Fig. 1. Ustiloxins induce liver injury in mice under different ustiloxins concentrations. A, Relative expression levels of Gstm1, Gstm3, Htatip2, and Mcm7 in mouse liver after three-month oral administration of ustiloxins. B and C, Ustiloxin effect on relative expression levels of CYP450-related genes in mouse hepatocytes (BNLCL2 cell, B) and livers (C). D and E, Protein expression of CYP2E1 and CYP1B1 in mouse hepatocytes (BNLCL2 cell, D) and livers (E). F, Nrf2, HO-1, and Keap1 mRNA expression in mouse hepatocytes (BNLCL2 cell, left) and livers (right). G and H, Expression of several proteins associated with oxidative stress in mouse hepatocytes (BNLCL2 cell, G) and livers (H). I, IL-1β and IL-18 mRNA expression in mouse hepatocytes (left) and livers (right). J and K, Protein expression of Pro-IL-1β, cleaved-IL-1β, and IL-18 in mouse hepatocytes (BNLCL2 cell, J) and livers (K). In A, B, C, F, and I, GAPDH is the internal control. In D, E, G, H, J, and K, GAPDH is the internal control. In D, E, G, H, J, and K, we uniformly apply intra-group normalization to reflect only relative changes for each protein with the expression value under no ustiloxin treatments as the control ‘1’. Data are mean ± SD (n = 3). * and **, P < 0.05 and P < 0.01 by the Tukey’s honestly significant difference test.