Fig. 1. Growth inhibition of Rhizoctonia solani GD118, circular genome map and phylogenetic analysis of Pseudomonas protegens JP2-4390, and production of pyrrolnitrin (PRN) from different media and mutant strains. A-D, Growth inhibition of R. solani GD118 by ddH2O (A), by JP2-4390 when GD118 was exposed to JP2-4390 diffusible and volatile compounds (B), by JP2-4390 when GD118 was solely exposed to JP2-4390 volatile compounds (C), and by JP2Δprn (D). E, Phylogenetic analysis of P. protegens JP2-4390 based on 16S rRNA gene sequence. The evolutionary distances were calculated using the Kimura 2-parameter method. The evolutionary history was inferred using the neighbor-joining method. The strain in this study is in bold type with red font. F, High performance liquid chromatography (HPLC) analysis of the pyrrolnitrin standard (a), JP2-4390 (b), and JP2Δprn fermentation crude extract (c). G, Production of PRN from different media. Glu represents sodium glutamate, YE represents yeast extract, and the values following ‘Glu’ and ‘YE’ show the contentration of each componet. H, Production of PRN from different mutant strains. In G and H, data represent Mean ± SD (n = 3). ** and ****, P < 0.01 and P < 0.0001, respectively, by two-tailed unpaired t test.