Phenotype of Rice Floury Endosperm Mutant flo7 and Fine Mapping of Mutated Gene

doi:10.1016/S1672-6308(14)60299-7

摘要/Abstract

. [J]. Rice Science, 2015, 22(4): 162-170.

图/表 11

参考文献 36

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36	(Managing Editor: Li Guan)

Primer	Forward (5'-3')	Reverse (5'-3')	Product size (bp)
RM6411	GGGTATTGTCGGTGTTCAGG	GAAGGCTGTACCATCCAACG	140
RM1227	CATGGTAGCACACACCCTTG	CATCGACATGTGGACCACTC	137
RM28746	GAAGAAAGAAGACGCCAAGAAACG	CATTCCATTCCCTTCCTCTTCG	158
C5-2	GCTGTCAGATCGGTAGGCA	ACCACATCAACCCAGAAAA	90
C5-15	TGTTTGTGCCTTCAGATAA	TGAGATAGGGGAAGTAGAT	87
C2-6	AGATTCTTTTCCAAACAAC	AATCATCATATTGCCGAAA	81
C2-11	TGCATCATCCAATCTGTCTTGG	TCGACAAGCCAATCTACTCTTCC	139
C7-3	CTACGTGCATCCATCGAGAA	TGAATTGTTTACCGGAACCC	153
C7-5	TCTTCTACTCCTCGTCTTCGTTCG	CATGCAGAGCAGAGACTTCTTGG	143
C7-12	TGCTGGACACAGCAAGTTTT	TAGATGGTGGGGGTGATAGG	185
C7-15	GCGTTGATTGATGATGATGG	CGACCCGACGTAAAAGAGAA	113

Primer	Forward (5'-3')	Reverse (5'-3')	Product size (bp)
RM6411	GGGTATTGTCGGTGTTCAGG	GAAGGCTGTACCATCCAACG	140
RM1227	CATGGTAGCACACACCCTTG	CATCGACATGTGGACCACTC	137
RM28746	GAAGAAAGAAGACGCCAAGAAACG	CATTCCATTCCCTTCCTCTTCG	158
C5-2	GCTGTCAGATCGGTAGGCA	ACCACATCAACCCAGAAAA	90
C5-15	TGTTTGTGCCTTCAGATAA	TGAGATAGGGGAAGTAGAT	87
C2-6	AGATTCTTTTCCAAACAAC	AATCATCATATTGCCGAAA	81
C2-11	TGCATCATCCAATCTGTCTTGG	TCGACAAGCCAATCTACTCTTCC	139
C7-3	CTACGTGCATCCATCGAGAA	TGAATTGTTTACCGGAACCC	153
C7-5	TCTTCTACTCCTCGTCTTCGTTCG	CATGCAGAGCAGAGACTTCTTGG	143
C7-12	TGCTGGACACAGCAAGTTTT	TAGATGGTGGGGGTGATAGG	185
C7-15	GCGTTGATTGATGATGATGG	CGACCCGACGTAAAAGAGAA	113

Primer	Accession number	Forward (5'-3')	Reverse (5'-3')
Actin	X16280	CATGCTATCCCTCGTCTCGACCT	CGCACTTCATGATGGAGTTGTAT
OsAGPS1	AK073146	AGAATGCTCGTATTGGAGAAAATG	GGCAGCATGGAATAAACCAC
OsAGPS2a	AK071826	ACTCCAAGAGCTCGCAGACC	GCCTGTAGTTGGCACCCAGA
OsAGPS2b	AK103906	AACAATCGAAGCGCGAGAAA	GCCTGTAGTTGGCACCCAGA
OsAGPL1	D50317	GGAAGACGGATGATCGAGAAAG	CACATGAGATGCACCAACGA
OsAGPL3	AK069296	AAGCCAGCCATGACCATTTG	CACACGGTAGATTCACGAGACAA
OsAGPL4	AK121036	TCAACGTCGATGCAGCAAAT	ATCCCTCAGTTCCTAGCCTCATT
OsSSI	D16202	GGGCCTTCATGGATCAACC	CCGCTTCAAGCATCCTCATC
OsSSIIa	AF419099	GCTTCCGGTTTGTGTGTTCA	CTTAATACTCCCTCAACTCCACCAT
OsSSIIIa	AY100469	GCCTGCCCTGGACTACATTG	GCAAACATATGTACACGGTTCTGG
OsBEIIb	D16201	ATGCTAGAGTTTGACCGC	AGTGTGATGGATCCTGCC
GBSSI	AK070431	TCCGAGAGGTTCAGGTCATC	ATGAGCTCCTCGGCGTAGTA
OsISA1	AB093426	TGCTCAGCTACTCCTCCATCATC	AGGACCGCACAACTTCAACATA
OsISA2	AC132483	TAGAGGTCCTCTTGGAGG	AATCAGCTTCTGAGTCACCG
OsISA3	AP005574	ACAGCTTGAGACACTGGGTTGAG	GCATCAAGAGGACAACCATCTG
OsPUL	AB012915	ACCTTTCTTCCATGCTGG	CAAAGGTCTGAAAGATGGG
OsPHOL	AK063766	TTGGCAGGAAGGTTTCGCT	CGAAGCCTGAAGTGAACTTGCT

Primer	Accession number	Forward (5'-3')	Reverse (5'-3')
Actin	X16280	CATGCTATCCCTCGTCTCGACCT	CGCACTTCATGATGGAGTTGTAT
OsAGPS1	AK073146	AGAATGCTCGTATTGGAGAAAATG	GGCAGCATGGAATAAACCAC
OsAGPS2a	AK071826	ACTCCAAGAGCTCGCAGACC	GCCTGTAGTTGGCACCCAGA
OsAGPS2b	AK103906	AACAATCGAAGCGCGAGAAA	GCCTGTAGTTGGCACCCAGA
OsAGPL1	D50317	GGAAGACGGATGATCGAGAAAG	CACATGAGATGCACCAACGA
OsAGPL3	AK069296	AAGCCAGCCATGACCATTTG	CACACGGTAGATTCACGAGACAA
OsAGPL4	AK121036	TCAACGTCGATGCAGCAAAT	ATCCCTCAGTTCCTAGCCTCATT
OsSSI	D16202	GGGCCTTCATGGATCAACC	CCGCTTCAAGCATCCTCATC
OsSSIIa	AF419099	GCTTCCGGTTTGTGTGTTCA	CTTAATACTCCCTCAACTCCACCAT
OsSSIIIa	AY100469	GCCTGCCCTGGACTACATTG	GCAAACATATGTACACGGTTCTGG
OsBEIIb	D16201	ATGCTAGAGTTTGACCGC	AGTGTGATGGATCCTGCC
GBSSI	AK070431	TCCGAGAGGTTCAGGTCATC	ATGAGCTCCTCGGCGTAGTA
OsISA1	AB093426	TGCTCAGCTACTCCTCCATCATC	AGGACCGCACAACTTCAACATA
OsISA2	AC132483	TAGAGGTCCTCTTGGAGG	AATCAGCTTCTGAGTCACCG
OsISA3	AP005574	ACAGCTTGAGACACTGGGTTGAG	GCATCAAGAGGACAACCATCTG
OsPUL	AB012915	ACCTTTCTTCCATGCTGG	CAAAGGTCTGAAAGATGGG
OsPHOL	AK063766	TTGGCAGGAAGGTTTCGCT	CGAAGCCTGAAGTGAACTTGCT

Material	Plant height (cm)	Main panicle length (cm)	Effective tiller number per plant	No. of grains per panicle	Seed-setting rate (%)
WT	67.25 ± 4.25	16.88 ± 2.32	26.25 ± 2.75	60.00 ± 5.80	66.25 ± 8.32
flo7	74.20 ± 3.50	17.22 ± 1.80	26.75 ± 4.68	61.75 ± 5.25	82.20 ± 9.33
P value (t test)	0.003	0.190	0.150	0.850	0.036

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Metrics

Material	Protein content (%)	Amylose content (%)	Gel consistency (mm)	Alkali spreading value	Hardness (N)
WT	10.2 ± 0.2	17.9 ± 0.2	80.5 ± 0.5	6.0 ± 0.5	19.5
flo7	10.7 ± 0.2	15.4 ± 0.3	56.6 ± 0.5	6.8 ± 0.2	15.4
P value (t test)	0.180	0.012	0.002	0.035	0.018

Combination	No. of wild type plants	No. of flo7 type plants	χ²_{(3:1 )}	P value
flo7/Nanjing 11	502	179	0.600	0.44
flo7/Pei’ai 64	89	28	0.025	0.79
flo7/93-11	102	36	0.038	0.77

ORF	Candidate gene	Function description
ORF1	Os12g0630750	Full insert sequence
ORF2	Os12g0630800	Hypothetical conserved gene
ORF3	Os12g0631025	Non-protein coding transcript
ORF4	Os12g0631100	Small GTPase, storage protein trafficking
ORF5	Os12g0631150	Non-protein coding transcript
ORF6	Os12g0631200	Similar to ubiquitin-protein ligase / zinc ion binding protein
ORF7	Os12g0631600	Conserved hypothetical protein.
ORF8	Os12g0631800	Phytoene dehydrogenase-like
ORF9	Os12g0632000	Similar to glycine-rich RNA-binding protein 1
ORF10	Os12g0632100	Similar to ARL2 G-protein
ORF11	Os12g0632401	Hypothetical conserved gene
ORF12	Os12g0632600	Similar to helix-loop-helix DNA-binding domain containing protein
ORF13	Os12g0632700	Malate dehydrogenase, glyoxysomal precursor