Fine-Mapping of qTGW1.2a, a Quantitative Trait Locus for 1000-Grain Weight in Rice

doi:10.1016/j.rsci.2019.04.004

摘要/Abstract

. [J]. Rice Science, 2019, 26(4): 220-228.

图/表 8

参考文献 42

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Year	Population		Segregating region		Number of lines
Year	Generation	Name	Marker	Physical position (bp)	NIL^ZS97	NIL^MY46
2014	BC₂F₁₂	W14-1	RM212-Wn33252	33 053 493-33 252 459	20	20
		W14-2	RM212-Wn33252	33 053 493-33 252 459	20	19
		W14-3	Wn33252-Wn33304	33 252 459-33 304 754	40	40
2016	BC₂F₁₄	W16-1	Wn33011-Wn33164	33 011 637-33 164 422	19	14
		W16-2	Wn33186-Wn33252	33 186 760-33 252 459	33	33
2017	BC₂F₁₆	W17-1	Wn33011-Wn33186	33 011 637-33 186 760	33	34
		W17-2	RM212-Wn33252	33 053 493-33 252 459	33	34
		W17-3	Wn33186-Wn33252	33 186 760-33 252 459	34	35
2018	BC₂F₁₇	W18-1	Wn32997-Wn33011	32 996 766-33 011 637	30	30
		W18-2	RM212-Wn33186	33 053 493-33 186 760	28	28
		W18-3	Wn33089-Wn33252	33 089 090-33 252 459	29	30
NIL^ZS97 and NIL^MY46 are near isogenic lines (NILs) having Zhenshan 97 and Milyang 46 homozygous genotypes in the segregating regions, respectively.

Year	Population		Segregating region		Number of lines
Year	Generation	Name	Marker	Physical position (bp)	NIL^ZS97	NIL^MY46
2014	BC₂F₁₂	W14-1	RM212-Wn33252	33 053 493-33 252 459	20	20
		W14-2	RM212-Wn33252	33 053 493-33 252 459	20	19
		W14-3	Wn33252-Wn33304	33 252 459-33 304 754	40	40
2016	BC₂F₁₄	W16-1	Wn33011-Wn33164	33 011 637-33 164 422	19	14
		W16-2	Wn33186-Wn33252	33 186 760-33 252 459	33	33
2017	BC₂F₁₆	W17-1	Wn33011-Wn33186	33 011 637-33 186 760	33	34
		W17-2	RM212-Wn33252	33 053 493-33 252 459	33	34
		W17-3	Wn33186-Wn33252	33 186 760-33 252 459	34	35
2018	BC₂F₁₇	W18-1	Wn32997-Wn33011	32 996 766-33 011 637	30	30
		W18-2	RM212-Wn33186	33 053 493-33 186 760	28	28
		W18-3	Wn33089-Wn33252	33 089 090-33 252 459	29	30
NIL^ZS97 and NIL^MY46 are near isogenic lines (NILs) having Zhenshan 97 and Milyang 46 homozygous genotypes in the segregating regions, respectively.

Marker	Forward primer (5'-3')	Reverse primer (5'-3')
Wn32886	CGTGTTACTAACCCAGTCAACTCAGG	CAATAGTAGCAGCTAAATTGGCGTTC
Wn32922	AAGAAGAACATTTGCTCACAG	TGCAGTCTATTGTTGACCA
Wn32997	GTCAACCCAAATGTAGTAAGC	CCGCCGAGGTGAAGTTGTAG
Wn33011	TACAATCTACTATCCCGGACT	CTCGCAATTTACATGCAAACT
Wn33072	CTGGACAACATCCCCATGCT	GACCGGAAACCAAATACACCC
Wn33084	TTGTGACCGCATTAATAAGCC	TAATATTTTATGCGTAACTTG
Wn33089	GCTGCCGTGCAATTCGTAGTT	GAGGGATCGCGATGGGGGAGT
Wn33164	TACGCACTTGCCCTTAGATGTC	TTTCCTATCTATTTATTGCCCTA
Wn33186	AGTTAGTGCAGCAATCTACGTCCT	AGCTCCTCCAGTGACGATACGG
Wn33252	GCATGTATCAAAGATTCGATGAGA	TGAAAACTCATGGCTACGCT
Wn33268	CGTATATGCTCGTGACTCTG	ATCAGCGAGACAACGACTTCC
Wn33304	ATCCAAAATAGTTGAGGGCAT	TTATTTAGCTAGATTATTATAGGCTGG

Marker	Forward primer (5'-3')	Reverse primer (5'-3')
Wn32886	CGTGTTACTAACCCAGTCAACTCAGG	CAATAGTAGCAGCTAAATTGGCGTTC
Wn32922	AAGAAGAACATTTGCTCACAG	TGCAGTCTATTGTTGACCA
Wn32997	GTCAACCCAAATGTAGTAAGC	CCGCCGAGGTGAAGTTGTAG
Wn33011	TACAATCTACTATCCCGGACT	CTCGCAATTTACATGCAAACT
Wn33072	CTGGACAACATCCCCATGCT	GACCGGAAACCAAATACACCC
Wn33084	TTGTGACCGCATTAATAAGCC	TAATATTTTATGCGTAACTTG
Wn33089	GCTGCCGTGCAATTCGTAGTT	GAGGGATCGCGATGGGGGAGT
Wn33164	TACGCACTTGCCCTTAGATGTC	TTTCCTATCTATTTATTGCCCTA
Wn33186	AGTTAGTGCAGCAATCTACGTCCT	AGCTCCTCCAGTGACGATACGG
Wn33252	GCATGTATCAAAGATTCGATGAGA	TGAAAACTCATGGCTACGCT
Wn33268	CGTATATGCTCGTGACTCTG	ATCAGCGAGACAACGACTTCC
Wn33304	ATCCAAAATAGTTGAGGGCAT	TTATTTAGCTAGATTATTATAGGCTGG

Generation	Name	Trait	Phenotype (Mean ± SD)		P value	A	R² (%)
Generation	Name	Trait	NIL^ZS97	NIL^MY46	P value	A	R² (%)
BC₂F₁₂	W14-1	TGW (g)	29.30 ± 0.27	29.77 ± 0.30	<0.0001	0.23	26.15
		GL (mm)	8.515 ± 0.040	8.519 ± 0.041	0.7831
		GW (mm)	3.278 ± 0.016	3.304 ± 0.020	<0.0001	0.013	21.60
	W14-2	TGW (g)	29.27 ± 0.27	29.99 ± 0.27	<0.0001	0.36	41.65
		GL (mm)	8.561 ± 0.042	8.576 ± 0.034	0.2397
		GW (mm)	3.303 ± 0.019	3.355 ± 0.019	<0.0001	0.026	42.38
	W14-3	TGW (g)	29.44 ± 0.28	29.33 ± 0.34	0.1240
		GL (mm)	8.545 ± 0.051	8.557 ± 0.049	0.3004
		GW (mm)	3.242 ± 0.021	3.226 ± 0.026	0.0038	-0.008	6.00
BC₂F₁₄	W16-1	TGW (g)	27.51 ± 0.43	28.28 ± 0.37	<0.0001	0.38	31.23
		GL (mm)	8.261 ± 0.048	8.319 ± 0.041	0.0013	0.029	20.34
		GW (mm)	3.079 ± 0.017	3.111 ± 0.016	<0.0001	0.016	32.37
	W16-2	TGW (g)	27.30 ± 0.46	27.32 ± 0.39	0.8195
		GL (mm)	8.302 ± 0.043	8.291 ± 0.045	0.3423
		GW (mm)	3.064 ± 0.021	3.065 ± 0.024	0.8812
BC₂F₁₆	W17-1	TGW (g)	26.40 ± 0.32	26.99 ± 0.31	<0.0001	0.29	35.39
		GL (mm)	8.207 ± 0.042	8.246 ± 0.038	0.0002	0.019	11.55
		GW (mm)	3.028 ± 0.019	3.070 ± 0.025	<0.0001	0.021	35.80
	W17-2	TGW (g)	26.38 ± 0.26	26.94 ± 0.29	<0.0001	0.28	33.59
		GL (mm)	8.195 ± 0.037	8.263 ± 0.036	<0.0001	0.034	28.15
		GW (mm)	3.042 ± 0.019	3.064 ± 0.021	<0.0001	0.011	16.21
	W17-3	TGW (g)	26.77 ± 0.23	26.78 ± 0.23	0.8769
		GL (mm)	8.306 ± 0.027	8.302 ± 0.031	0.5432
		GW (mm)	3.031 ± 0.016	3.031 ± 0.014	0.9496
BC₂F₁₇	W18-1	TGW (g)	27.44 ± 0.21	27.46 ± 0.18	0.7023
		GL (mm)	8.323 ± 0.023	8.315 ± 0.025	0.2390
		GW (mm)	3.146 ± 0.027	3.149 ± 0.023	0.5819
	W18-2	TGW (g)	27.64 ± 0.34	28.17 ± 0.24	<0.0001	0.26	36.65
		GL (mm)	8.340 ± 0.036	8.352 ± 0.025	0.1796
		GW (mm)	3.140 ± 0.031	3.182 ± 0.025	<0.0001	0.021	25.49
	W18-3	TGW (g)	28.15 ± 0.18	28.19 ± 0.13	0.3866
		GL (mm)	8.373 ± 0.027	8.367 ± 0.023	0.4148
		GW (mm)	3.170 ± 0.016	3.169 ± 0.016	0.8698
TGW, 1000-grain weight; GL, Grain length; GW, Grain width; A, Additive effect of replacing a Zhenshan 97 allele with a Milyang 46 allele; R², Proportion of phenotypic variance explained by the QTL effect.

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Locus name	Gene product name
LOC_Os01g57120	Transposon protein, putative, unclassified
LOC_Os01g57130	Retrotransposon protein, putative, Ty3-gypsy subclass, expressed
LOC_Os01g57140	Hypothetical protein
LOC_Os01g57150	SR protein related family member, putative, expressed
LOC_Os01g57160	Transposon protein, putative, CACTA, En/Spm sub-class, expressed
LOC_Os01g57170	Expressed protein, uncharacterized
LOC_Os01g57180	Hypothetical protein
LOC_Os01g57190	Transposon protein, putative, unclassified, expressed
LOC_Os01g57210	Katanin p80 WD40 repeat-containing subunit B1 homolog 1, putative, expressed
LOC_Os01g57220	Secretory carrier-associated membrane protein, putative, expressed
LOC_Os01g57230	BTBN1 - Bric-a-Brac, Tramtrack, Broad Complex BTB domain with non-phototropic hypocotyl 3 NPH3 and coiled-coil domains, expressed
LOC_Os01g57240	Expressed protein, uncharacterized
LOC_Os01g57250	Expressed protein, uncharacterized