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Rice Science ›› 2016, Vol. 23 ›› Issue (4): 211-218.DOI: 10.1016/j.rsci.2016.06.004

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  • 收稿日期:2015-06-08 接受日期:2015-12-14 出版日期:2016-07-28 发布日期:2016-04-11

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链接本文: http://www.ricesci.org/CN/10.1016/j.rsci.2016.06.004

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Table 1 List of 31 duplicate and similar named rice germplasms of Bangladesh.
Serial No. Accession name Accession No. a Origin
KS1 Kartik Sail 3243 Sylhet
KS2 Kartik Sail 776 Chittagong
KS3 Katih Shail 438 Rajshahi
KS4 Kartik Sail 539 Rangpur
KS5 Kartik Sail 77 Dhaka
KS6 Kati Shail 170 Tangail
KS7 Kartik Sail 3662 Sherpur
KS8 Kati Shail 3631 Rajshahi
KS9 Kartika 4053 Sylhet
KS10 Kartik Sail 4881 Tangail
KS11 Kartik Sail 76 Dhaka
KS12 Kati Shail 437 Rajshahi
KS13 Kartik Sail 78 Dhaka
KS14 Kartik Sail 1882 Kishorganj
KS15 Kartik sail (2) 689 Comilla
KS16 Kartik sail (2) 846 Sylhet
KS17 Kartik Sail 664 Comilla
KS18 Kartik Sail 1887 Kishorganj
KS19 Kartik Sail 844 Sylhet
KS20 Katih Shail 994 Khulna
KS21 Kartik Sail 845 Sylhet
DB1 Dhali Boro 2250 Sylhet
DB2 Dhali Boro 2247 Sylhet
DB3 Dhali Boro 2249 Sylhet
DB4 Dholi Boro 180 Tangail
DB5 Dhali Boro 2245 Sylhet
DB6 Dholi Boro 4396 Sylhet
DB7 Dhali Boro 2246 Sylhet
DB8 Dhali Boro 2244 Sylhet
DB9 Dhali Boro 2248 Sylhet
DB10 Dhali Boro 2243 Sylhet

Table 1 List of 31 duplicate and similar named rice germplasms of Bangladesh.

Serial No. Accession name Accession No. a Origin
KS1 Kartik Sail 3243 Sylhet
KS2 Kartik Sail 776 Chittagong
KS3 Katih Shail 438 Rajshahi
KS4 Kartik Sail 539 Rangpur
KS5 Kartik Sail 77 Dhaka
KS6 Kati Shail 170 Tangail
KS7 Kartik Sail 3662 Sherpur
KS8 Kati Shail 3631 Rajshahi
KS9 Kartika 4053 Sylhet
KS10 Kartik Sail 4881 Tangail
KS11 Kartik Sail 76 Dhaka
KS12 Kati Shail 437 Rajshahi
KS13 Kartik Sail 78 Dhaka
KS14 Kartik Sail 1882 Kishorganj
KS15 Kartik sail (2) 689 Comilla
KS16 Kartik sail (2) 846 Sylhet
KS17 Kartik Sail 664 Comilla
KS18 Kartik Sail 1887 Kishorganj
KS19 Kartik Sail 844 Sylhet
KS20 Katih Shail 994 Khulna
KS21 Kartik Sail 845 Sylhet
DB1 Dhali Boro 2250 Sylhet
DB2 Dhali Boro 2247 Sylhet
DB3 Dhali Boro 2249 Sylhet
DB4 Dholi Boro 180 Tangail
DB5 Dhali Boro 2245 Sylhet
DB6 Dholi Boro 4396 Sylhet
DB7 Dhali Boro 2246 Sylhet
DB8 Dhali Boro 2244 Sylhet
DB9 Dhali Boro 2248 Sylhet
DB10 Dhali Boro 2243 Sylhet
Fig. 1. Scatter diagram of 31 duplicate and similar named rice germplasms and the check BR23.

Fig. 1. Scatter diagram of 31 duplicate and similar named rice germplasms and the check BR23.

Table 2 Distribution of 31 duplicate and similar named rice germplasms into 4 clusters for 18 morpho-physicochemical characters.
Cluster No. of genotypes Serial No.
I 11 KS1, KS2, KS5, KS7, KS9, KS11, KS14, KS17, KS18, KS19, KS21
II 3 KS6, KS8, KS13
III 7 KS3, KS4, KS10, KS12, KS15, KS16, KS20
IV 10 DB1, DB2, DB3, DB4, DB5, DB6, DB7, DB8, DB9, DB10

Table 2 Distribution of 31 duplicate and similar named rice germplasms into 4 clusters for 18 morpho-physicochemical characters.

Cluster No. of genotypes Serial No.
I 11 KS1, KS2, KS5, KS7, KS9, KS11, KS14, KS17, KS18, KS19, KS21
II 3 KS6, KS8, KS13
III 7 KS3, KS4, KS10, KS12, KS15, KS16, KS20
IV 10 DB1, DB2, DB3, DB4, DB5, DB6, DB7, DB8, DB9, DB10
Table 3 Average intra- (bold) and inter-cluster distances (D2) for 18 morpho-physicochemical characters of 31 duplicate and similar named rice germplasms.
Cluster I II III IV
I 0.93
II 6.91 0.69
III 5.07 10.21 0.74
IV 24.61 22.17 22.06 0.62

Table 3 Average intra- (bold) and inter-cluster distances (D2) for 18 morpho-physicochemical characters of 31 duplicate and similar named rice germplasms.

Cluster I II III IV
I 0.93
II 6.91 0.69
III 5.07 10.21 0.74
IV 24.61 22.17 22.06 0.62
Table 4 Means and latent vectors for 18 morpho-physicochemical characters of 31 duplicate and similar named rice germplasms.
Character Cluster I Cluster II Cluster III Cluster IV Vector I Vector II Combined ranking a
Seedling height (cm) 66 69.9 56.7 34.8 0.1093 -0.0729 9
Plant height (cm) 123.9 125.6 111.1 106.1 -0.1082 0.0332 12
Culm diameter (mm) 5.27 5.16 4.77 4.28 1.7199 -1.788 11
Panicle exsertion (mm) 0.64 0.53 0.44 6.13 -1.6266 -0.2472 18
Effective tillering number per hill 11.8 11 12.1 10.3 0.2065 0.2738 3
Days to maturity (d) 143 141 142 160 -0.2783 -0.1692 14
Primary branch number per panicle 10.6 9 9.1 7.1 0.847 0.1551 2
Secondary branch number per panicle 33.6 21.7 24.9 16.9 0.1115 0.24 6
Grain yield per panicle (g) 3.2 2.7 2.3 1.9 0.2634 -1.1171 17
Grain length (mm) 8.55 8.44 8.17 7.62 0.2474 -0.3449 13
Awn length (mm) 0.6 21.5 0 30.5 -0.0198 0.1587 7
1000-grain weight (g) 23.19 27.18 20.82 20.69 -0.0652 0.5106 4
Grain yield per hill (g) 22.42 21.28 22 17.28 -0.3271 -0.1641 15
Biological yield (g) 40.9 37 33.1 27.7 0.1759 0.2617 5
Milling rate (%) 68.2 65.3 69.9 68.7 0.0361 -0.633 16
Cooking time (min) 17 20 16.5 19.1 -0.4281 0.3856 10
Amylose content (%) 25.3 25.27 25.76 21.17 0.2999 -0.2623 8
Protein content (%) 7.65 9.03 7.92 9.12 0.8121 0.6391 1

Table 4 Means and latent vectors for 18 morpho-physicochemical characters of 31 duplicate and similar named rice germplasms.

Character Cluster I Cluster II Cluster III Cluster IV Vector I Vector II Combined ranking a
Seedling height (cm) 66 69.9 56.7 34.8 0.1093 -0.0729 9
Plant height (cm) 123.9 125.6 111.1 106.1 -0.1082 0.0332 12
Culm diameter (mm) 5.27 5.16 4.77 4.28 1.7199 -1.788 11
Panicle exsertion (mm) 0.64 0.53 0.44 6.13 -1.6266 -0.2472 18
Effective tillering number per hill 11.8 11 12.1 10.3 0.2065 0.2738 3
Days to maturity (d) 143 141 142 160 -0.2783 -0.1692 14
Primary branch number per panicle 10.6 9 9.1 7.1 0.847 0.1551 2
Secondary branch number per panicle 33.6 21.7 24.9 16.9 0.1115 0.24 6
Grain yield per panicle (g) 3.2 2.7 2.3 1.9 0.2634 -1.1171 17
Grain length (mm) 8.55 8.44 8.17 7.62 0.2474 -0.3449 13
Awn length (mm) 0.6 21.5 0 30.5 -0.0198 0.1587 7
1000-grain weight (g) 23.19 27.18 20.82 20.69 -0.0652 0.5106 4
Grain yield per hill (g) 22.42 21.28 22 17.28 -0.3271 -0.1641 15
Biological yield (g) 40.9 37 33.1 27.7 0.1759 0.2617 5
Milling rate (%) 68.2 65.3 69.9 68.7 0.0361 -0.633 16
Cooking time (min) 17 20 16.5 19.1 -0.4281 0.3856 10
Amylose content (%) 25.3 25.27 25.76 21.17 0.2999 -0.2623 8
Protein content (%) 7.65 9.03 7.92 9.12 0.8121 0.6391 1
Table 5 Allele variation, gene diversity and polymorphism information content of 45 simple sequence repeat markers across 31 duplicate and similar named rice germplasms.
Chromosome Marker Position No. of alleles Size Major allele No. of No. of Gene Polymorphism information content
(cM) (bp) Size (bp) Frequency (%) rare alleles null alleles diversity
1 RM283 31.4 5 141-150 150 44 4 1 0.62 0.55
RM259 54.2 6 166-187 170 39 4 0 0.76 0.73
RM237 115.2 6 116-133 130 33 3 1 0.78 0.75
RM302 147.8 9 107-214 178 31 7 2 0.82 0.8
RM154 4.8 8 157-183 163/167 22 6 0 0.84 0.82
2 RM279 17.3 8 135-173 166 33 6 3 0.82 0.8
RM324 66 9 132-166 166 28 7 0 0.82 0.8
RM250 170.1 5 149-155 152 61 4 0 0.59 0.56
RM60 0 10 147-182 162 25 8 1 0.86 0.85
3 RM218 67.8 10 126-152 136 22 8 3 0.86 0.84
RM55 168.2 10 215-240 229 17 9 3 0.89 0.88
RM227 214.7 4 97-105 97 47 1 1 0.66 0.6
RM307 0 6 123-177 131 69 5 3 0.5 0.48
4 RM273 94.4 7 200-217 202 39 4 0 0.77 0.74
RM241 106.2 7 100-130 126 44 5 0 0.72 0.69
RM127 150.1 4 212-224 218 56 1 0 0.61 0.56
RM413 26.7 9 68-105 68 22 7 2 0.86 0.85
5 RM267 28.6 8 150-185 166 36 6 0 0.75 0.71
RM161 96.9 6 166-182 171 36 3 0 0.77 0.73
RM133 0 4 225-232 227 69 2 0 0.48 0.44
6 RM584 26.2 10 160-214 163 33 8 0 0.83 0.81
RM541 75.5 11 142-194 160 25 9 0 0.84 0.83
RM162 108.3 6 223-233 223 33 3 0 0.77 0.74
RM125 24.8 4 130-145 141 61 1 0 0.56 0.51
7 RM214 34.7 8 110-137 117 33 5 0 0.78 0.75
RM18 90.4 8 160-180 169 22 5 1 0.85 0.84
RM337 1.1 6 162-202 168 42 3 0 0.71 0.67
8 RM223 80.5 8 138-163 155 31 5 0 0.82 0.8
RM256 101.5 8 103-138 108 36 5 0 0.78 0.76
RM433 116 13 207-248 237 28 12 1 0.87 0.86
RM296 0 8 121-139 125 39 6 0 0.72 0.68
9 RM242 73.3 8 190-214 203 28 6 0 0.83 0.81
RM215 99.4 7 159-176 163 31 6 1 0.83 0.81
RM311 25.2 10 170-201 176/182 19 7 0 0.86 0.84
10 RM271 59.4 8 81-98 93 28 6 1 0.82 0.8
RM258 70.8 13 139-167 146 22 11 1 0.88 0.87
RM171 92.8 6 314-340 314 36 4 0 0.75 0.72
RM21 85.7 14 129-173 166 22 12 0 0.89 0.88
11 RM229 77.8 8 105-129 112 25 5 0 0.82 0.8
RM206 102.9 13 116-171 157 17 12 0 0.9 0.89
RM224 120.1 9 123-155 147 36 7 0 0.8 0.78
RM286 0 6 96-121 96 31 4 0 0.79 0.75
12 RM19 20.9 8 223-239 228 28 5 1 0.83 0.81
RM247 32.3 6 126-144 136 31 3 0 0.78 0.75
RM277 57.2 3 116-123 116/119 47 1 0 0.55 0.45
Minimum 3 68 17 1 0 0.48 0.44
Average 7.8 161.2 35 5.8 0.6 0.77 0.74
Maximum 14 314 69 12 3 0.9 0.89

Table 5 Allele variation, gene diversity and polymorphism information content of 45 simple sequence repeat markers across 31 duplicate and similar named rice germplasms.

Chromosome Marker Position No. of alleles Size Major allele No. of No. of Gene Polymorphism information content
(cM) (bp) Size (bp) Frequency (%) rare alleles null alleles diversity
1 RM283 31.4 5 141-150 150 44 4 1 0.62 0.55
RM259 54.2 6 166-187 170 39 4 0 0.76 0.73
RM237 115.2 6 116-133 130 33 3 1 0.78 0.75
RM302 147.8 9 107-214 178 31 7 2 0.82 0.8
RM154 4.8 8 157-183 163/167 22 6 0 0.84 0.82
2 RM279 17.3 8 135-173 166 33 6 3 0.82 0.8
RM324 66 9 132-166 166 28 7 0 0.82 0.8
RM250 170.1 5 149-155 152 61 4 0 0.59 0.56
RM60 0 10 147-182 162 25 8 1 0.86 0.85
3 RM218 67.8 10 126-152 136 22 8 3 0.86 0.84
RM55 168.2 10 215-240 229 17 9 3 0.89 0.88
RM227 214.7 4 97-105 97 47 1 1 0.66 0.6
RM307 0 6 123-177 131 69 5 3 0.5 0.48
4 RM273 94.4 7 200-217 202 39 4 0 0.77 0.74
RM241 106.2 7 100-130 126 44 5 0 0.72 0.69
RM127 150.1 4 212-224 218 56 1 0 0.61 0.56
RM413 26.7 9 68-105 68 22 7 2 0.86 0.85
5 RM267 28.6 8 150-185 166 36 6 0 0.75 0.71
RM161 96.9 6 166-182 171 36 3 0 0.77 0.73
RM133 0 4 225-232 227 69 2 0 0.48 0.44
6 RM584 26.2 10 160-214 163 33 8 0 0.83 0.81
RM541 75.5 11 142-194 160 25 9 0 0.84 0.83
RM162 108.3 6 223-233 223 33 3 0 0.77 0.74
RM125 24.8 4 130-145 141 61 1 0 0.56 0.51
7 RM214 34.7 8 110-137 117 33 5 0 0.78 0.75
RM18 90.4 8 160-180 169 22 5 1 0.85 0.84
RM337 1.1 6 162-202 168 42 3 0 0.71 0.67
8 RM223 80.5 8 138-163 155 31 5 0 0.82 0.8
RM256 101.5 8 103-138 108 36 5 0 0.78 0.76
RM433 116 13 207-248 237 28 12 1 0.87 0.86
RM296 0 8 121-139 125 39 6 0 0.72 0.68
9 RM242 73.3 8 190-214 203 28 6 0 0.83 0.81
RM215 99.4 7 159-176 163 31 6 1 0.83 0.81
RM311 25.2 10 170-201 176/182 19 7 0 0.86 0.84
10 RM271 59.4 8 81-98 93 28 6 1 0.82 0.8
RM258 70.8 13 139-167 146 22 11 1 0.88 0.87
RM171 92.8 6 314-340 314 36 4 0 0.75 0.72
RM21 85.7 14 129-173 166 22 12 0 0.89 0.88
11 RM229 77.8 8 105-129 112 25 5 0 0.82 0.8
RM206 102.9 13 116-171 157 17 12 0 0.9 0.89
RM224 120.1 9 123-155 147 36 7 0 0.8 0.78
RM286 0 6 96-121 96 31 4 0 0.79 0.75
12 RM19 20.9 8 223-239 228 28 5 1 0.83 0.81
RM247 32.3 6 126-144 136 31 3 0 0.78 0.75
RM277 57.2 3 116-123 116/119 47 1 0 0.55 0.45
Minimum 3 68 17 1 0 0.48 0.44
Average 7.8 161.2 35 5.8 0.6 0.77 0.74
Maximum 14 314 69 12 3 0.9 0.89
Fig. 2. DNA profile of 36 rice genotypes using RM224 marker.1, KS1; 2, KS2; 3, KS3; 4, KS4; 5, KS5; 6, KS6; 7, KS7; 8, KS8; 9, KS9; 10, KS10; 11, KS11; 12, KS12; 13, KS13; 14, KS14; 15, KS15; 16, KS16; 17, KS17; 18, KS18; 19, KS19; 20, KS20; 21, KS21; 22, BR4; 23, BR23; 24, DB1; 25, DB2; 26, DB3; 27, DB4; 28, DB5; 29, DB6; 30, DB7; 31, DB8; 32, DB9; 33, DB10; 34, BRRI dhan 28; 35, BRRI dhan 29; 36, BR14.

Fig. 2. DNA profile of 36 rice genotypes using RM224 marker.1, KS1; 2, KS2; 3, KS3; 4, KS4; 5, KS5; 6, KS6; 7, KS7; 8, KS8; 9, KS9; 10, KS10; 11, KS11; 12, KS12; 13, KS13; 14, KS14; 15, KS15; 16, KS16; 17, KS17; 18, KS18; 19, KS19; 20, KS20; 21, KS21; 22, BR4; 23, BR23; 24, DB1; 25, DB2; 26, DB3; 27, DB4; 28, DB5; 29, DB6; 30, DB7; 31, DB8; 32, DB9; 33, DB10; 34, BRRI dhan 28; 35, BRRI dhan 29; 36, BR14.

Fig. 3. Dendrogram of 31 duplicate and similar named rice germplasms derived from unweighted pair-group method with arithmetic means cluster analysis based on Nei’s genetic distance across 45 simple sequence repeat markers.BR28, BRRI dhan 28; BR29, BRRI dhan 29.

Fig. 3. Dendrogram of 31 duplicate and similar named rice germplasms derived from unweighted pair-group method with arithmetic means cluster analysis based on Nei’s genetic distance across 45 simple sequence repeat markers.BR28, BRRI dhan 28; BR29, BRRI dhan 29.

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