Rice Science ›› 2016, Vol. 23 ›› Issue (1): 1-8.DOI: 10.1016/j.rsci.2016.01.001
• • 下一篇
收稿日期:2015-05-12
接受日期:2015-07-06
出版日期:2016-01-20
发布日期:2015-11-05
. [J]. Rice Science, 2016, 23(1): 1-8.
| Marker | Forward primer (5'→3') | Reverse primer (5'→3') |
| RM23804 | GAGCCGACTCAATCCAATCCTCTCC | ATCGTCTTCCAAGCAAGTGCAAGC |
| RM23893 | GGCGGCTTAAGAGTGTTTGTAGG | AGAGGATTGCTTTGCTGATGAGG |
| RM23904 | CTCACCGGAGCACCACTAACC | GAGAGCAAGACTGTGAAGTGTGAACC |
| RM7038 | GATTAGAGCTTTGGTGGTTCTTGG | ACTTGTGGTCGGTCTGGTAGTCC |
| InDel_6 | GAATCCGGTTCCGAGACTAAA | TCAATCTTGCAGCAAACAAGGC |
| InDel_10 | ACCTGATCGATGTCTAATTTGCAC | CGTTTACGAAAGGCAGAGGACA |
Table 1 Molecular markers used for gene mapping.
| Marker | Forward primer (5'→3') | Reverse primer (5'→3') |
| RM23804 | GAGCCGACTCAATCCAATCCTCTCC | ATCGTCTTCCAAGCAAGTGCAAGC |
| RM23893 | GGCGGCTTAAGAGTGTTTGTAGG | AGAGGATTGCTTTGCTGATGAGG |
| RM23904 | CTCACCGGAGCACCACTAACC | GAGAGCAAGACTGTGAAGTGTGAACC |
| RM7038 | GATTAGAGCTTTGGTGGTTCTTGG | ACTTGTGGTCGGTCTGGTAGTCC |
| InDel_6 | GAATCCGGTTCCGAGACTAAA | TCAATCTTGCAGCAAACAAGGC |
| InDel_10 | ACCTGATCGATGTCTAATTTGCAC | CGTTTACGAAAGGCAGAGGACA |
Fig. 1. Phenotypes of the mutant bh6 and its wild type IR64.
| Plant height | Panicle length | No. of productive panicles per plant | No. of filled grains | |
| (cm) | (cm) | per panicle | ||
| 120.0 ± 1.5 | 27.4 ± 0.3 | 15 ± 3 | 89 ± 12 | |
| 119.4 ± 1.4 | 26.1 ± 0.4* | 16 ± 2 | 81 ± 16 | |
| * and ** represent significant difference at the 0.05 and 0.01 levels, respectively. | ||||
Table 2 Performance of agronomic traits between wild type IR64 and mutant bh6.
| Plant height | Panicle length | No. of productive panicles per plant | No. of filled grains | |
| (cm) | (cm) | per panicle | ||
| 120.0 ± 1.5 | 27.4 ± 0.3 | 15 ± 3 | 89 ± 12 | |
| 119.4 ± 1.4 | 26.1 ± 0.4* | 16 ± 2 | 81 ± 16 | |
| * and ** represent significant difference at the 0.05 and 0.01 levels, respectively. | ||||
Fig. 2. Total contents of flavonoid and anthocyanin in rice hulls.
| Cross combination | F1 | F2 | P(3:1) | ||
| Total No. of plants | No. of wild type plants | No. of mutant type plants | |||
| bh6/CPSLO17 | Normal | 2 107 | 1 600 | 507 | 0.32 |
| bh6/IR64 | Normal | 684 | 507 | 177 | 0.6 |
Table 3 Genetic analysis of brown hull mutant bh6.
| Cross combination | F1 | F2 | P(3:1) | ||
| Total No. of plants | No. of wild type plants | No. of mutant type plants | |||
| bh6/CPSLO17 | Normal | 2 107 | 1 600 | 507 | 0.32 |
| bh6/IR64 | Normal | 684 | 507 | 177 | 0.6 |
| F3 line | Total No. of plants | No. of wild type plants | No. of mutant | P(3:1) |
| type plants | ||||
| Line 1 | 276 | 216 | 60 | 0.21 |
| Line 2 | 212 | 163 | 49 | 0.53 |
| Line 3 | 132 | 107 | 25 | 0.11 |
Table 4 Genetic analysis of F3 segregation lines derived from bh6/ IR64.
| F3 line | Total No. of plants | No. of wild type plants | No. of mutant | P(3:1) |
| type plants | ||||
| Line 1 | 276 | 216 | 60 | 0.21 |
| Line 2 | 212 | 163 | 49 | 0.53 |
| Line 3 | 132 | 107 | 25 | 0.11 |
Fig. 3. Fine mapping of the mutation on chromosome 9.A, bh6 is linked with SSR markers RM23804, RM23893, RM23904 and RM7038; B, bh6 is located in a 106 kb region between InDel_6 and InDel_10; C, Single base substitution at position 1013 (G1013A).
Fig. 4. Functional complementation of the mutation.A, Complementation construct p1300-9.3; B, Phenotype of IR64, bh6 and complementary transgenic line (c-bh6); C, Sequencing analysis of complementary transgenic lines. The arrow refers to the mutation site.
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