A β-ketoacyl-CoA Synthase OsCUT1 Confers Increased Drought Tolerance in Rice
#These authors contributed equally to this work
Received date: 2021-08-24
Accepted date: 2021-12-13
Online published: 2021-12-15
Drought stress is one of the major environmental factors affecting crop growth and productivity. Cuticular wax plays essential roles in protecting plants from environmental stress via forming a hydrophobic barrier on leaf epidermis. In this study, we analyzed nine members (OsCUT1?OsCUT9) of β-ketoacyl-CoA synthase, the rate-limiting key enzyme for cuticular wax synthesis in rice by homology search and domain prediction. The expression levels of OsCUT genes under different abiotic stresses were investigated and OsCUT1 down-regulated by abiotic stress was selected for further function validation. Compared to the wild type, overexpression of OsCUT1 (OX-OsCUT1) exhibited significantly increased drought resistance. Epicuticular wax was increased on the leaf surface of OX-OsCUT1 and the chlorophyll leaching experiment showed that the cuticular permeability was decreased in the OX-OsCUT1 plants. Moreover, overexpression of OsCUT1 didn’t result in the significant changes of major agronomic traits. In total, these results suggested that OsCUT1 is a promising gene for engineering rice plants with enhanced drought tolerance.
Key words： rice; β-ketoacyl-CoA synthase; drought tolerance; cuticular wax; OsCUT1
Gao Xiuying, Zhang Ye, Zhang Hongsheng, Huang Ji . A β-ketoacyl-CoA Synthase OsCUT1 Confers Increased Drought Tolerance in Rice[J]. Rice Science, 2022 , 29(4) : 353 -362 . DOI: 10.1016/j.rsci.2021.12.009
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