Fig. 1. Variability in transpiration among rice genotypes with differing epicuticular wax (Ewax) content at varying night temperatures. A, Cumulative water transpired (CWT) over the entire night (18:00 to 6:00, CWTnight), CWT during the early night (18:00 to 0:00, CWTearly night), CWT during the late night (0:00 to 6:00, CWTlate night), and CWT during predawn hours (4:00 to 6:00, CWTpredawn) were measured in rice seedlings from 20 to 100 d after sowing. Mean transpiration rate (MTR) was calculated during the entire night (MTRnight), early night (MTRearly night), late night (MTRlate night), and predawn hours (MTRpredawn). Data are mean ± SD (n = 3). Different lowercase letters above bars indicate significant differences at P < 0.05 by one-way analysis of variance. B, CWTnight, percentage increase in CWT relative to 20 ºC, MTRnight, and percentage increase in MTR relative to 20 ºC were measured. IRG349 and IRG066 are high-Ewax genotypes, IRG355 and IRG263 are moderate-Ewax genotypes, and IRG277 and IRG335 are low-Ewax genotypes.