Fig. 1. Responses of Rasi and Tellahamsa seedlings to salinity stress. A, Phenotypes of Rasi and Tellahamsa seedlings at 12 h of NaCl stress; B, Phenotypes of Rasi and Tellahamsa seedlings at 24 h of NaCl stress; C, Percent reductions in root length (RL) and shoot length (SL) of Rasi and Tellahamsa over its control at 12 h; D, Percent reductions in root length (RL) and shoot length (SL) of Rasi and Tellahamsa over its control at 24 h. 100-250 mmol/L NaCl represents 100 mmol/L NaCl for 12 h followed by 250 mmol/L NaCl for 12 h. Significant differences at the 0.05 level are indicated by lowercase letters.

Fig. 2. Expression analyses of OsLEA14 in the seedlings of Rasi and Tellahamsa. Lanes 1, 2 and 3 correspond to expression in Tellahamsa, whereas lanes 4, 5 and 6 correspond to expression in Rasi. At 12 h, lanes 1 and 4 represent induction stress with 100 mmol/L NaCl; At 24 h, lanes 1 and 4 represent 100 mmol/L NaCl for 12 h followed by 250 mmol/L NaCl for 12 h; Lanes 2 and 5 are in lethal stress with 250 mmol/L NaCl, and lanes 3 and 6 are in control (distilled water) at 12 h and 24 h, respectively.

Fig. 3. Expression analyses of OsWRKY72a by semi-quantitative reverse transcriptase-PCR. Lanes 1, 2 and 3 correspond to expression in Tellahamsa, whereas lanes 4, 5 and 6 correspond to expression in Rasi. At 12 h, lanes 1 and 4 represent induction stress with 100 mmol/L NaCl; At 24 h, lanes 1 and 4 represent 100 mmol/L NaCl for 12 h followed by 250 mmol/L NaCl for 12 h; Lanes 2 and 5 are in lethal stress with 250 mmol/L NaCl, and lanes 3 and 6 are in control (distilled water) at 12 h and 24 h, respectively.

Fig. 4. Identification of OsWRKY72b in indica rice genotype. Alignments of OsWRKY72a and OsWRKY72b amino acid sequence. The region within red colored arrow indicates the WRKY domain, and additional sequence present in OsWRKY72b is represented by colored amino acids.

Fig. 5. Expression of OsWRKY72b in seedlings of Rasi and Tellahamsa under salinity stress. Lanes 1, 2 and 3 correspond to expression in Tellahamsa, whereas lanes 4, 5 and 6 correspond to expression in Rasi. At 12 h, lanes 1 and 4 represent induction stress with 100 mmol/L NaCl; At 24 h, lanes 1 and 4 represent 100 mmol/L NaCl for 12 h followed by 250 mmol/L NaCl for 12 h; Lanes 2 and 5 are in lethal stress with 250 mmol/L NaCl, and lanes 3 and 6 are in control (distilled water) at 12 h and 24 h, respectively.

Fig. 6. In silico analysis of OsWKY72a and OsWKY72b. A and C, Predictor of natural disordered regions (PONDR) profiles for disorder; B and D, Phosphorylation site prediction using NetPhos 2 serv. The black rectangle on the threshold line indicates the region interacts with its binding partners.