SB1 Encoding RING-Like Zinc-Finger Protein Regulates Branch Development as a Transcription Repressor

Xiaoqin Zeng, Hui Zhuang, Qinglan Cheng, Jun Tang, Fayu Yang, Mingjiang Huang, Ziyi Wang, Zhongcheng Li, Honghui Zhu, Rui Chen, Guanghua He, Yunfeng Li

Table S2. Primers used in the study.

Purpose Primer name Sequence Mapping SSR1-F CAGTGGCGTGGAGAGAAATTTGG SSR1-R CTCACCTGCGACAGCAAGATCG SSR2-F TGAGCACCATGCAATAACTGTCG SSR2-R ACCATCTATCGCCACATCTCACC SNP-1-F CGGGACATTCTTAGCTTTGCTTTGAT SNP-1-R GAAAGTAGTAGTCTCACCATGCTTTGCCAC SNP-2-F CCCACACTGACTGGGACTCACTCG SNP-2-R GATTAGCGTCTCGTCCTCGCAGC Candidate gene cloning SB1-F GCAATGGCACAAGTGACACTACAAGA SB1-R CTCCGATCAGTTCACGCAAAGTCG Complementation test SB1-com-F GTATTTGTCTGTGCTGGATCCGTGTGCGAAGGGATACATGATGAAGC SB1-com-R CGCCCTTGCTCACCATACTAGTCGGCCGCGGGTGGCC qPCR Actin-F TGCTATGTACGTCGCCATCCAG Actin-R AATGAGTAACCACGCTCCGTCA RT-SB1-F AACGCCACTACAATCTCTCACTCGC RT-SB1-R AGTCTAAAGGGTTCGCCTGCCTAA RT- MOC1-F CTGTGGTTGCATGACACAGATGCAC RT- MOC1-R CCTTGGATGGAATGTCTCAGTCCC RT- DEP1-F CCGTTTCTCGTTCTGGAT RT- DEP1-R ATCTGTGCCTCCTTCTCT RT-IPA1-F TCTTCTGTCAACCCAGCCAT RT-IPA1-R GCCATCAAAGCTGGTGGTAG RT-TAW1-F GACTGGAACACGTTCGGGCAGTAC RT-TAW1-R CACCTTGGTCTTGCCGAACTGGT RT-LAX1-F TGACGACGCTGGAGATGGCG RT-LAX1-R GGAGGCAGCTGATGAGCGCC RT-RCN11-F GACAGCAACCTGCTCAAGGTCATCTC RT-RCN1-R GCTGGTCGATTAGCTCCTTCACCC RT-OSH1-F CAGGACCTGGAGCTTCGCCAG RT-OSH1-R GGAGAGCGTGTTGAGCTGCGTCT RT-MOC3-F GGATCAGGCCAACGTCGTCAACT RT-MOC3-R CGACTGGGAAGAGTGGAAGCGTCT Two-Hybrid System SB1-BD-F1 ATGGGCAGCGGCGGCGGT SB1-BD-R1 GAACATCCCACGGTTTGGAGGTT SEU-AD-F1 ATGTCCAACCTCCAAACCGTGGG SEU-AD-R1- CTAAGATCCACAGATGGACATGGAGAG LEU-AD-F1 ATGTCGTCGCTCAGCCGGGAG LEU-AD-R1 CTATCTTTGGATTTGGTCTGCAGCT BiFC assay SB1-YC-F CAATTACAGGTACCCGGGGATCCATGGCGGGGTTCCCTCTAGGC SB1-YC-R TGCACGCTGCCACCGCCGTCGACCGGCCGCGGGTGGCCGTGGAA OsLUG1-YN-F CATCGAGGACGCCGGCGGATCCATGGCGCAGAGCAACTGGGAAG OsLUG1-YN-R GAACGAAAGCTCTGCAGGTCGACCTTCCACAGCTTGACAGAGTTGTCG OsSEU1-YN-F CATCGAGGACGCCGGCGGATCCATGTCTGGGGCGCCATGCTC OsSEU1-YN-R GAACGAAAGCTCTGCAGGTCGACCATGTTCCATGAGTAGCCACCACCT ChIP-qPCR DEP1-P1-F CGCCCACACACAACACAGCTAG DEP1-P1-R GACCGGGAACACACGTTGTCA DEP1-P2-F AGCTCAACTGAACGCTGGCTG DEP1-P2-R CCACCACTGCTGCTACTGCCTA DEP1-P3-F ACGAAGGATCGGCTTTGCAT DEP1-P3-R CGAGCCTACGTTGGATACGCT DEP1-P4-F GGAGCTACCCGCTACTGCAAG DEP1-P4-R CGTGATTCACCTCCGCCTAGA DEP1-P5-F CCTGCATCATAACGTTCCTAGTGGT DEP1-P5-R CTCAGGACTGTGAGCTGTAATATGG DEP1-P6-F GCTGTAGTCCAGACTGCTGCTCATG DEP1-P6-R GTTGCACTGGGACTTGAAGCATG TAW1-P1-F TGGACCAGTTCGGCAAGACCAAG TAW1-P1-R TGCTCGCGGACCTCGCGGAGGTAG TAW1-P2-F TCGAGTCATGTCAGCACTGTGGC TAW1-P2-R GCATCACTCTCTTAACGCTGCTGC TAW1-P3-F TCTCTCTCTCTCTTGGACCACTGCG TAW1-P3-R CGAACTCCATCGTCGATCTGCAC TAW1-P4-F CGAGCAGGTACGAGTCGCAGAAG TAW1-P4-R CTTGGTCTTGCCGAACTGGTCC TAW1-P5-F ACTCCACTCCACTCCACTCCACTATG TAW1-P5-R TAGTAGACGAGACGACGTGCGAGAG TAW1-P6-F CTCTCTCTCTGCTGCTGCAACAAG TAW1-P6-R CTGCCTGACTGACTGACTCTCTCTG MOC1-P1-F GAGTGGCACTTGCTACTGTGCATG MOC1-P1-R TGATGATGGCCTCCAGATAACTTCC MOC1-P2-F TATGGCACAAACAGTCACAGCTGC MOC1-P2-R TGCTCATTCCTTGATGAGAGAGTGAG MOC1-P4-F GTGGAGAGCCGGAATCAACACTGT MOC1-P4-R GACATATAATGATACTCCGGCCATCTC MOC1-P5-F GCTGGGATTGCACAGTGAAGCTAG MOC1-P5-R GCACGTCCAACATCTGGGCTT MOC1-P6-F TACCTGGCGTTCAACCAGATCG MOC1-P6-R CGTCGAGGTCGAGGATGTGGAC IPA1-P1-F GAGTGGCACTTGCTACTGTGCATG IPA1-P1-R TGATGATGGCCTCCAGATAACTTCC IPA1-P2-F TATGGCACAAACAGTCACAGCTGC IPA1-P2-R TGCTCATTCCTTGATGAGAGAGTGAG IPA1-P3-F CCAGTTACAACCCTCCACCATTCAC IPA1-P3-R TGTGGCAGTGCCCACAGTGTGT IPA1-P6-F GACGTGGCGTGTGGCAATGTAG IPA1-P6-R TACTCCGACGAGCCTCCTTTCTCT