Fig. 1. Expression of OsPR10b and resistance of wild type Zhonghua 11 (ZH11) and mutants to rice blast and bacterial blight. A, Transcriptome sequencing analysis of rice samples at 0, 12, 24, and 48 h after Magnaporthe oryzae infection revealed that the expression level of OsPR10b was induced by M. oryzae (Guy11) infection. B, qRT-PCR analysis of two-week-old ZH11 rice leaves at 0, 12, 24, 48, and 72 h after M. oryzae infection revealed that the expression level of OsPR10b was elevated and peaked at 48 h post infection. Ubiquitin was used as an internal reference. C, Expression levels of OsPR10b in roots, shoots, and leaves of 2-, 4-, and 6-week-old seedlings, panicles of 0.5‒1.0, 1.1‒3.0, 3.1‒5.0, and 5.1‒10.0 cm in length, and germinating and mature seeds as well as calli. Ubiquitin was used as an internal reference. The expression level of OsPR10b was highest in calli. D, DNA sequencing of knockout mutants ospr10b-1 and ospr10b-2. E, Plants of ospr10b-1 and ospr10b-2 developed more diseased lesions than ZH11 plants after inoculation with the M. oryzae Guy11 isolate. Scale bar, 1 cm. F, Lesion number per cm2 on rice leaves after inoculation with the Guy11 isolate. G, Compared with wild type ZH11 plants, ospr10b-1 and ospr10b-2 mutant plants displayed increased susceptibility after inoculation with the Xanthomonas oryzae pv. oryzae PXO99 isolate. Scale bar, 1 cm. H, Statistical analysis of lesion length after inoculation with the PXO99 isolate. Data are presented as mean ± SE in A (n = 3), mean ± SEM in B (n = 3), and mean ± SD in C, F, and H (n = 3 in C, 12 in F, and 15 in H) using the Student’s t-test (**, P ≤ 0.01).