Evaluation of Inherited Resistance Genes of Bacterial Leaf Blight, Blast and Drought Tolerance in Improved Rice Lines

doi:10.1016/j.rsci.2020.08.001

摘要/Abstract

. [J]. Rice Science, 2021, 28(3): 279-288.

图/表 9

参考文献 50

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Genotype	Blast resistance gene				BLB resistance gene					Drought tolerance QTL
	Pi9	Pi2	Piz		Xa4		xa5	xa13	Xa21	qDTY_2.2		qDTY_3.2	qDTY_12.1
	RM6836	RM6836	RM6836/ RM8225		RM224		RM122/ RM13	Xa13prom/ RG136	RM21/ pTA248	RM236		RM520	RM511/ RM1261
Putra-1 (P)^a	+	+		+		-	-	-	-		-	-	-
IRBB60 (B)^a	-	-		-		+	+	+	+		-	-	-
MR219-PL-137 (D)^a	-	-		-		-	-	-	-		+	+	+
PD14	+	+		+		-,,	-	-	-		+	+	+
PD15	+	+		+		-	-	-	-		+	+	+
PB12	+	+		+		+	+	+	+		-	-	-
PB14	+	+		+		+	+	+	+		-	-	-
PBD1	+	+		+		+	+	+	+		+	+	+
PBD3	+	+		+		+	+	+	+		+	+	+
PDB3	+	+		+		+	+	+	+		+	+	+
DPB7	+	+		+		+	+	+	+		+	+	+
DPB12	+	+		+		+	+	+	+		-	+	+
DPB13	+	+		+		+	+	+	+		+	+	+
DPB20	+	+		+		+	+	+	+		+	+	+

Genotype	Blast resistance gene				BLB resistance gene					Drought tolerance QTL
	Pi9	Pi2	Piz		Xa4		xa5	xa13	Xa21	qDTY_2.2		qDTY_3.2	qDTY_12.1
	RM6836	RM6836	RM6836/ RM8225		RM224		RM122/ RM13	Xa13prom/ RG136	RM21/ pTA248	RM236		RM520	RM511/ RM1261
Putra-1 (P)^a	+	+		+		-	-	-	-		-	-	-
IRBB60 (B)^a	-	-		-		+	+	+	+		-	-	-
MR219-PL-137 (D)^a	-	-		-		-	-	-	-		+	+	+
PD14	+	+		+		-,,	-	-	-		+	+	+
PD15	+	+		+		-	-	-	-		+	+	+
PB12	+	+		+		+	+	+	+		-	-	-
PB14	+	+		+		+	+	+	+		-	-	-
PBD1	+	+		+		+	+	+	+		+	+	+
PBD3	+	+		+		+	+	+	+		+	+	+
PDB3	+	+		+		+	+	+	+		+	+	+
DPB7	+	+		+		+	+	+	+		+	+	+
DPB12	+	+		+		+	+	+	+		-	+	+
DPB13	+	+		+		+	+	+	+		+	+	+
DPB20	+	+		+		+	+	+	+		+	+	+

Crossing method	Population code	Lines developed	Introgressed genotype
Single cross	PD	PD14, PD15	Putra-1 × MR219-PL-137
Single cross	PB	PB12, PB14	Putra-1 × IRBB60
Double cross	PDB	PDB3	Putra-1 × MR219-PL-137 × IRBB60
Three-way cross	PBD	PBD1, PBD3	Putra-1× IRBB60 × MR219-PL-137
Three-way reciprocal cross	DPB	DPB7, DPB12, DPB13, DPB20	MR219-PL-137 × Putra-1 × IRBB60

Crossing method	Population code	Lines developed	Introgressed genotype
Single cross	PD	PD14, PD15	Putra-1 × MR219-PL-137
Single cross	PB	PB12, PB14	Putra-1 × IRBB60
Double cross	PDB	PDB3	Putra-1 × MR219-PL-137 × IRBB60
Three-way cross	PBD	PBD1, PBD3	Putra-1× IRBB60 × MR219-PL-137
Three-way reciprocal cross	DPB	DPB7, DPB12, DPB13, DPB20	MR219-PL-137 × Putra-1 × IRBB60

Genotype	DTF (d)									PH (cm)											PL (cm)
Genotype	NS			RS			Pool		NS				RS			Pool					NS						RS				Pool
P	89.0 ± 0.7 a			NA			NA		103.2 ± 1.1 c				NA			NA					25.0 ± 0.5 ab					NA					NA
D	88.0 ± 0.7 ab			NA			NA		100.3 ± 1.5 c				NA			NA					25.9 ± 0.3 ab					NA					NA
B	87.8 ± 0.7 a-c			96.6 ± 0.6 a			92.2 ± 1.5 a		103.6 ± 1.6 c				97.5 ± 3.7 a			100.6 ± 2.2 b					25.1 ± 0.1 b					20.7 ± 0.9 c					22.9 ± 0.9 c
PB12	87.6 ± 0.4 a-c			NA			NA		104.3 ± 2.3 c				NA			NA					24.9 ± 0.1 b					NA					NA
PB15	86.8 ± 0.7 a-d			NA			NA		102.6 ± 1.8 c				NA			NA					25.9 ± 0.4 ab					NA					NA
PD14	85.8 ± 0.5 b-d			91.8 ± 0.4 d			88.8 ± 1.0 de		102.6 ± 2.3 c				96.7 ± 4.1 a			99.6 ± 2.4 b					25.3 ± 0.4 b					23.7 ± 0.5 ab					24.5 ± 0.4 ab
PD15	87.4 ± 0.5 a-d			92.8 ± 0.5 cd			90.1 ± 1.0 b-d		104.5 ± 2.8 c				97.3 ± 4.0 a			100.9 ± 2.6 b					24.7 ± 0.4 b					22.6 ± 0.5 ab					23.6 ± 0.5 bc
PBD1	87.6 ± 0.5 a-c			94.0 ± 0.6 bc			90.8 ± 1.1 a-c		104.7 ± 2.4 c				100.5 ± 3.8 a			102.6 ± 2.3 ab					24.9 ± 0.6 b					22.1 ± 0.5 bc					23.5 ± 0.6 bc
PBD3	88.0 ± 0.5 ab			94.0 ± 0.5 bc			91.0 ± 1.0 ab		99.9 ± 0.3 c				96.2 ± 4.4 a			98.1 ± 2.7 b					22.9 ± 0.6 c					23.3 ± 0.5 ab					24.5 ± 0.4 ab
PDB3	87.4 ± 0.5 a-d			92.8 ± 0.4 cd			90.1 ± 1.0 b-d		104.1 ± 3.0 c				100.6 ± 4.3 a			102.3 ± 2.6 ab					24.9 ± 0.4 b					24.1 ± 0.4 a					24.5 ± 0.3 ab
DPB7	85.2 ± 1.7 d			91.8 ± 0.4 d			88.5 ± 1.4 e		113.4 ± 2.1 a				102.1 ± 4.3 a			107.8 ± 2.9 a					25.0 ± 0.5 b					24.0 ± 1.0 a					24.5 ± 0.5 ab
DPB12	86.0 ± 1.0 b-d			92.8 ± 0.6 cd			89.4 ± 1.3 c-e		106.1 ± 1.1 bc				99.7 ± 3.5 a			102.9 ± 2.0 ab					25.0 ± 0.4 b					23.4 ± 0.3 ab					24.2 ± 0.4 ab
DPB13	86.2 ± 0.9 b-d			93.0 ± 0.7 d			89.6 ± 1.3 b-e		103.6 ± 2.4 c				98.4 ± 3.7 a			101.0 ± 2.2 b					25.2 ± 0.4 b					23.4 ± 0.3 ab					24.3 ± 0.4 ab
DPB20	85.6 ± 1.0 cd			95.0 ± 0.7 b			90.3 ± 1.7 bc		112.3 ± 3.0 ab				96.3 ± 4.9 a			104.3 ± 3.8 ab					26.6 ± 0.2 a					23.7 ± 0.6 ab					25.2 ± 0.6 a
Mean	87.03			93.46			90.08		104.67				98.54			102.01					25.09					23.09					24.02
CV (%)	2.03			1.30			1.79		4.74				9.35			7.28					4.07					5.87					5.05
LSD	2.25			1.56			1.43		6.29				11.82			6.61					1.30					1.74					1.08
h²_B(%)	66.27			88.09			75.05		28.63				20.22			76.52					31.13					74.83					4.76
Genotype	ET								TT									FFG
Genotype	NS	RS			Pool				NS		RS			Pool				NS				RS						Pool
P	10.2 ± 1.4 b-d	NA			NA				10.6 ± 1.6 b-e		NA			NA			172.8 ± 5.9 ab						NA					NA
D	13.6 ± 1.8 a	NA			NA				13.8 ± 1.7 a		NA			NA			168.4 ± 4.0 a-c						NA					NA
B	7.8 ± 1.3 de	9.0 ± 1.6 ab			8.4 ± 1.0 c				8.8 ± 1.0 de		9.6 ± 1.4 ab			9.2 ± 0.8 b/d			172.4 ± 9.3 ab						26.4 ± 2.9 c					99.4 d ± 24.8 e
PB12	9.4 ± 0.5 b-e	NA			NA				9.6 ± 0.5 c-e		NA			NA			177.6 ± 6.6 a						NA					NA
PB15	9.0 ± 1.1 c-e	NA			NA				9.2 ± 0.9 de		NA			NA			166.0 ± 5.8 a-d						NA					NA
PD14	10.6 ± 0.4 b-d	11.4 ± 1.2 a			11.0 ± 0.6 a				11.0 ± 0.5 a-d		11.4 ± 1.2 ab			11.2 ± 0.6 ab			178.4 ± 8.8 a						52.4 ± 2.3 ab					115.4 ± 21.4 a
PD15	9.6 ± 1.2 b-e	8.0 ± 1.4 b			8.8 ± 0.9 bc				11.0 ± 1.1 a-d		8.4 ± 1.2 b			9.7 ± 0.9 b-d			157.2 ± 1.2 cd						49.8 ± 5.2 ab					103.5 ± 18.1 cd
PBD1	11.0 ± 1.0 a-c	11.6 ± 0.5 a			11.3 ± 0.5 a				12.4 ± 1.5 a-c		11.6 ± 0.5 a			12.0 ± 0.8 a			170.6 ± 3.7 a-c						45.4 ± 2.8 b					108.0 ± 21.0 a-c
PBD3	12.0 ± 0.6 ab	10.6 ± 1.2 ab			11.3 ± 0.7 a				13.2 ± 0.9 ab		10.6 ± 1.2 ab			11.5 ± 0.8 ab			168.4 ± 2.7 a-c						59.4 ± 5.9 a					113.9 ± 18.4 ab
PDB3	11.2 ± 1.1 a-c	10.0 ± 1.3 ab			10.6 ± 0.8 ab				13.0 ± 1.1 ab		10.0 ± 1.3 ab			11.5 ± 1.0 ab			153.4 ± 2.0 d						48.2 ± 1.6 ab					100.8 ± 17.6 cd
DPB7	9.4 ± 0.5 b-e	11.8 ± 0.7 a			10.6 ± 0.6 ab				9.8 ± 0.5 c-e		11.8 ± 0.7 a			10.8 ± 0.5 a-c			161.4 ± 3.1 b-d						53.4 ± 3.8 ab					107.4 ± 18.2 a-d
DPB12	7.2 ± 0.6 e	9.2 ± 1.2 ab			8.2 ± 0.7 c				7.8 ± 0.5 e		9.4 ± 1.0 ab			8.6 ± 0.6 d			152.4 ± 1.7 d						52.2 ± 4.9 ab					102.3 ± 16.9 cd
DPB13	9.4 ± 0.5 c-e	10.2 ± 1.2 ab			9.8 ± 0.7 a-c				9.6 ± 0.6 c-e		11.4 ± 1.0 ab			10.5 ± 0.6 a-d			161.0 ± 3.2 b-d						43.8 ± 5.5 b					102.4 ± 19.8 cd
DPB20	9.0 ± 1.0 c-e	10.2 ± 1.1 ab			9.6 ± 0.7 a-c				9.2 ± 1.0 de		10.4 ± 1.0 ab			9.8 ± 0.7 b-d			165.8 ± 3.6 a-d						46.2 ± 4.0 b					106.0 ± 20.1 b-d
Mean	9.96	10.20			9.96				10.64		10.46			10.52			166.13						47.72					105.91
CV (%)	23.13	25.43			22.97				21.69		22.86			21.06			6.54						19.30					9.11
LSD	2.92	3.33			2.04				2.93		3.07			1.97			13.78						11.81					8.60
h²_B(%)	72.11	52.61			14.31				76.02		52.09			7.79			74.06						81.94					0
Genotype	HGW (g)									GLWR										GD (d)
Genotype	NS		RS			Pool				NS		RS			Pool					NS					RS					Pool
P	2.41 ± 0.03 de		NA			NA		4.97 ± 0.07bc				NA			NA				117.4 ± 0.2 ab					NA					NA
D	2.47 ± 0.08 c-e		NA			NA		4.98 ± 0.11 b				NA			NA				117.4 ± 0.2 ab					NA					NA
B	2.35 ± 0.07 e		2.43 ± 0.06 a			2.39 ± 0.04 c		5.61 ± 0.23 a				4.42 ± 0.04 b			5.01 ± 0.23 a				116.8 ± 0.2 b					133.2 ± 1.2 b					125.0 ± 2.8 a
PB12	2.55 ± 0.03 a-c		NA			NA		4.96 ± 0.02 bc				NA			NA				118.2 ± 0.4 a					NA					NA
PB15	2.49 c-e		NA			NA		4.81 b-d				NA			NA				117.6 ab					NA					NA
PD14	2.55 ± 0.23 a-c		2.34 ± 0.03 a			2.44 ± 0.04 bc		4.86 ± 0.04 b-d				4.49 ± 0.04 b			4.67 ± 0.07 b				117.4 ± 0.5 ab					128.4 ± 0.2 b					122.9 ± 1.9 b
PD15	2.52 ± 0.02 a-d		2.40 ± 0.05 a			2.46 ± 0.03 a-c		4.86 ± 0.04 b-d				4.49 ± 0.02 b			4.67 ± 0.06 b				117.6 ± 0.5 ab					127.6 ± 0.5 b					122.6 ± 1.7 b
PBD1	2.63 ± 0.04 a		2.42 ± 0.05 a			2.52 ± 0.05 a		4.59 ± 0.04 b-d				4.51 ± 0.01 b			4.55 ± 0.03 bc				117.6 ± 0.4 ab					127.8 ± 0.4b					122.7 ± 1.7 b
PBD3	2.52 ± 0.04 a-d		2.38 ± 0.05 a			2.45 ± 0.04 a-c		4.63 ± 0.08 b-d				4.47 ± 0.04 b			4.55 ± 0.05 b				117.6 ± 0.5 ab					129.0 ± 0.5 b					123.3 ± 1.9 b
PDB3	2.47 ± 0.04 c-e		2.41 ± 0.03 a			2.44 ± 0.02 bc		4.53 ± 0.02 d				4.50 ± 0.02 b			4.51 ± 0.02 bc				117.0 ± 0.6 ab					127.8 ± 0.6 b					122.4 ± 1.8 b
DPB7	2.52 ± 0.06 a-d		2.32 ± 0.02 a			2.42 ± 0.05 bc		4.51 ± 0.04 cd				4.57 ± 0.03 ab			4.54 ± 0.02 bc				117.0 ± 0.7 ab					127.4 ± 0.5 ab					122.2 ± 1.8 b
DPB12	2.52 ± 0.03 a-d		2.35 ± 0.03 a			2.43 ± 0.04 bc		4.57 ± 0.04 cd				4.75 ± 0.20 a			4.66 ± 0.10 b				117.6 ± 0.9 ab					128.2 ± 0.4 a					122.9 ± 1.8 b
DPB13	2.60 ± 0.03 ab		2.39 ± 0.03 a			2.49 ± 0.04 ab		4.51 ± 0.04 d				4.57 ± 0.03 ab			4.54 ± 0.02 bc				117.0 ± 0.7 ab					128.2 ± 0.6 ab					122.6 ± 1.9 b
DPB20	2.56 ± 0.03 a-c		2.43 ± 0.02 a			2.50 ± 0.03 ab		4.08 ± 0.43 e				4.53 ± 0.05 b			4.31 ± 0.22 c				117.8 ± 0.4 ab					127.6 ± 0.7 b					122.7 ± 1.7 b
Mean	2.51		2.39			2.45		4.75				4.53			4.60				117.43					128.52					122.93
CV	3.83		3.75			3.67		6.63				3.50			5.99				0.88					1.09					1.03
LSD	0.12		0.11			0.08		0.40				0.20			0.25				1.31					1.80					1.12
h²_B(%)	66.67		50.00			0		85.92				57.14			0				39.77					88.13					0

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Metrics

Genotype	Marker	Gene/QTL	Chr.	Annealing temperature (ºC)	Repeat motif	Expected base pair size (bp)	Reference
IRBB60	RM224	Xa4	11	55	(AAG)8(AG)13	157	He et al, 2006; Tanweer et al, 2015
	RM122	xa5	5	-	(GA)7A(GA)2A(GA)11	227	Wu and Tanksley,1993; Khan et al, 2015
	RM153	xa5	5	55	(GAA)9	201	Ashkani et al, 2011
	RM13	xa5	5	55	(GA)6-(GA)16	141	Khan et al, 2015
	RG136 Xa13Prom	xa13	8	-	-	246	Zhang et al, 1996
	RG136 Xa13Prom	xa13	8	-	-	246	Chukwu et al, 2019; Akos et al, 2019b, c
	RM21	Xa-21	11	55	(GA)18	157	Chen et al, 1997; Pradhan et al, 2015
	pTA248	Xa-21	11	-	-	157	Ronald et al, 1992
Putra-1	RM8225	Piz	6	55	A11N(AAG)14	221	Miah et al, 2016; Akos et al, 2019b, c
Putra-1	RM6836	Piz, Pi2, Pi9	6	55	(TCT)14	240	Ashkani et al, 2011; Akos et al, 2019b, c
MR219-PL-137	RM236	qDTY_2.2	2	55	(CT)18	174	Swamy et al, 2013; Shamsudin et al, 2016
	RM276	qDTY_2.2,3.1	6	55	(AG)8A3(GA)33	149	Shamsudin et al, 2016
	RM511	qDTY_12.1	12	55	(GAC)7	130	Mishra et al, 2013; Bernier et al, 2007; Shamsudin et al, 2016
	RM520	qDTY_3.2	3	55	(AG)10	247	Shamsudin et al, 2016
	RM1261	qDTY_12.1	12	50	(AG)16	167	Mishra et al, 2013; Bernier et al, 2007

Trait	Treatment	DTF	PH	PL	ET	TT	FFG	HGW	GLWR
PH	NS	-0.263*
	RS	-0.113
	Pool	-0.444**
PL	NS	-0.202	0.306**
	RS	-0.349*	0.040
	Pool	-0.611**	0.390**
ET	NS	0.006	-0.073	-0.118
	RS	-0.128	0.026	0.065
	Pool	0.065	-0.053	-0.120
TT	NS	0.002	-0.120	-0.245*	0.925**
	RS	-0.111	0.030	0.053	0.982**
	Pool	-0.024	-0.041	-0.119	0.927**
FFG	NS	0.004	-0.136	0.069	0.082	-0.008
	RS	-0.358*	-0.064	0.248	0.153	0.111
	Pool	-0.873**	0.391**	0.568**	-0.068	0.035
HGW	NS	-0.277*	0.071	0.069	0.015	-0.037	0.137
	RS	0.269	-0.004s	-0.187	-0.134	-0.133	-0.118
	Pool	-0.510**	0.246*	0.300**	-0.010	0.013	0.563**
GLWR	NS	0.342**	-0.262*	-0.118	0.011	0.008	0.092	-0.299
	RS	-0.217	0.086	0.172	0.073	0.071	0.203	-0.0004
	Pool	-0.094	-0.027	0.046	-0.032	0.017	0.204*	-0.126
GD	NS	-0.014	0.138	0.054	0.112	0.040	0.233	0.176	-0.126
	RS	0.590**	-0.111	-0.532**	-0.083	-0.061	-0.433*	0.138	-0.226
	Pool	0.886**	-0.412**	-0.606**	0.097	-0.022	-0.951**	-0.511**	-0.227*

Source	DF				DTF		PH		PL				ET
Source	NS		RS		NS	RS	NS	RS	NS		RS		NS	RS
Replication	4		4		3.31ns	0.93ns	50.4ns	74.74ns	0.28ns		0.62ns		2.13ns	8.65ns
Genotype	13		9		6.15*	11.02**	73.9**	21.53ns	3.39*		5.47**		13.73ns	7.47ns
Error	52		36		3.13	1.49	24.6	84.94	1.04		1.84		5.31	6.73
Source		TT			FFG		100-GW		GLW			YM
Source		NS		RS	NS	RS	NS	RS	NS	RS		NS		RS
Replication		5.32ns		7.13ns	239.19ns	86.57ns	0.02ns	0.01ns	0.07ns	0.02ns		1.68ns		0.97ns
Genotype		16.90**		6.22ns	336.66*	384.90**	0.02**	0.01ns	0.61**	0.04ns		0.70ns		14.63**
Error		5.33		5.72	117.92	84.83	0.01	0.01	0.10	0.03		0.01		1.97

Source of Var.	DF	DTF (cm)	PH (cm)	PL (cm)	ET (no)	TT (no)	FFG (no)	100GW (g)	GLW	YM (days)
Replications	4	1.17ns	101.74ns	0.69ns	7.59ns	7.97ns	215.44ns	0.016ns	0.05ns	1.42ns
RS-TRT	1	1142.44**	1205.69**	86.49**	5.76ns	0.36ns	338607.61**	0.47**	0.51**	3124.81**
G	9	11.93**	72.66ns	5.27**	13.92**	13.57**	289.05**	0.02ns	0.32**	6.18**
RS-TRT×G	9	4.48ns	38.00ns	4.26**	4.98ns	9.74*	458.57**	0.02*	0.49**	9.08**
Error	76	2.59	55.15	1.47	5.23	4.91	93.16	0.01	0.08	1.6