Rice Science ›› 2015, Vol. 22 ›› Issue (2): 65-70.DOI: 10.1016/S1672-6308(14)60280-8
收稿日期:2014-09-16
接受日期:2014-10-27
出版日期:2015-03-10
发布日期:2015-01-27
. [J]. Rice Science, 2015, 22(2): 65-70.
Fig. 1. Sequences of the cloned GW2 cDNA in TD70 and its deduced amino acid sequences.The gray shadow indicates predicted open reading frame; The position of arrows points to one base deletion.
Fig. 2. Sequences of the GW2 coding protein domain function prediction in TD70 and Kasalath.
Fig. 3. PCR amplification of GW2 with its functional marker in TD70, Kasalath and parts of recombinant inbred lines (RILs). M, 50 bp Marker; T, TD70; K, Kasalath; Lanes 1-4, Parts of RILs.
| Type | Year | KGW (g) | GL (mm) | GW (mm) | GT (mm) |
|---|---|---|---|---|---|
| TD70 | 2012 | 65.00 ± 2.35 | 13.40 ± 0.08 | 4.42 ± 0.25 | 2.99 ± 0.05 |
| 2013 | 61.00 ± 2.04 | 13.29 ± 0.15 | 4.29 ± 0.05 | 2.93 ± 0.15 | |
| Kasalath | 2012 | 17.40 ± 0.66 | 8.04 ± 0.26 | 2.48 ± 0.07 | 1.84 ± 0.08 |
| 2013 | 17.80 ± 0.53 | 8.07 ± 0.09 | 2.48 ± 0.04 | 1.90 ± 0.01 | |
| RIL | 2012 | 31.20 ± 7.18 | 9.83 ± 1.24 | 3.12 ± 0.36 | 2.11 ± 0.16 |
| 2013 | 28.43 ± 6.46 | 9.68 ± 1.22 | 3.11 ± 0.37 | 2.17 ± 0.18 |
Table 1 Mean values of grain traits in recombinant inbred lines (RILs) and their parents in two years.
| Type | Year | KGW (g) | GL (mm) | GW (mm) | GT (mm) |
|---|---|---|---|---|---|
| TD70 | 2012 | 65.00 ± 2.35 | 13.40 ± 0.08 | 4.42 ± 0.25 | 2.99 ± 0.05 |
| 2013 | 61.00 ± 2.04 | 13.29 ± 0.15 | 4.29 ± 0.05 | 2.93 ± 0.15 | |
| Kasalath | 2012 | 17.40 ± 0.66 | 8.04 ± 0.26 | 2.48 ± 0.07 | 1.84 ± 0.08 |
| 2013 | 17.80 ± 0.53 | 8.07 ± 0.09 | 2.48 ± 0.04 | 1.90 ± 0.01 | |
| RIL | 2012 | 31.20 ± 7.18 | 9.83 ± 1.24 | 3.12 ± 0.36 | 2.11 ± 0.16 |
| 2013 | 28.43 ± 6.46 | 9.68 ± 1.22 | 3.11 ± 0.37 | 2.17 ± 0.18 |
| Genotype | Year | No. of RILs | KGW (g) | GL (mm) | GW (mm) | GT (mm) |
|---|---|---|---|---|---|---|
| GW2TD70 | 2012 | 82 | 35.18 ± 7.28 | 9.96 ± 1.26 | 3.43 ± 0.33 | 2.23 ± 0.14 |
| 2013 | 82 | 32.08 ± 6.16 | 9.82 ± 1.21 | 3.44 ± 0.33 | 2.32 ± 0.15 | |
| GW2Kasalath | 2012 | 158 | 29.13 ± 6.22 | 9.77 ± 1.23 | 2.96 ± 0.26 | 2.04 ± 0.12 |
| 2013 | 158 | 26.54 ± 5.78 | 9.61 ± 1.22 | 2.93 ± 0.25 | 2.10 ± 0.14 |
Table 2 Grain phenotype of lines with different GW2 genotype in recombinant inbred lines (RILs).
| Genotype | Year | No. of RILs | KGW (g) | GL (mm) | GW (mm) | GT (mm) |
|---|---|---|---|---|---|---|
| GW2TD70 | 2012 | 82 | 35.18 ± 7.28 | 9.96 ± 1.26 | 3.43 ± 0.33 | 2.23 ± 0.14 |
| 2013 | 82 | 32.08 ± 6.16 | 9.82 ± 1.21 | 3.44 ± 0.33 | 2.32 ± 0.15 | |
| GW2Kasalath | 2012 | 158 | 29.13 ± 6.22 | 9.77 ± 1.23 | 2.96 ± 0.26 | 2.04 ± 0.12 |
| 2013 | 158 | 26.54 ± 5.78 | 9.61 ± 1.22 | 2.93 ± 0.25 | 2.10 ± 0.14 |
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