Increasing Fatty Acids in Rice Root Improves Silence of Rice Seedling to Salt Stress

doi:10.1016/j.rsci.2019.01.005

Abstract

Ling Liu, Jin Chen, Yanning Tan, Tianshun Zhou, Ning Ouyang, Jia Zeng, Dingyang Yuan, Meijuan Duan. Increasing Fatty Acids in Rice Root Improves Silence of Rice Seedling to Salt Stress[J]. Rice Science, 2019, 26(6): 339-342.

Figures/Tables 4

Fig. 1. Property of fatty acids in roots of rice seedlings under salt stress.A, Root lengths of wild type rice treated with different concentrations of NaCl. B, Thin layer chromatography (TLC) analysis of fatty acids in rice roots under different NaCl concentrations. The arrow shows triacylglycerol. C, Seed germination and root elongation. D, TLC analysis of fatty acids in rice roots treated with or without 100 mmol/L NaCl. E, Gas cholography analysis of Nipp and DGAT1 rice roots treated with or without 100 mmol/L NaCl. F, Fatty acid ratio in rice roots with or without 100 mmmol/L NaCl treatment. Nipp, Nipponbare.**, P < 0.01 by the Student’s t test with the comparison between DGAT1 and Nipp rice roots treated with or without NaCl. Values are Mean ± SD (n = 3).

Supplemental Fig. 1 Expression level of AtDGAT1 and fatty acids contents in DGAT1 rice root supplied with different concentrations of sucrose.A, Expression analysis of AtDGAT1 in rice root corresponding to different concentrations of sucrose. B, Gas chorography analysis of triacyglycerol (TAG) content in DGAT1 rice root treated with 30 mmol/L sucrose.**, P < 0.01 by the student's t-test compared with 0 mmol/L sucrose in Supplemental Fig. 1-A and compared DGAT1 rice with Nipp in Supplemental Fig. 1-B. Values are Mean ± SD (n = 3).

Supplemental Table 1 Primers used in this study.

Primer name	Gene name	Primer sequence (5'-3')
OsLEA-3F	OsLEA-3	AGCGTCCTCCAACAGGCGAG
OsLEA-3R	OsLEA-3	GTGATCCCTCGCCGTCGTCT
OsRMCF	OsRMC	TCGGAGGTGTACCCGTTCTACA
OsRMCR	OsRMC	ACTCTTAATTTGTGCCATTTTATTCTAGCT
OsDREB2AF	OsDREB2	AAAAGCGATGGCCCTGATTC
OsDREB2AR	OsDREB2	TTGGCTGGCGCTTTCCT
OsRAB16BF	OsRab16b	CACACCACAGCAAGAGCTAAGTG
OsRAB16BR	OsRab16b	TGGTGCTCCATCCTGCTTAAG
OsWRI1F	OSWRI1	AAGCCTCTGG AGCCCTTGCT
OsWRI1R	OSWRI1	CCGAGCTATCCTTGCTGCCT
FAD2F	FAD2	CCACCTCTTCTCCACCATGC
FAD2R	FAD2	CTCCTTGGCCTCGCGCCATG
FATAF	FATA	GCACCGCACC CAGAAGAGCA
FATAR	FATA	CGCCCACGGTTGATCTCGTT
PKF	PK	GAGTGAAGCA ACGTCTGGCA
PKR	PK	CTCTGAACAAGGGTCACGTAG
ACCF	ACC	CTATGAGAATCAACTCGAGG
ACCR	ACC	GCTCCCTCTTTGAAGGATTCA
CALEOSINF	Caleosin	TTCGGATGGTTCGCGGCGAA
CALEOSINR	Caleosin	TCGTACATGCGCCGGATGGC
OsActin10F	OsActin11	AGCCAAGAGGAGCTGTTATCG
OsActin10R	OsActin11	GATAACAGATAGGCCGGTTGAA
HPTIIF	HPTII	GTCAGTGCGTCCGTCGCGCAG
HPTIIR	HPTII	GCTTCTGCGGGCGATTTGTGTA
OsAGPS2bpF		GTCGACCAACTATTGATGACGCTAAG
OsAGPS2bpR	OsAGPS2b	GGCGCGCCCCTGCCTCGTGTCGTGTGGC
AtDGAT1F	AtDGAT1	GGCGCGCCATGGCGATTT TGGATTCTGC
AtDGAT1R		GTCGACTCATGACATCGATCCTTTTC

Fig. 2. qRT-PCR analysis of genes responsible for salt stress and fatty acids synthesis.A to J, Relative expression levels (REL) of genes in rice roots under different NaCl treatments. K to T, Relative expression levels in Nipponbare (Nipp) and DGAT1 rice roots treated with 100 mmol/L NaCl. Values are Mean ± SD (n = 3). Actin10 was used as a housekeeping gene.

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