Rice Science ›› 2023, Vol. 30 ›› Issue (1): 6-10.DOI: 10.1016/j.rsci.2022.03.003
收稿日期:
2022-01-17
接受日期:
2022-03-30
出版日期:
2023-01-28
发布日期:
2022-11-11
. [J]. Rice Science, 2023, 30(1): 6-10.
Plant | Extraction solvent | Average inhibition rate (%) | Remark |
Negative control | Ethanol (10%) | 10 ± 2 | Most spores germinated and produced long germ tubes (Fig. S1-A) |
Positive control | Isoprothiolane (20 μg/mL) | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-B) |
Paris polyphylla | Ethanol | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-C) |
Seed | 52 ± 4** | ||
Stem | 43 ± 5** | ||
Leaf | 51 ± 5** | ||
Pericarp | 77 ± 7** | ||
Root | 78 ± 8** | ||
Swertia bimaculate | n-butanol | 15 ± 2 | Most spores germinated and produced long germ tubes |
Petroleum ether | 15 ± 2 | ||
Ethyl acetate | 15 ± 2 | ||
Menispermum dauricum | Ethyl acetate | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
Water | 20 ± 2** | ||
n-butanol | 50 ± 5** | Spore germination was highly inhibited (Fig. S1-D) | |
Nelumbo nucifera | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
Petroleum ether | 20 ± 3* | ||
Chloroform | 20 ± 3* | ||
Phellodendron chinense | Ethyl acetate | 70 ± 5** | Spore germination was mostly inhibited |
Water | 10 ± 2 | Most spores germinated and produced long germ tubes | |
Carthamus tinctorius | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
Water | 20 ± 3* | ||
Taraxacum mongolicum | Petroleum ether | 20 ± 4* | Low inhibition rates; long germ tubes were observed |
Water | 20 ± 4* | ||
Momordica charantia | Petroleum ether | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
n-butanol | 20 ± 2** | ||
Ethyl acetate | 20 ± 2** | ||
Chloroform | 30 ± 2** | Moderate inhibition rate; long germ tubes were observed | |
Twenty extracts with ≥ 10% inhibition rates are listed. Data are Mean ± SD. * and **, Statistically significant at P < 0.05 and P < 0.01, respectively, in Dunnett’s multiple comparison test with the negative control (10% ethanol). |
Table 1. Inhibitory effects of 20 plant extracts and ethanol extracts from different parts of Paris polyphylla on Magnaporthe oryzae sporegermination.
Plant | Extraction solvent | Average inhibition rate (%) | Remark |
Negative control | Ethanol (10%) | 10 ± 2 | Most spores germinated and produced long germ tubes (Fig. S1-A) |
Positive control | Isoprothiolane (20 μg/mL) | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-B) |
Paris polyphylla | Ethanol | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-C) |
Seed | 52 ± 4** | ||
Stem | 43 ± 5** | ||
Leaf | 51 ± 5** | ||
Pericarp | 77 ± 7** | ||
Root | 78 ± 8** | ||
Swertia bimaculate | n-butanol | 15 ± 2 | Most spores germinated and produced long germ tubes |
Petroleum ether | 15 ± 2 | ||
Ethyl acetate | 15 ± 2 | ||
Menispermum dauricum | Ethyl acetate | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
Water | 20 ± 2** | ||
n-butanol | 50 ± 5** | Spore germination was highly inhibited (Fig. S1-D) | |
Nelumbo nucifera | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
Petroleum ether | 20 ± 3* | ||
Chloroform | 20 ± 3* | ||
Phellodendron chinense | Ethyl acetate | 70 ± 5** | Spore germination was mostly inhibited |
Water | 10 ± 2 | Most spores germinated and produced long germ tubes | |
Carthamus tinctorius | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
Water | 20 ± 3* | ||
Taraxacum mongolicum | Petroleum ether | 20 ± 4* | Low inhibition rates; long germ tubes were observed |
Water | 20 ± 4* | ||
Momordica charantia | Petroleum ether | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
n-butanol | 20 ± 2** | ||
Ethyl acetate | 20 ± 2** | ||
Chloroform | 30 ± 2** | Moderate inhibition rate; long germ tubes were observed | |
Twenty extracts with ≥ 10% inhibition rates are listed. Data are Mean ± SD. * and **, Statistically significant at P < 0.05 and P < 0.01, respectively, in Dunnett’s multiple comparison test with the negative control (10% ethanol). |
Fig. 1. Antifungal and resistance-inducing activities against rice blast fungus Magnaporthe oryzae. A, Increase in blast resistance of rice seedlings by application of Paris polyphylla pericarp extract. Four-leaf stage rice seedlings were foliar sprayed with P. polyphylla pericarp extract, followed by blast inoculation at 24 h later. Relative fungal growth of M. oryzae was examined after 7 d of blast inoculation. B, Increase in expression levels of rice defense genes (WRKY45, PR1b and PBZ1) by P. polyphylla pericarp extract. Gene expression was examined after 3 d of blast inoculation. Data are Mean ± SD (n = 12). * indicates significant difference from the mock-treated plants (t-test, P < 0.05).
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