Efficient Breeding of Early-Maturing Rice Cultivar by Editing Hd6 via CRISPR/Cas9

doi:10.1016/j.rsci.2024.06.007

摘要/Abstract

. [J]. Rice Science, 2024, 31(6): 629-633.

Fig. 1. Knockout of Hd6 by CRISPR/Cas9 method produces early heading phenotype. A, Gene structure of Hd6. The white boxes represent the untranslated regions, black boxes stand for the coding regions, and black lines represent the introns. B, DNA sequences flanking the two target sites of sgRNAs in Nanjing 46 (NJ46) and its three mutants (NJ46-1, NJ46-2, and NJ46-3). Inserted sequences are shown in red letter(s), deleted sequences are highlighted by red lines, and blue nucleotides indicate PAM (protospacer adjacent motif). On the right of the numbers, ‘-’ means deletion, and ‘+’ means insertion/substitution. WT, Wild type. C, Phenotypes of NJ46 and three mutants at the heading stage. Scale bars, 5 cm. D, Flowering time of NJ46 and three mutants under long-day conditions at Nanjing city, Jiangsu Province, China, in two years. E, qRT-PCR analysis of the transcription levels of Hd3a and RFT1 in NJ46 and three mutant lines. RNA was extracted from 30-day-old leaves. The rice ubiquitin (UBQ) gene was used as an internal control. Data are Mean ± SD (n = 3). ** represents significant differences at P ≤ 0.01.

Fig. 2. Agronomic traits of Nanjing 46 (NJ46) and its three mutants (NJ46-1, NJ46-2, and NJ46-3). A, Measurements of five agronomic traits in NJ46 and three mutants. Data are Mean ± SD (n = 5). * and ** represent significant differences at P ≤ 0.05 and P ≤ 0.01, respectively. ns, Not significant. B, Morphology of NJ46 and three mutants at the maturity stage. Scale bars, 5 cm. C, Morphology of milled rice grains of NJ46 and three mutants. Scale bar, 2 cm. D, Comparison of rapid viscosity analyzer spectra of rice flours between NJ46 and three mutants.

参考文献 22

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