
Rice Science ›› 2015, Vol. 22 ›› Issue (2): 53-64.DOI: 10.1016/S1672-6308(14)60285-7
• • 下一篇
收稿日期:2014-08-28
接受日期:2014-12-29
出版日期:2015-03-10
发布日期:2015-01-27
. [J]. Rice Science, 2015, 22(2): 53-64.
| Code | Accession No. | Local name | Region | Type |
|---|---|---|---|---|
| PSM1 | 807 | Paw San Bay Kyar | Aeyawady | Indica |
| PSM2 | 870 | Mee Don | Aeyawady | Japonica |
| PSM3 | 923 | NgaKywe | Yangon | Japonica |
| PSM4 | 930 | Paw San Hmwe | Pago | Indica |
| PSM5 | 962 | Paw San Hmwe | Pago | Indica |
| PSM6 | 998 | Mee Don Saba | Kayin | Indica |
| PSM7 | 1 045 | ZawGyiPyan (Mee don) | Aeyawady | Indica |
| PSM8 | 1 105 | NgaKywe (U To) | Aeyawady | Japonica |
| PSM9 | 1 139 | Paw San Hmwe | Pago | Indica |
| PSM10 | 1 207 | Paw San Hmwe | Aeyawady | Japonica |
| PSM11 | 1 495 | Paw San Hmwe | Yangon | Indica |
| PSM12 | 1 627 | Paw San Hmwe | Mon | Japonica |
| PSM13 | 1 641 | Paw San Hmwe | Aeyawady | Japonica |
| PSM14 | 1 981 | NgaKywe | Yangon | Indica |
| PSM15 | 2 117 | Mee Don Hmwe | Aeyawady | Indica |
| PSM16 | 2 176 | Hnan War Mee Don | Rakhine | Indica |
| PSM17 | 2 447 | NgaKywe | Pago | Indica |
| PSM18 | 2 460 | Paw San Bay Kyar | Aeyawady | Indica |
| PSM19 | 2 499 | Paw San Hmwe | Pago | Indica |
| PSM20 | 2 500 | Paw San Hmwe | Pago | Japonica |
| PSM21 | 2 501 | Paw San Hmwe | Yangon | Japonica |
| PSM22 | 2 502 | Paw San Hmwe | Aeyawady | Japonica |
| PSM23 | 2 522 | Paw San Hmwe | Aeyawady | Japonica |
| PSM24 | 2 578 | Paw San Shwe War | Aeyawady | Indica |
| PSM25 | 2 620 | Paw San Hmwe | Aeyawady | Indica |
| PSM26 | 2 878 | Hnan War Mee Don | Sagaing | Indica |
| PSM27 | 2 946 | Paw San Hmwe | Pago | Indica |
| PSM28 | 3 225 | Mee Don Yoe Sein | Aeyawady | Indica |
| PSM29 | 3 530 | Bay Kyar Lay | Aeyawady | Japonica |
| PSM30 | 5 802 | Paw San Hmwe | Chin | Japonica |
| PSM31 | 11 800 | Paw San Hmwe | Aeyawady | Indica |
Table 1 Information of Paw San Hmwe (PSM) accessions used in this study.
| Code | Accession No. | Local name | Region | Type |
|---|---|---|---|---|
| PSM1 | 807 | Paw San Bay Kyar | Aeyawady | Indica |
| PSM2 | 870 | Mee Don | Aeyawady | Japonica |
| PSM3 | 923 | NgaKywe | Yangon | Japonica |
| PSM4 | 930 | Paw San Hmwe | Pago | Indica |
| PSM5 | 962 | Paw San Hmwe | Pago | Indica |
| PSM6 | 998 | Mee Don Saba | Kayin | Indica |
| PSM7 | 1 045 | ZawGyiPyan (Mee don) | Aeyawady | Indica |
| PSM8 | 1 105 | NgaKywe (U To) | Aeyawady | Japonica |
| PSM9 | 1 139 | Paw San Hmwe | Pago | Indica |
| PSM10 | 1 207 | Paw San Hmwe | Aeyawady | Japonica |
| PSM11 | 1 495 | Paw San Hmwe | Yangon | Indica |
| PSM12 | 1 627 | Paw San Hmwe | Mon | Japonica |
| PSM13 | 1 641 | Paw San Hmwe | Aeyawady | Japonica |
| PSM14 | 1 981 | NgaKywe | Yangon | Indica |
| PSM15 | 2 117 | Mee Don Hmwe | Aeyawady | Indica |
| PSM16 | 2 176 | Hnan War Mee Don | Rakhine | Indica |
| PSM17 | 2 447 | NgaKywe | Pago | Indica |
| PSM18 | 2 460 | Paw San Bay Kyar | Aeyawady | Indica |
| PSM19 | 2 499 | Paw San Hmwe | Pago | Indica |
| PSM20 | 2 500 | Paw San Hmwe | Pago | Japonica |
| PSM21 | 2 501 | Paw San Hmwe | Yangon | Japonica |
| PSM22 | 2 502 | Paw San Hmwe | Aeyawady | Japonica |
| PSM23 | 2 522 | Paw San Hmwe | Aeyawady | Japonica |
| PSM24 | 2 578 | Paw San Shwe War | Aeyawady | Indica |
| PSM25 | 2 620 | Paw San Hmwe | Aeyawady | Indica |
| PSM26 | 2 878 | Hnan War Mee Don | Sagaing | Indica |
| PSM27 | 2 946 | Paw San Hmwe | Pago | Indica |
| PSM28 | 3 225 | Mee Don Yoe Sein | Aeyawady | Indica |
| PSM29 | 3 530 | Bay Kyar Lay | Aeyawady | Japonica |
| PSM30 | 5 802 | Paw San Hmwe | Chin | Japonica |
| PSM31 | 11 800 | Paw San Hmwe | Aeyawady | Indica |
| Trait | Gene | Chromosome | Marker | Reference |
|---|---|---|---|---|
| Amylose content | Wx | 6 | Waxy (G-T) | Ayres et al, 1997 |
| 6 | Waxy (CT)n | Bligh et al, 1995 | ||
| Fragrance | Os2AP | 8 | Aromarker | Vanavichit et al, 2008 |
| 8 | 3In2AP | Myint et al, 2012a | ||
| 8 | FMbadh2-E2A | Shi et al, 2008 | ||
| Gelatinization temperature | SSIIa | 6 | SNP2340-41 | Katengam et al, 2008 |
| 6 | SNP2209 | Katengam et al, 2008 |
Table 2 Gene specific markers for grain qualities used in clustering 31 Paw San Hmwe accessions.
| Trait | Gene | Chromosome | Marker | Reference |
|---|---|---|---|---|
| Amylose content | Wx | 6 | Waxy (G-T) | Ayres et al, 1997 |
| 6 | Waxy (CT)n | Bligh et al, 1995 | ||
| Fragrance | Os2AP | 8 | Aromarker | Vanavichit et al, 2008 |
| 8 | 3In2AP | Myint et al, 2012a | ||
| 8 | FMbadh2-E2A | Shi et al, 2008 | ||
| Gelatinization temperature | SSIIa | 6 | SNP2340-41 | Katengam et al, 2008 |
| 6 | SNP2209 | Katengam et al, 2008 |
| Code | Waxy (G-T) | Waxy (CT)n | SNP2340-41 | SNP2209 | 3In2AP | FMbadh2-E2A | Aromarker |
|---|---|---|---|---|---|---|---|
| PSM1 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM2 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM3 | T | 11月19日 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM4 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM5 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM6 | G | 11 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM7 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM8 | G | 19 | High GT | High GT | Non aroma (197/194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM9 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM10 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM11 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM12 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM13 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM14 | G/T | 11月17日 | High/low GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM15 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM16 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM17 | G | 17 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM18 | G/T | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM19 | G | 11月19日 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM20 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM21 | T | 11月19日 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM22 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM23 | G | 19 | High GT | High GT | Non aroma (197/194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM24 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM25 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM26 | G | 11月17日 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM27 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM28 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM29 | G/T | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM30 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM31 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| KDML105 | T | 17 | Low GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Aroma (392 bp) |
| Basmati 370 | G | 17 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Aroma (392 bp) |
| IR57514 | G | 11 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
Table 4 Thirty one Paw San Hmwe (PSM) accessions and controls assayed with seven rice quality markers.
| Code | Waxy (G-T) | Waxy (CT)n | SNP2340-41 | SNP2209 | 3In2AP | FMbadh2-E2A | Aromarker |
|---|---|---|---|---|---|---|---|
| PSM1 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM2 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM3 | T | 11月19日 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM4 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM5 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM6 | G | 11 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM7 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM8 | G | 19 | High GT | High GT | Non aroma (197/194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM9 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM10 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM11 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM12 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM13 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM14 | G/T | 11月17日 | High/low GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM15 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM16 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM17 | G | 17 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM18 | G/T | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM19 | G | 11月19日 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM20 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM21 | T | 11月19日 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM22 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM23 | G | 19 | High GT | High GT | Non aroma (197/194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM24 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM25 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM26 | G | 11月17日 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM27 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM28 | G | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM29 | G/T | 19 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM30 | G | 19 | High GT | High GT | Aroma (197 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| PSM31 | G | 19 | High GT | High/low GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
| KDML105 | T | 17 | Low GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Aroma (392 bp) |
| Basmati 370 | G | 17 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Aroma (392 bp) |
| IR57514 | G | 11 | High GT | High GT | Non aroma (194 bp) | Non aroma (207 bp) | Non aroma (400 bp) |
Fig. 2. Dendrogram of 31 Paw San Hmwe (PSM) accessions (blue), reference checks (violet) and standard checks (red) generated by cluster analysis using unweighted neighbor-joining of DARwin software based on 7 rice quality markers and 19 SSR markers. G1, G2, G5A, G5B and G6 represent the cluster groups; SG1, SG2 and SG3 represent the sub-group in G5B based on the genotype and phenotype of the PSM accessions.
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