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Rice Science ›› 2017, Vol. 24 ›› Issue (1): 41-47.DOI: 10.1016/j.rsci.2016.07.004

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  • 收稿日期:2016-05-04 接受日期:2016-07-03 出版日期:2017-01-10 发布日期:2016-11-01

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链接本文: http://www.ricesci.org/CN/10.1016/j.rsci.2016.07.004

               http://www.ricesci.org/CN/Y2017/V24/I1/41

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Table 1 Details of single nucleotide polymorphisms and gene based sequence-tagged site markers tightly linked to the major rice blast resistant genes.
Gene Chr Marker Forward (5′-3′) Reverse (5′-3′) AT (°C) ES (bp) Reference
Pi-d2 6 ttggctatcataggcgtcc atttgaaggcgtttgcgtaga 55 1 057 Chen et al (2006)
Pi-36 8 caatgtgtgacttgtgcggact tcttccatctcggatttcgtgt 55 1 036 Jin et al (2011)
Pi-37 1 tcttgagggtcccagtgtac cgaacagtggctggtatctc 55 1 149 Sun (2012)
Pi5 9 tcctcctcttcggacacctc cggacgagcgatagtgatcc 55 594 Sun (2012)
Pi-z 6 Z56592 ggacccgcgttttccacgtgtaa aggaatctattgctaagcatgac 60 292 Hayashi et al (2004)
Piz-t 6 Zt56591 ttgctgagccattgttaaaca atctcttcatatatatgaaggccac 60 257 Hayashi et al (2006)
Pik-p 11 K3957 atagttgaatgtatggaatggaat ctgcgccaagcaataaagtc 60 148 Hayashi et al (2006)
Pik-h 11 Candi gene marker catgagttccatttactattcctc acattggtagtagtgcaatgtca 55 1 500 Sharma et al (2005)
Pi-b 2 Pb28 gactcggtcgaccaattcgcc atcaggccaggccagatttg 60 388 Hayashi et al (2006)
Pi-9 6 195R-1 atggtcctttatctttattg ttgctccatctcctctgtt 56 2 000 Qu et al (2006)
Pi-ta/Pi-ta2 12 YL155/YL87 agcaggttataagctaggcc ctaccaacaagttcatcaaa 55 1 042 Jia et al (2002, 2004)
Chr, Chromosome; AT, Annealling temperature; ES, Expected size.

Table 1 Details of single nucleotide polymorphisms and gene based sequence-tagged site markers tightly linked to the major rice blast resistant genes.

Gene Chr Marker Forward (5′-3′) Reverse (5′-3′) AT (°C) ES (bp) Reference
Pi-d2 6 ttggctatcataggcgtcc atttgaaggcgtttgcgtaga 55 1 057 Chen et al (2006)
Pi-36 8 caatgtgtgacttgtgcggact tcttccatctcggatttcgtgt 55 1 036 Jin et al (2011)
Pi-37 1 tcttgagggtcccagtgtac cgaacagtggctggtatctc 55 1 149 Sun (2012)
Pi5 9 tcctcctcttcggacacctc cggacgagcgatagtgatcc 55 594 Sun (2012)
Pi-z 6 Z56592 ggacccgcgttttccacgtgtaa aggaatctattgctaagcatgac 60 292 Hayashi et al (2004)
Piz-t 6 Zt56591 ttgctgagccattgttaaaca atctcttcatatatatgaaggccac 60 257 Hayashi et al (2006)
Pik-p 11 K3957 atagttgaatgtatggaatggaat ctgcgccaagcaataaagtc 60 148 Hayashi et al (2006)
Pik-h 11 Candi gene marker catgagttccatttactattcctc acattggtagtagtgcaatgtca 55 1 500 Sharma et al (2005)
Pi-b 2 Pb28 gactcggtcgaccaattcgcc atcaggccaggccagatttg 60 388 Hayashi et al (2006)
Pi-9 6 195R-1 atggtcctttatctttattg ttgctccatctcctctgtt 56 2 000 Qu et al (2006)
Pi-ta/Pi-ta2 12 YL155/YL87 agcaggttataagctaggcc ctaccaacaagttcatcaaa 55 1 042 Jia et al (2002, 2004)
Chr, Chromosome; AT, Annealling temperature; ES, Expected size.
Table 2 Single nucleotide polymorphisms and sequence-tagged site markers associated with eleven blast resistance genes in 32 rice germplasms.
Accession Pi-d2 Pi-z Piz-t Pi-9 Pi-36 Pi-37 Pi5 Pi-b Pi-ta2 Pik-p Pik-h Total genes
IR31892-100-3-3-3 1a 1 1 0a 0 1 0 1 1 0 0 6
M2-9-14UL 1 1 1 0 0 0 0 1 0 0 0 4
C5216-B-3-1-1 1 1 1 0 0 0 0 1 1 0 0 5
Chang B8 1 1 1 0 0 0 1 1 0 1 0 6
Chang A5 1 1 1 0 0 0 1 1 1 0 1 7
Yuetai 13 1 1 1 0 0 0 1 1 1 1 0 7
Huanglizhan 1 1 0 0 0 0 0 1 1 0 0 4
Yebahuangzhan 1 1 0 0 0 0 1 1 1 1 1 7
Taiaosimiao 1 1 1 0 0 0 1 1 0 1 0 6
Fengsizhan 1 1 1 1 0 0 0 1 1 0 0 1 6
Baitaixiangzhan 1 1 1 0 0 0 1 1 0 0 0 5
IR76479-48-1-3-1 1 1 0 0 0 0 0 1 1 0 0 4
IR72903-99-2-3-2 1 1 1 0 0 0 0 1 0 1 0 5
Zhaitang 1 1 0 0 0 0 0 0 1 0 0 3
Xuxiaoqu 10 1 1 1 1 0 0 0 1 0 0 1 6
062A1 1 1 1 0 0 0 1 1 0 0 0 5
N11 1 1 1 1 0 0 0 1 0 0 1 6
A10 1 1 1 0 0 0 1 1 1 0 0 6
C3 1 1 1 0 0 0 1 1 1 0 1 7
Yunjing 136 1 1 1 1 0 0 0 1 0 0 0 5
Zhongle 8610 1 1 1 0 0 1 0 1 0 0 1 6
41 1 1 1 1 0 0 0 1 0 0 1 6
43 1 1 1 1 0 0 0 1 0 0 0 5
488 1 1 1 0 0 0 1 1 0 0 1 6
6003 1 1 1 1 1 1 0 1 0 1 1 9
6004 1 1 1 1 0 0 0 1 0 0 1 6
6020 1 1 1 1 0 0 0 1 0 0 0 5
6021 1 1 1 1 0 0 0 1 0 0 0 5
6181 1 1 1 1 0 0 0 1 0 1 1 7
237-1 1 1 1 1 0 0 0 1 0 1 1 7
237-2 1 1 1 1 1 1 0 1 0 1 1 9
1579 1 1 1 0 1 0 0 1 1 0 0 6
R gene frequency (%) 100.0 100.0 87.5 37.5 9.4 12.5 34.4 96.9 34.4 28.1 43.8
a, ‘1’ represents presence of amplicon and ‘0’ represents absence of amplicon.

Table 2 Single nucleotide polymorphisms and sequence-tagged site markers associated with eleven blast resistance genes in 32 rice germplasms.

Accession Pi-d2 Pi-z Piz-t Pi-9 Pi-36 Pi-37 Pi5 Pi-b Pi-ta2 Pik-p Pik-h Total genes
IR31892-100-3-3-3 1a 1 1 0a 0 1 0 1 1 0 0 6
M2-9-14UL 1 1 1 0 0 0 0 1 0 0 0 4
C5216-B-3-1-1 1 1 1 0 0 0 0 1 1 0 0 5
Chang B8 1 1 1 0 0 0 1 1 0 1 0 6
Chang A5 1 1 1 0 0 0 1 1 1 0 1 7
Yuetai 13 1 1 1 0 0 0 1 1 1 1 0 7
Huanglizhan 1 1 0 0 0 0 0 1 1 0 0 4
Yebahuangzhan 1 1 0 0 0 0 1 1 1 1 1 7
Taiaosimiao 1 1 1 0 0 0 1 1 0 1 0 6
Fengsizhan 1 1 1 1 0 0 0 1 1 0 0 1 6
Baitaixiangzhan 1 1 1 0 0 0 1 1 0 0 0 5
IR76479-48-1-3-1 1 1 0 0 0 0 0 1 1 0 0 4
IR72903-99-2-3-2 1 1 1 0 0 0 0 1 0 1 0 5
Zhaitang 1 1 0 0 0 0 0 0 1 0 0 3
Xuxiaoqu 10 1 1 1 1 0 0 0 1 0 0 1 6
062A1 1 1 1 0 0 0 1 1 0 0 0 5
N11 1 1 1 1 0 0 0 1 0 0 1 6
A10 1 1 1 0 0 0 1 1 1 0 0 6
C3 1 1 1 0 0 0 1 1 1 0 1 7
Yunjing 136 1 1 1 1 0 0 0 1 0 0 0 5
Zhongle 8610 1 1 1 0 0 1 0 1 0 0 1 6
41 1 1 1 1 0 0 0 1 0 0 1 6
43 1 1 1 1 0 0 0 1 0 0 0 5
488 1 1 1 0 0 0 1 1 0 0 1 6
6003 1 1 1 1 1 1 0 1 0 1 1 9
6004 1 1 1 1 0 0 0 1 0 0 1 6
6020 1 1 1 1 0 0 0 1 0 0 0 5
6021 1 1 1 1 0 0 0 1 0 0 0 5
6181 1 1 1 1 0 0 0 1 0 1 1 7
237-1 1 1 1 1 0 0 0 1 0 1 1 7
237-2 1 1 1 1 1 1 0 1 0 1 1 9
1579 1 1 1 0 1 0 0 1 1 0 0 6
R gene frequency (%) 100.0 100.0 87.5 37.5 9.4 12.5 34.4 96.9 34.4 28.1 43.8
a, ‘1’ represents presence of amplicon and ‘0’ represents absence of amplicon.
Fig. 1. Distribution of blast disease score of 32 resistant rice germplasms.

Fig. 1. Distribution of blast disease score of 32 resistant rice germplasms.

参考文献 32

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