Rice Science ›› 2016, Vol. 23 ›› Issue (2): 69-77.DOI: 10.1016/j.rsci.2016.02.002
收稿日期:
2015-06-03
接受日期:
2015-10-08
出版日期:
2016-04-10
发布日期:
2015-12-31
. [J]. Rice Science, 2016, 23(2): 69-77.
Fig. 1. Molecular detection of Stv-bi and Wx-mq genes for parts of plants in BC3F1 generation.M, 2 kb DNA marker; Lane 1, Wuyujing 3; Lane 2, Kanto 194; Lane 3, F1 (Wuyujing 3/Kanto 194); Lanes 4 to 24, Parts of plants in BC3F1 generation.
Line/Variety | Incident rate of rice stripe disease (%) | Resistance level | |
---|---|---|---|
Nanjing, Jiangsu Province | Yandu, Jiangsu Province | ||
K01 | 0.00 ± 0.00 | 0.20 ± 0.20 | Highly resistant |
K02 | 1.41 ± 0.21 | 1.55 ± 0.30 | Highly resistant |
K03 | 1.15 ± 0.31 | 1.48 ± 0.32 | Highly resistant |
K04 | 0.20 ± 0.00 | 0.41 ± 0.20 | Highly resistant |
K05 | 0.26 ± 0.23 | 0.94 ± 0.23 | Highly resistant |
K06 | 0.26 ± 0.11 | 0.87 ± 0.23 | Highly resistant |
K07 | 2.73 ± 0.50 | 3.74 ± 0.51 | Highly resistant |
K08 | 0.07 ± 0.12 | 0.40 ± 0.20 | Highly resistant |
K09 | 0.88 ± 0.31 | 1.48 ± 0.31 | Highly resistant |
K10 | 0.79 ± 0.39 | 1.28 ± 0.12 | Highly resistant |
Kanto 194 | 0.28 ± 0.11 | 0.66 ± 0.18 | Highly resistant |
Wuyujing 3 | 64.82 ± 0.80 | 68.97 ± 0.41 | Highly susceptible |
Table 1 Comparison of resistance of rice stripe disease for improved lines and Wuyujing 3 (Mean ± SD).
Line/Variety | Incident rate of rice stripe disease (%) | Resistance level | |
---|---|---|---|
Nanjing, Jiangsu Province | Yandu, Jiangsu Province | ||
K01 | 0.00 ± 0.00 | 0.20 ± 0.20 | Highly resistant |
K02 | 1.41 ± 0.21 | 1.55 ± 0.30 | Highly resistant |
K03 | 1.15 ± 0.31 | 1.48 ± 0.32 | Highly resistant |
K04 | 0.20 ± 0.00 | 0.41 ± 0.20 | Highly resistant |
K05 | 0.26 ± 0.23 | 0.94 ± 0.23 | Highly resistant |
K06 | 0.26 ± 0.11 | 0.87 ± 0.23 | Highly resistant |
K07 | 2.73 ± 0.50 | 3.74 ± 0.51 | Highly resistant |
K08 | 0.07 ± 0.12 | 0.40 ± 0.20 | Highly resistant |
K09 | 0.88 ± 0.31 | 1.48 ± 0.31 | Highly resistant |
K10 | 0.79 ± 0.39 | 1.28 ± 0.12 | Highly resistant |
Kanto 194 | 0.28 ± 0.11 | 0.66 ± 0.18 | Highly resistant |
Wuyujing 3 | 64.82 ± 0.80 | 68.97 ± 0.41 | Highly susceptible |
Line/Variety | PH (cm) | PL (cm) | PP | FGPL | SPL | FGPA | SPA | SSR (%) | TGW (g) | GWP (g) |
---|---|---|---|---|---|---|---|---|---|---|
K01 | 97.9 | 15.7 | 14.3 | 1 397.9 | 1 452.2 | 98.6 | 102.6 | 96.3 | 25 | 33.6 |
K02 | 99.4 | 15 | 14.6 | 1 421.1 | 1 475.3 | 97.2 | 101 | 96.2 | 24.7 | 32.7 |
K03 | 99.1 | 15.4 | 14.3 | 1 289.6 | 1 354.7 | 90.8 | 95.5 | 95.2 | 24.7 | 31.2 |
K04 | 99.8 | 15.9 | 14.9 | 1 336.7 | 1 383.6 | 89.3 | 92.6 | 96.5 | 24.9 | 32.3 |
K05 | 99.7 | 15.5 | 13.4 | 1 249.0 | 1 291.6 | 93.6 | 96.8 | 96.7 | 25.9 | 32.1 |
K06 | 101.5 | 15.3 | 13.9 | 1 324.7 | 1 372.3 | 95.3 | 98.8 | 96.4 | 25.5 | 33.7 |
K07 | 100.2 | 15.9 | 13.4 | 1 288.7 | 1 329.5 | 96.3 | 99.4 | 96.9 | 25.1 | 32.2 |
K08 | 100.2 | 15.1 | 14 | 1 322.9 | 1 390.8 | 94.7 | 99.6 | 95.1 | 25.5 | 32.8 |
K09 | 100 | 15.6 | 13.7 | 1 292.0 | 1 347.2 | 94.3 | 98.4 | 95.8 | 25.6 | 33 |
K10 | 99.5 | 15.8 | 14.7 | 1 348.4 | 1 391.1 | 92 | 95 | 96.7 | 24.7 | 33.2 |
Wuyujing 3 | 98.6 | 15.6 | 13.6 | 1 258.5 | 1 325.4 | 93.6 | 98.6 | 95 | 25.9 | 32.8 |
Table 2 Comparison of main agronomic and yield traits for improved lines and Wuyujing 3.
Line/Variety | PH (cm) | PL (cm) | PP | FGPL | SPL | FGPA | SPA | SSR (%) | TGW (g) | GWP (g) |
---|---|---|---|---|---|---|---|---|---|---|
K01 | 97.9 | 15.7 | 14.3 | 1 397.9 | 1 452.2 | 98.6 | 102.6 | 96.3 | 25 | 33.6 |
K02 | 99.4 | 15 | 14.6 | 1 421.1 | 1 475.3 | 97.2 | 101 | 96.2 | 24.7 | 32.7 |
K03 | 99.1 | 15.4 | 14.3 | 1 289.6 | 1 354.7 | 90.8 | 95.5 | 95.2 | 24.7 | 31.2 |
K04 | 99.8 | 15.9 | 14.9 | 1 336.7 | 1 383.6 | 89.3 | 92.6 | 96.5 | 24.9 | 32.3 |
K05 | 99.7 | 15.5 | 13.4 | 1 249.0 | 1 291.6 | 93.6 | 96.8 | 96.7 | 25.9 | 32.1 |
K06 | 101.5 | 15.3 | 13.9 | 1 324.7 | 1 372.3 | 95.3 | 98.8 | 96.4 | 25.5 | 33.7 |
K07 | 100.2 | 15.9 | 13.4 | 1 288.7 | 1 329.5 | 96.3 | 99.4 | 96.9 | 25.1 | 32.2 |
K08 | 100.2 | 15.1 | 14 | 1 322.9 | 1 390.8 | 94.7 | 99.6 | 95.1 | 25.5 | 32.8 |
K09 | 100 | 15.6 | 13.7 | 1 292.0 | 1 347.2 | 94.3 | 98.4 | 95.8 | 25.6 | 33 |
K10 | 99.5 | 15.8 | 14.7 | 1 348.4 | 1 391.1 | 92 | 95 | 96.7 | 24.7 | 33.2 |
Wuyujing 3 | 98.6 | 15.6 | 13.6 | 1 258.5 | 1 325.4 | 93.6 | 98.6 | 95 | 25.9 | 32.8 |
Line/Variety | Lishui, Jiangsu Province | Taizhou, Jiangsu Province | Average | |||
---|---|---|---|---|---|---|
Yield (kg/hm2) | Order | Yield (kg/hm2) | Order | Yield (kg/hm2) | Order | |
K01 | 7 543.5 | 5 | 10 680.0 | 4 | 9 111.8 | 3 |
K02 | 7 362.0 | 10 | 10 740.0 | 3 | 9 051.0 | 5 |
K03 | 7 335.0 | 11 | 10 132.5 | 10 | 8 733.8 | 11 |
K04 | 7 422.0 | 9 | 10 822.5 | 2 | 9 122.3 | 2 |
K05 | 7 485.0 | 6 | 10 035.0 | 11 | 8 760.0 | 10 |
K06 | 7 755.0 | 2 | 10 732.5 | 1 | 9 243.8 | 1 |
K07 | 7 887.0 | 1 | 10 155.0 | 9 | 9 021.0 | 6 |
K08 | 7 618.5 | 4 | 10 305.0 | 7 | 8 961.8 | 8 |
K09 | 7 740.0 | 3 | 10 432.5 | 6 | 9 086.3 | 4 |
K10 | 7 437.0 | 7 | 10 545.0 | 5 | 8 991.0 | 7 |
Wuyujing 3 | 7 429.5 | 8 | 10 257.0 | 8 | 8 843.3 | 9 |
Table 3 Comparison of actual yields for improved lines and Wuyujing 3.
Line/Variety | Lishui, Jiangsu Province | Taizhou, Jiangsu Province | Average | |||
---|---|---|---|---|---|---|
Yield (kg/hm2) | Order | Yield (kg/hm2) | Order | Yield (kg/hm2) | Order | |
K01 | 7 543.5 | 5 | 10 680.0 | 4 | 9 111.8 | 3 |
K02 | 7 362.0 | 10 | 10 740.0 | 3 | 9 051.0 | 5 |
K03 | 7 335.0 | 11 | 10 132.5 | 10 | 8 733.8 | 11 |
K04 | 7 422.0 | 9 | 10 822.5 | 2 | 9 122.3 | 2 |
K05 | 7 485.0 | 6 | 10 035.0 | 11 | 8 760.0 | 10 |
K06 | 7 755.0 | 2 | 10 732.5 | 1 | 9 243.8 | 1 |
K07 | 7 887.0 | 1 | 10 155.0 | 9 | 9 021.0 | 6 |
K08 | 7 618.5 | 4 | 10 305.0 | 7 | 8 961.8 | 8 |
K09 | 7 740.0 | 3 | 10 432.5 | 6 | 9 086.3 | 4 |
K10 | 7 437.0 | 7 | 10 545.0 | 5 | 8 991.0 | 7 |
Wuyujing 3 | 7 429.5 | 8 | 10 257.0 | 8 | 8 843.3 | 9 |
Fig. 3. Field performance and appearance quality for two improved lines and Wuyujing 3.A and D, Improved line K01; B and E, Improved line K04; C and F, Wuyujing 3.
Line/Variety | Milling quality | Appearance quality | Eating quality | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Brown rice rate (%) | Milled rice rate (%) | Head milled rice rate (%) | Grain length | Length/width ratio | Chalky grain rate (%) | Degree of chalkiness (%) | Gel consistency | Alkali spreading value | Amylose content (%) | |
K01 | 79.5 | 71.8 | 68.5 | 4.72 | 1.65 | 8.5** | 1.2** | 80.8** | 5.8 | 8.2** |
K02 | 78.7 | 70.2 | 65.8 | 4.46 | 1.65 | 24.2** | 4.2** | 81.2** | 5.8 | 8.2** |
K03 | 78.7 | 70.9 | 68 | 4.53 | 1.68 | 13.7** | 2.9** | 79.7** | 5.8 | 7.5** |
K04 | 79.8 | 71.9 | 68.1 | 4.53 | 1.66 | 14.7** | 2.9** | 80.1** | 5.7 | 7.5** |
K05 | 80.3 | 72.5 | 68.6 | 4.98 | 1.63 | 23.9** | 4.0** | 81.1** | 5.8 | 7.6** |
K06 | 80.3 | 71.8 | 68.7 | 4.95 | 1.63 | 14.1** | 2.1** | 81.2** | 5.8 | 7.6** |
K07 | 79.2 | 71.2 | 68.5 | 4.74 | 1.69 | 13.7** | 2.9** | 81.5** | 5.8 | 8.1** |
K08 | 79.5 | 71.2 | 68.2 | 4.93 | 1.66 | 12.2** | 2.2** | 81.7** | 5.7 | 8.7** |
K09 | 79.7 | 72.4 | 69.2 | 4.93 | 1.67 | 18.0** | 4.0** | 81.7** | 5.7 | 8.9** |
K10 | 78.7 | 71.6 | 69.1 | 4.7 | 1.66 | 21.8** | 4.5** | 82.2** | 5.8 | 8.9** |
Wuyujing 3 | 78.8 | 70.1 | 67.1 | 4.99 | 1.61 | 45.2 | 9.2 | 75.8 | 5.8 | 14.2 |
Table 4 Comparison of main quality traits for improved lines and Wuyujing 3.
Line/Variety | Milling quality | Appearance quality | Eating quality | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Brown rice rate (%) | Milled rice rate (%) | Head milled rice rate (%) | Grain length | Length/width ratio | Chalky grain rate (%) | Degree of chalkiness (%) | Gel consistency | Alkali spreading value | Amylose content (%) | |
K01 | 79.5 | 71.8 | 68.5 | 4.72 | 1.65 | 8.5** | 1.2** | 80.8** | 5.8 | 8.2** |
K02 | 78.7 | 70.2 | 65.8 | 4.46 | 1.65 | 24.2** | 4.2** | 81.2** | 5.8 | 8.2** |
K03 | 78.7 | 70.9 | 68 | 4.53 | 1.68 | 13.7** | 2.9** | 79.7** | 5.8 | 7.5** |
K04 | 79.8 | 71.9 | 68.1 | 4.53 | 1.66 | 14.7** | 2.9** | 80.1** | 5.7 | 7.5** |
K05 | 80.3 | 72.5 | 68.6 | 4.98 | 1.63 | 23.9** | 4.0** | 81.1** | 5.8 | 7.6** |
K06 | 80.3 | 71.8 | 68.7 | 4.95 | 1.63 | 14.1** | 2.1** | 81.2** | 5.8 | 7.6** |
K07 | 79.2 | 71.2 | 68.5 | 4.74 | 1.69 | 13.7** | 2.9** | 81.5** | 5.8 | 8.1** |
K08 | 79.5 | 71.2 | 68.2 | 4.93 | 1.66 | 12.2** | 2.2** | 81.7** | 5.7 | 8.7** |
K09 | 79.7 | 72.4 | 69.2 | 4.93 | 1.67 | 18.0** | 4.0** | 81.7** | 5.7 | 8.9** |
K10 | 78.7 | 71.6 | 69.1 | 4.7 | 1.66 | 21.8** | 4.5** | 82.2** | 5.8 | 8.9** |
Wuyujing 3 | 78.8 | 70.1 | 67.1 | 4.99 | 1.61 | 45.2 | 9.2 | 75.8 | 5.8 | 14.2 |
Line/Variety | Odor | Appearance | Palatability | Fragrance | Comprehensive score | Taste value |
---|---|---|---|---|---|---|
K01 | 0.1 | 0.8 | 1.40** | 0.90* | 1.50** | 0.96** |
K02 | 0.2 | 1.10* | 1.30* | 1.20** | 1.50** | 1.13** |
K03 | 0.1 | 0.4 | 0.7 | 0.5 | 0.8 | 0.51 |
K04 | 0.1 | 0.90* | 0.9 | 0.7 | 0.9 | 0.74 |
K05 | 0.1 | 0.7 | 0.6 | 0.6 | 0.7 | 0.58 |
K06 | 0 | 0.5 | 1 | 1.00* | 1.38* | 0.84* |
K07 | 0.1 | 1.20** | 1.70** | 1.60** | 1.90** | 1.42** |
K08 | 0.1 | 1.10* | 1.40** | 0.90* | 1.50** | 1.02** |
K09 | 0 | 0.90* | 1.30* | 1.30** | 1.40* | 1.10** |
K10 | 0.2 | 0.90* | 1.30* | 1.20** | 1.20* | 1.16** |
Wuyujing 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Table 5 Score of taste indices for improved lines and Wuyujing 3.
Line/Variety | Odor | Appearance | Palatability | Fragrance | Comprehensive score | Taste value |
---|---|---|---|---|---|---|
K01 | 0.1 | 0.8 | 1.40** | 0.90* | 1.50** | 0.96** |
K02 | 0.2 | 1.10* | 1.30* | 1.20** | 1.50** | 1.13** |
K03 | 0.1 | 0.4 | 0.7 | 0.5 | 0.8 | 0.51 |
K04 | 0.1 | 0.90* | 0.9 | 0.7 | 0.9 | 0.74 |
K05 | 0.1 | 0.7 | 0.6 | 0.6 | 0.7 | 0.58 |
K06 | 0 | 0.5 | 1 | 1.00* | 1.38* | 0.84* |
K07 | 0.1 | 1.20** | 1.70** | 1.60** | 1.90** | 1.42** |
K08 | 0.1 | 1.10* | 1.40** | 0.90* | 1.50** | 1.02** |
K09 | 0 | 0.90* | 1.30* | 1.30** | 1.40* | 1.10** |
K10 | 0.2 | 0.90* | 1.30* | 1.20** | 1.20* | 1.16** |
Wuyujing 3 | 0 | 0 | 0 | 0 | 0 | 0 |
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