Resistance to Bacterial Leaf Blight in a Somaclonal Rice Mutant HX-3 at Cellular Level

摘要/Abstract

摘要：

The interaction between rice host and its pathogen Xanthomonas oryzae pv. oryzae (Xoo) at cellular level was studied by using a resistant somaclonal mutant HX-3 and its susceptable donor Minghui 63. After inoculation with Xoo strain Zhe 173 (Chinese pathotype Ⅳ), the activity of superoxide dismutase (SOD) and peroxidase (POD) in the callus of Minghui 63 was increased dramatically, and the active oxygen(O2 ) was produced at a higher rate; Meanwhile, the callus grew slowly with the reduction of protein content. Compared to the activity of SOD and POD, the production rate of O2 and the fresh weight in HX-3 callus varied little after the inoculation. It could be proposed that there were great differences between the resistance of HX-3 and Mighui 63 at cellular level. There was no difference detected concerning resistance to bacterial leaf blight in HX-3 between the plant and the callus.

关键词: bacterial leaf blight, somaclonal mutant, active oxygen, resistance, cell level

GAO Dong-ying , ZHOU Yi-hong , HUANG Xue-qing , SUN Li-hua , LIU Ai-min . Resistance to Bacterial Leaf Blight in a Somaclonal Rice Mutant HX-3 at Cellular Level[J]. RICE SCIENCE.

参考文献

1 Lutts S, Bouharmont J, Kinet J M. Physiological chraracteris- ation of salt-resistant rice (Oryza sativa) somaclones. Austral J Bot, 1999, 47(6): 835–849.

2 Bertin P, Bouharmont J. Use of somaclonal variation and in vitro selection for chilling tolerance improvement in rice. Euphytica, 1997, 96(1): 135–142.

3 Ling D H, Vidhyaseharan P, Borrmeo E S, Zapata F J, Miao D H. In vitro screening of rice germplasm for resistance to brown spot disease using phytotoxin. Acta Genet Sin, 1986, 13(3): 194–200. (in Chinese with English abstract)

4 Sun L H, She J M, Lu X F. In vitro selection of Xanthomonas oryzae-resistant mutants in rice.Ⅰ. Induction of resistant callus and screening regenerated plants. Acta Genet Sin, 1986, 13(3): 188–193.

5 Mandal A B, Ansari M M, Sharma, T V R S, Bandyopadhyay A K. Somaclonal variation for disease resistance in indica rice. Rice Biotech Quarterly, 1995, 23: 8–9.

6 Yu H S, Wang J S, Fang Z D. Studies on interaction between rice varieties and strains of Xanthomonas oryzae pv. oryzae at cell level.Ⅱ. Interactions between rice callus and suspension cultures and races of Xanthomonas oryzae pv. oryzae. J Nanjing Agric Univ, 1994, 17(suppl): 7–12. (in Chinese with English abstract)

7 Gao D Y, Xu Z G, Chen Z Y, Sun L H, Sun Q M, Lu F, Hu B S, Liu Y F, Tang L H. Identification of a new gene for resistance to bacterial blight in a somaclonal mutant HX-3. Rice Genet Newsl, 2001, 18: 66–68.

8 Laval-Martin D L, Carre I A, Barbera S J, Edmunds J L N. Rhythmic changes in the activities of NAD kinase and NADP phosphatase in the achlorophyllous ZC mutants of Euglena gracilis Klebs (strain Z). Arch Biochem Biophys, 1990, 276(2): 433–441.

9 Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem, 1976, 72: 248–254.

10 Zhang Z L. The experimental Manual for Plant Physiology. Bejing: High Education Press, 1990. 154–155. (in Chinese)

11 Giannopolitis S, Ries S K. Superoxide dismutase: Occurrence

in higher plants. Plant Physiol, 1977, 59: 309–314.

12 Wang A G, Luo G H. Quantitative relation between the reaction of hydroxylamine and sproxid anion radicals in plants. Plant Physiol Comm, 1990, 29(6): 55–57. (in Chinese with English abstract)

13 Fang Z D. Methodology of Plant Pathology. Beijing: Agri- cultural Press, 1998. 382–387. (in Chinese)

14 Liu C Y, Li Z K, Quan M Q, Xie Y F, Zhang D P. A prelimi- nary screening of somaclonal variants of rice（Oryza sativa L.）resistant to Xathomonas oryzae Dowson. J Wuhan Bot Res, 1994, 12(1): 1–6. (in Chinese with English abstract)

15 Hemalatha R G, Jebaraj S, Raja J A J, Raguchander T, Ramanathan A, Samiyappan R, Balasubramanian P. Employ- ing a crude toxin preparation from Sarocladium oryzae as a molecular sieve to select sheath rot-resistant somaclones of rice. J Plant Biochem Biotech, 1999, 8(2): 75–80.

16 Wu Y X, Zeng F H, Wang R C. A preliminary study on the relationship between induced resistance to bacterial blight and deterense enzymes in hybrid rice seedling. Acta Phytopath Sin, 1996, 26(2): 127–131. (in Chinese with English abstract)

17 Kong F M, Xu Z G, Ma C H. Active oxygen reaction during early stage of interaction between Zhenshan 97 male sterile cytoplasm rice and Xanthomonas oryzae pv. oryzae. Chinese J Rice Sci, 1998, 12(3): 159–164. (in Chinese with English abstract)

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