Rice Science

RICE SCIENCE ›› 2005, Vol. 12 ›› Issue (4): 233-237 .

• 研究报告 •    下一篇

Molecular Cloning and Characterization of Citrate Synthase Gene in Rice (Oryza sativa)

ZHANG Shan-shan1,2, MING Feng1,2, LU Qun3, GUO Bin1,2, SHEN Da-leng1,2   

  1. 1Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China; 2 Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Science, Fudan University, Shanghai 200433, China; 3 School of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China
  • 收稿日期:2005-09-09 出版日期:2005-12-28 发布日期:2005-12-28
  • 通讯作者: MING Feng
  • 基金资助:
    National Natural Science Foundation of China (No. 30300193).

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摘要： The full-length OsCS encoding citrate synthase was isolated from rice (Oryza sativa L. subsp. japonica). OsCS is 1477-bp long and encodes a 474 amino acid polypeptide. Its putative protein sequence is highly identical to Daucus carota, Nicotiana tabacum, Beta vulgaris subsp., Arabidopsis thaliana, and Citrus junos (＞70%). The deduced amino-terminal sequence of OsCS showes characteristics of mitochondrial targeting signal. Southern blot analysis using ORF of the OsCS as the probe indicated that this gene exists in multiple copies in rice genome. The band with predicated size of 82 kD was detected by Western blot after being induced by 0.4 mmol/L IPTG.  

关键词: citrate synthase, rice (Oryza sativa), gene, clone