knockout lines) and its knockout lines (KO-qHD8-1 and KO-qHD8-2) under LD conditions (sown on 28 May in Hangzhou). Scale bar, 20 cm. E, Heading duration under LD conditions (sown on 28 May in Hangzhou). Data are Mean ± SE (n = 12). P values were calculated using the Student’s t-test.

参考文献 25

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