Effect of Hydroponically Supplied Selenium Forms on Cadmium and Nickel Accumulation in Rice

doi:10.1016/j.rsci.2025.03.005

摘要/Abstract

. [J]. Rice Science, 2025, 32(4): 561-574.

Fig. 1. Leaf (A) and root (B) dry weights of rice plants. Data were collected after 7 d of hydroponic cultivation with combinations of cadmium (Cd, 5 and 20 μmol/L, Cd5 and Cd20, respectively), nickel (Ni, 20 μmol/L), and selenium (Se, as selenate Se6+ or selenite Se4+, each at 5 μmol/L). CK, Control. Data are mean ± SE (n = 9). Different lowercase letters above bars identify significant differences among treatments after analysis of variance followed by Tukey’s HSD test (P < 0.05).

Fig. 2. Thiobarbituric acid reactive substances (TBARS) as an indictor of lipid peroxidation in hydroponic cultivation rice leaves. Data were collected after 7 d of hydroponic cultivation with combinations of cadmium (Cd, 5 and 20 μmol/L, Cd5 and Cd20, respectively), nickel (Ni, 20 μmol/L), and selenium (Se, as selenate Se6+ or selenite Se4+, each at 5 μmol/L). CK, Control. Data are mean ± SE (n = 3). Different lowercase letters above bars identify significant differences among treatments after analysis of variance followed by Tukey’s HSD test (P < 0.05).

Fig. 3. Cadmium (Cd), nickel (Ni), and selenium (Se) concentrations in leaves and roots of rice plants. A‒C, Cd (A), Ni (B), and Se (C) concentrations in leaves of rice plants. D‒F, Cd (D), Ni (E), and Se (F) concentrations in roots of rice plants. Data were collected after 7 d of hydroponic cultivation with combinations of cadmium (Cd, 5 and 20 μmol/L, Cd5 and Cd20, respectively), nickel (Ni, 20 μmol/L), and selenium (Se, as selenate Se6+ or selenite Se4+, each at 5 μmol/L). CK, Control.

Fig. 4. Translocation factors (TF) of cadmium (Cd, A), nickel (Ni, B), and selenium (Se, C) of rice plants. Data were collected after 7 d of hydroponic cultivation with combinations of cadmium (Cd, 5 and 20 μmol/L, Cd5 and Cd20, respectively), nickel (Ni, 20 μmol/L), and selenium (Se, as selenate Se6+ or selenite Se4+, each at 5 μmol/L). CK, Control.

Fig. 5. Distribution of selenium (Se), nickel (Ni), and iron (Fe) in roots of rice plants treated with Se and Ni. A‒C, Distribution of Se (A), Ni (B), and Fe (C) in roots of rice plants treated with 5 μmol/L Se6+ and 20 μmol/L Ni. D, Tricolor map showing simultaneous distribution of Se (red), Ni (green), and Fe (blue). Scale bars, 0.5 mm.

Fig. 6. Distribution of selenium (Se) and nickel (Ni) in leaves and roots of rice plants treated with 5 μmol/L Se6+ and different concentrations of cadmium (Cd) and Ni. A, C, E, G, I, K, M, and O, Distribution of Se in leaves and roots of rice plants treated with control (A), Se6+ (C), Se6+ and 5 μmol/L Cd (E), Se6+ and 20 μmol/L Cd (G), Ni (I), Se6+ and Ni (K), Se6+, 5 μmol/L Cd, and Ni (M), as well as Se6+, 20 μmol/L Cd, and Ni (O). B, D, F, H, J, L, N, and P, Distribution of Ni in leaves and roots of rice plants treated with control (B), Se6+ (D), Se6+ and 5 μmol/L Cd (F), Se6+ and 20 μmol/L Cd (H), Ni (J), Se6+ and Ni (L), Se6+, 5 μmol/L Cd, and Ni (N), as well as Se6+, 20 μmol/L Cd, and Ni (P). The concentrations used for Se6+ and Ni are 5 and 20 μmol/L, respectively. Scale bars, 5 mm.

Fig. 7. Distribution of selenium (Se) and nickel (Ni) in leaves and roots of rice plants treated with 5 μmol/L Se4+ and different concentrations of cadmium (Cd) and Ni. A, C, E, G, I, K, M, and O, Distribution of Se in leaves and roots of rice plants treated with control (A), Se4+ (C), Se4+ and 5 μmol/L Cd (E), Se4+ and 20 μmol/L Cd (G), Ni (I), Se4+ and Ni (K), Se4+, 5 μmol/L Cd, and Ni (M), as well as Se4+, 20 μmol/L Cd, and Ni (O). B, D, F, H, J, L, N, and P, Distribution of Ni in leaves and roots of rice plants treated with control (B), Se4+ (D), Se4+ and 5 μmol/L Cd (F), Se4+ and 20 μmol/L Cd (H), Ni (J), Se4+ and Ni (L), Se4+, 5 μmol/L Cd, and Ni (N), as well as Se4+, 20 μmol/L Cd, and Ni (P). The concentrations used for Se4+ and Ni are 5 and 20 μmol/L, respectively. Scale bars, 5 mm.

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