Arbuscular Mycorrhizal Fungi Colonization in Upland Rice as Influenced by Agrochemical Application

doi:10.1016/S1672-6308(08)60095-5

摘要/Abstract

摘要： Mycorrhizal status of rice under upland conditions was studied using potted seedlings. Percentage of arbuscular mycorrhizal fungi (AMF) root colonization varied between 17.35% and 37.18% over an age series of 7 to 70 days old rice plants. AMF root colonization was increased up to 35–42 days, beyond which the root colonization steadily declined. The vesicles appeared after two weeks and reached their maximum intensity on the 35th day. The arbuscules were formed late on the 42nd day (2.93%) and slightly varied up to the 70th day (3.03%). Higher dosage of urea application suppressed plant growth whereas the superphosphate treatment had no marked impact on plant growth. Generally, application of these agrochemicals registered less influence on the hyphal colonization of AMF in rice plants, whereas arbuscular colonization was adversely affected by higher dosages of fertilizers. There were pronounced decreases in both the plant growth and their AMF colonization due to the application of systemic fungicides, carbendazim and thiophanate methyl. The application of single sprays of fungicides was less deleterious over multiple sprays.

关键词: Oryza sativa, arbuscular mycorrhizal fungi, agrochemical, upland rice

Velu RAJESHKANNAN, Chettipalayam Samiappan SUMATHI, Sellamuthu MANIAN. Arbuscular Mycorrhizal Fungi Colonization in Upland Rice as Influenced by Agrochemical Application[J]. RICE SCIENCE, 2009, 16(4): 307-313 .

参考文献

1 Ammani K, Venkateswarlu K, Rao A S. Development of vesicular arbuscular mycorrhizal fungi on upland rice variety. Curr Sci, 1985, 59: 1120–1122.
2 Sivaprasad P, Sulochana K K, Salem M A. Vesicular- arbuscular mycorrhizae (VAM) colonization in lowland rice roots and its effect on growth and yield. Intl Rice Res Newsl, 1990, 15: 14–15.
3 Furlan V, Bernier-Cardouu M. Effects of N, P and K on formation of vesicular-arbuscular mycorrhizae, growth and mineral content of onion. Plant & Soil, 1989, 113: 167–174.
4 Jasper D A, Robson A D, Abbott L K. Phosphorus and the formation of vesicular-arbuscular mycorrhizas. Soil Biol Biochem, 1979, 1: 501–505.
5 Abbott L K, Robson A D, de Boer G. The effect of phosphorus on the formation of hyphae in the soil by the vesicular- arbuscular mycorrhizal fungus Glomus fasciculatus. New Phytol, 1984, 97: 191–196.
6 Dhillion S S, Amornpan L. The influence of inorganic nutrients fertilization on the growth nutrient composition and vesicular-arbuscular mycorrhizal colonization of pre-transplant stage rice (Oryza sativa L.) plants. Biol Fert Soils, 1992, 13: 85–91.
7 Dhillion S S, Ampornpan L. Influence of mycorrhizal association and inorganic nutrients on early growth of rice. Intl Rice Res Newsl, 1990, 15: 16–17.
8 McGonigle T P, Miller M H, Evans D G, Fairchild G L, Swan J A. A new method which gives an objective measure of colonisation by vesicular arbuscular mycorrhizal fungi. New Phytol, 1990, 115: 495–501.
9 Jackson M L. Soil Chemical Analysis. New Delhi: Prentice Hall of India Pvt Ltd, 1973.
10 Maclean J L, Dawe D C, Hardy B, Hettel G P. Rice Almanac. Los Banos, Philippines: IRRI, WARDA, CIAT and FAO, 2002: 1–10.
11 Bouman B A M, Humphreys E, Tuong T P, Baker R B. Rice and water. Adv Agron, 2007, 92: 187–237.
12 Manjunath A, Mohan R, Raj J, Bagyaraj D J. Vesicular arbuscular mycorrhizas in cultivars of rice. J Soil Biol Ecol, 1981, 1: 1–4.
13 Brown M B, Quimio T H, Decartro A M. Vesicular-arbuscular mycorrhizas associated with upland rice (Oryza sativa L.). Agriculturist, 1988, 71: 317–332.
14 Baby U I, Manibhushanrao K. Vesicular-arbuscular mycorrhizal fungi of rice field soils. Acta Bot Ind, 1992, 20: 10–15.
15 de Carvalho-Niebel F, Timmers A C J, Chabaud M, Defaux- Petras A, Barker D G. The Nod factor-elicited annexin MtAnn1 is preferentially localized at the nuclear periphery in symbiotically activated root tissues of Medicago truncatula. Plant J, 2002, 32: 343–352.
16 Gianinazzi-Pearson V. Plant cell responses to arbuscular mycorrhizal fungi: Getting to the roots of the symbiosis. Plant Cell, 1996, 8: 1871–1883.
17 Jalaluddin M, Anwar Q M K. VAM fungi in wheat and rice fields. Pak J Bot, 1991, 23: 115–122.
18 Mosse B, Bowen G D. A key to the recognition of some Endogone spore types. Trans Brit Mycol Soc, 1968, 52: 469– 483.
19 Kuckelmann H W. Effects of fertilizers, soils, soil tillage and plant species on the frequency of Endogone chlamydospores and mycorrhizal infection in arable soils. In: Sanders F E, Mosse B, Tinker P B. Endomycorrhizas. London, New York: Academic Press, 1975: 511–525.
20 Baltruschat H, Deane H W Y. The occurrence of vesicular arbuscular mycorrhizal in agro-ecosystems: I. Influence of nitrogen fertilization and green manure in continuous monoculture and in crop rotation on the inoculum potential of winter wheat. Plant & Soil, 1988, 107: 279–284.
21 An Z Q, Hendrix J W, Hershman D E, Ferriss R S, Henson G T. The influence of crop rotation and soil fumigation on a mycorrhizal fungal community associated with soybean. Mycorrhiza, 1993, 3: 171–182.
22 Purakayastha T J, Chhonkar P K. Influence of vesicular- arbuscular mycorrhizal fungi (Glomus etunicatum L.) on mobilization of zinc in wetland rice (Oryza sativa L.). Biol Fert Soils, 2001, 33: 323–327.
23 Zhang X H, Zhu Y G, Chen B D, Lin A J, Smith S E, Smith F A. Arbuscular mycorrhizal fungi contribute to resistance of upland rice to combined metal contamination of soil. J Plant Nutr, 2005, 28: 2065–2077.
24 Gao X, Kuyper T W, Zou C, Zhang F S, Hoffland E. Mycorrhizal responsiveness of aerobic rice genotypes is negatively correlated with their zinc uptake when nonmycorrhizal. Plant & Soil, 2007, 290: 283–291.
25 Herdler S, Kreuzer K, Scheu S, Bonkowski M. Interactions between arbuscular mycorrhizal fungi (Glomus intraradices, Glomeromycota) and amoebae (Acanthamoeba castellanii, Protozoa) in the rhizosphere of rice (Oryza sativa). Soil Biol Biochem, 2008, 40: 660–668.
26 Phongpan S, Mosier A R. Effect of crop residue management on nitrogen dynamics and balance in a lowland rice cropping system. Nut Cycl Agroecosyst, 2003, 66: 133–142.
27 Thomson B D, Robson A D, Abbott L K. Effects of phosphorus on the formation of mycorrhizas by Gigaspora calospora and Glomus fasciculatum in relation to root carbohydrates. New Phytol, 1985, 103: 751–765.
28 Li Y, Ran W, Zhang R, Sun S, Xu G. Facilitated legume nodulation, phosphate uptake and nitrogen transfer by arbuscular inoculation in an upland rice and mung bean intercropping system. Plant & Soil, 2009, 315: 285–296.
29 Dhillon S S. Host endophyte specificity of vesicular arbuscular mycorrhizal colonization of Oryza sativa L. at the pre- transplant stage in low or high phosphorus soil. Soil Biol Biochem, 1992, 24: 405–411.
30 Kling M, Jakobsen I. Direct application of carbendazim and propiconazole at field rates to the external mycelium of three arbuscular mycorrhizal fungi species: Effect on 32P transport and succinate dehydrogenase activity. Mycorrhiza, 1997, 7: 33–37.
31 Burke D J, Hamerlynck E P, Hahn D. Effect of arbuscular mycorrhizae on soil microbial populations and associated plant performance of the salt marsh grass Spartina patens. Plant & Soil, 2002, 239: 141–154.

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