Influence of Temperature on Symbiotic Bacterium Composition in Successive Generations of Egg Parasitoid, Anagrus nilaparvatae

doi:10.1016/j.rsci.2016.06.003

摘要/Abstract

. [J]. Rice Science, 2016, 23(4): 203-209.

Fig. 1. Denatured gradient gel electrophoresis (DGGE) profile of 16S rDNA V3 fragments of bacteria associated with generations of A. nilaparvatae cultured under different temperatures. M, Marker; T1, T2, T3, T4 and T5 corresponding to 22 °C, 25 °C, 28 °C, 31 °C and 34 °C, respectively; F0 to F4 corresponding to 0 to 4th generations. All marked bands were analyzed by sequence analysis.

Fig. 2. Principal component analysis of denatured gradient gel electrophoresis (DGGE) profiles. Plots indicate the bacterial community structure of samples; Legends showed the different temperatures and generations.T1, T2, T3, T4 and T5 corresponding to 22 °C, 25 °C, 28 °C, 31 °C and 34 °C, respectively; F0 to F4 corresponding to 0 to 4th generations.

Fig. 3. Phylogenetic tree of bacteria associated with generations of A. nilaparvatae cultured under different temperatures. Distances were calculated using the maximum-likelihood method, and the tree was constructed using neighbor-jonining method.

Fig. 4. Denatured gradient gel electrophoresis (DGGE) profile of wsp gene fragments of Wolbachia in generations of A. nilaparvatae cultured under different temperatures. T1, T2, T3, T4 and T5 corresponding to 22 °C, 25 °C, 28 °C, 31 °C and 34 °C, respectively; F0 to F4 corresponding to 0 to 4th generations. All marked bands were analyzed by sequence analysis.

Fig. 5. Phylogenetic tree of wsp gene of Wolbachia in generations of A. nilaparvatae cultured under different temperatures.Distances were calculated using the maximum-likelihood method, and the tree was constructed using neighbor-jonining method.

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