Immune Sensor Xa21 Regulates Bacterial Leaf Blight Infection in Seven Rice Cultivars from Myanmar

doi:10.1016/j.rsci.2024.06.008

Abstract

Nay Chi Aye, Rizwana Begum Syed Nabi, Muhammad Shahid, Nkulu Kabange Rolly, Rupesh Tayade, Lee-Bong Choon, Adil Hussain, Byung-Wook Yun. Immune Sensor Xa21 Regulates Bacterial Leaf Blight Infection in Seven Rice Cultivars from Myanmar[J]. Rice Science, 2024, 31(6): 634-637.

Figures/Tables 1

Fig. 1. Response of rice cultivars to Xanthomonas oryzae pv. oryzae (Xoo) infection. A-B, Percentage of lesion length of rice cultivars in response to infection by Xoo strains K1 (A) and K3a (B). C, PCR-based detection of variations in the expression of bacterial leaf blight resistance (R) genes (Xa2, Xa4, xa5, xa13, and Xa21) in 11 rice cultivars. ‘+’ and ‘-’ represent the presence and absence of the corresponding Xa gene, respectively. D-G, Transcript accumulation of resistance genes OsJAZ8 (D), OsPR1a (E), OsPR10b (F), and OsWRKY45 (G). Plants were inoculated with Xoo K1 and K3a strains by leaf clipping method. Leaves of the control plants were clipped by dipping the scissors in autoclaved distilled water. Leaf samples were collected for qRT-PCR after 4 d of inoculation. The rice actin gene (ACT1) was used as the internal control. Jinbaek and Nampyeong are bacterial leaf blight-resistant and -susceptible japonica rice lines, while Tetep and IR661 are bacterial leaf blight-resistant and -susceptible indica rice lines. Data in A and B are Mean ± SD (n = 27), and different lowercase letters indicate significant differences at the 0.05 level according to the Duncan’s multiple range test. Data in D-G are Mean ± SE (n = 3). *, **, ***, and **** indicate significant differences at the 0.05, 0.01, 0.001, and 0.0001 levels, respectively, followed by the Šídák’s multiple comparisons test.

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