Rice Science ›› 2022, Vol. 29 ›› Issue (5): 402-406.DOI: 10.1016/j.rsci.2022.07.002
收稿日期:
2021-12-18
接受日期:
2022-02-15
出版日期:
2022-09-28
发布日期:
2022-07-04
. [J]. Rice Science, 2022, 29(5): 402-406.
Fig. 1. Methyl viologen (MV) induced oxidative stress of rice seedlings under high light. A?C, Root (A) and shoot (B) lengths, and total chlorophyll content (C) of rice seedlings at different MV concen- trations. Ten seedlings with three replications were used for each con- centration. One way ANOVA was used to assess the statistical significance. Lowercase letters above the bars indicate significant differences at the 0.01 level. D and E, Membrane integrity measured by Evan’s blue staining (D) and levels of singlet O2·? by nitro blue tetrazolium chloride (NBT) staining (E). Scale bars, 25 μm. Seven-day-old rice seedlings were exposed to MV oxidative stress at different concentrations under high light conditions [600 µmol/(m2∙s)] for 16 h. T1 to T5 refer to treatments with MV concentrations of 2.5, 5.0, 10.0, 15.0 and 20.0 μmol/L, respectively.
Fig. 2. Responses of rice seedlings treated with pronetalol (PRO) and methyl viologen (MV), and expression of OsDREB2A transcription factor target genes. A, Seedling growth at different PRO and MV concentrations. Scale bar, 1 cm. B?D, Shoot (B) and root (C) growth at different PRO and MV concentrations, and reduction in total chlorophyll content (D) over control. Ten seedlings with three replications were used for each concentration. E and F, Microscopic observation was taken for membrane integrity by Evan’s blue staining (E), and for levels of O2·? by nitro blue tetrazolium chloride (NBT) staining (F). Oxidative stress was imposed by MV at 20.0 µmol/L along with PRO (25.0, 50.0 and 75.0 µmol/L) for 10-day-old rice seedlings under high light conditions. Scale bars, 25 μm. G, Model depicting OsDREB2A binding to cis-elements of the target gene and small molecule (SM) interferences with cis-elements of target genes. H, Expression of OsDREB2A target genes HSFA3 and LEA7 by qRT-PCR. Minimum three replicates were used for each sample, and rice housekeeping gene ubiquitin was used for normalization. One-way ANOVA was used to assess the statistical significance. Different lowercase letters above the bars indicate significant differences at the 0.01 level. T1, 20.0 μmol/L MV; T2, 25.0 μmol/L PRO + 20.0 μmol/L MV; T3, 50.0 μmol/L PRO + 20.0 μmol/L MV; T4, 75.0 μmol/L PRO + 20.0 μmol/L MV; T5, 25.0 μmol/L PRO; T6, 50.0 μmol/L PRO; T7, 75.0 μmol/L PRO.
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