Fluorescence Detection of Bacterial Pathogens in Rice by Recombinant Polymerase Amplification Based on Self-Heating Paper Chip

Yang Ning, Fu Jiuju, Wang Aiying, Liu Shuhua, Chang Kangpeng, Song Wei, Tao Shoulong, Mao Hanping, Zhang Xiaodong, Xu Lijia

doi:10.1016/j.rsci.2025.01.001

2025 , Vol. 32 >Issue 2: 131 - 136

DOI: https://doi.org/10.1016/j.rsci.2025.01.001

Letters

Fluorescence Detection of Bacterial Pathogens in Rice by Recombinant Polymerase Amplification Based on Self-Heating Paper Chip

Expand

¹School of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, China
²State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China
³School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013, China
⁴College of Mechanical and Electrical Engineering, Sichuan Agricultural University, Ya'an 625000, China

Received date: 2024-09-10

Accepted date: 2024-12-02

Online published: 2025-04-14

Fold

Cite this article

Yang Ning, Fu Jiuju, Wang Aiying, Liu Shuhua, Chang Kangpeng, Song Wei, Tao Shoulong, Mao Hanping, Zhang Xiaodong, Xu Lijia . Fluorescence Detection of Bacterial Pathogens in Rice by Recombinant Polymerase Amplification Based on Self-Heating Paper Chip[J]. Rice Science, 2025 , 32(2) : 131 -136 . DOI: 10.1016/j.rsci.2025.01.001

References

[1]	Aghababaie M, Foroushani E S, Changani Z, et al. 2023. Recent advances in the development of enzymatic paper-based microfluidic biosensors. Biosens Bioelectron, 226: 115131.
[2]	Ahn H, Batule B S, Seok Y, et al. 2018. Single-step recombinase polymerase amplification assay based on a paper chip for simultaneous detection of multiple foodborne pathogens. Anal Chem, 90(17): 10211-10216.
[3]	Banerjee A, Bag M K, Chandra A K, et al. 2023a. Development and application of recombinase polymerase amplification for rapid detection of rice false smut pathogen (Ustilaginoidea virens). Crop Prot, 167: 106204.
[4]	Banerjee A, Bharti S, Kumar J, et al. 2023b. Recombinase polymerase amplification based rapid detection of aroma gene in rice. Rice Sci, 30(2): 96-99.
[5]	Bao M D, Chen Q, Xu Z H, et al. 2021. Challenges and opportunities for clustered regularly interspaced short palindromic repeats based molecular biosensing. ACS Sens, 6(7): 2497-2522.
[6]	Eady M, Park B, Hinton A. 2020. Rapid identification of Campylobacter strains cultured under aerobic incubation using hyperspectral microscope imaging. J Food Prot, 83(3): 405-411.
[7]	Erkocyigit B A, Ozufuklar O, Yardim A, et al. 2023. Biomarker detection in early diagnosis of cancer: Recent achievements in point-of-care devices based on paper microfluidics. Biosensors, 13(3): 387.
[8]	Kaymaz S V, Akar U, Elitas M. 2021. Nucleic acid extraction methods and technologies for on-site plant-pathogen diagnostics. ACS Agric Sci Technol, 1(6): 581-585.
[9]	Kim H J, Kim Y, Park S J, et al. 2018. Development of colorimetric paper sensor for pesticide detection using competitive-inhibiting reaction. BioChip J, 12(4): 326-331.
[10]	Lee H, Yi S Y, Kwon J S, et al. 2021. Rapid and highly sensitive pathogen detection by real-time DNA monitoring using a nanogap impedimetric sensor with recombinase polymerase amplification. Biosens Bioelectron, 179: 113042.
[11]	Lee S H, Park S M, Kim B N, et al. 2019. Emerging ultrafast nucleic acid amplification technologies for next-generation molecular diagnostics. Biosens Bioelectron, 141: 111448.
[12]	Liu H X, Zhou X M, Liu W P, et al. 2016. Paper-based bipolar electrode electrochemiluminescence switch for label-free and sensitive genetic detection of pathogenic bacteria. Anal Chem, 88(20): 10191-10197.
[13]	Magro L, Jacquelin B, Escadafal C, et al. 2017. Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics. Sci Rep, 7(1): 1347.
[14]	Rabello L G,da Conceição Ribeiro R C. 2021. A novel vermiculite/ vegetable polyurethane resin-composite for thermal insulation eco-brick production. Compos Part B Eng, 221: 109035.
[15]	Rohrman B A, Richards-Kortum R R. 2012. A paper and plastic device for performing recombinase polymerase amplification of HIV DNA. Lab Chip, 12(17): 3082-3088.
[16]	Roy S, Mohd-Naim N F, Safavieh M, et al. 2017. Colorimetric nucleic acid detection on paper microchip using loop mediated isothermal amplification and crystal violet dye. ACS Sens, 2(11): 1713-1720.
[17]	Seok Y, Joung H A, Byun J Y, et al. 2017. A paper-based device for performing loop-mediated isothermal amplification with real- time simultaneous detection of multiple DNA targets. Theranostics, 7(8): 2220-2230.
[18]	Shasmita, Samal P, Mohapatra P K, et al. 2021. Improved photosystem II and defense enzymes activity in rice (Oryza sativa) by biopriming against Xanthomonas oryzae pv. oryzae. Funct Plant Biol, 48(3): 298-311.
[19]	Shruthi U, Nagaveni V, Raghavendra B K. 2019. A review on machine learning classification techniques for plant disease detection. In: 2019 5th International Conference on Advanced Computing & Communication Systems (ICACCS). March 15-16, 2019, Coimbatore, India: IEEE: 281-284.
[20]	Somvanshi S B, Ulloa A M, Zhao M, et al. 2022. Microfluidic paper-based aptasensor devices for multiplexed detection of pathogenic bacteria. Biosens Bioelectron, 207: 114214.
[21]	Tang R H, Yang H, Gong Y, et al. 2017. A fully disposable and integrated paper-based device for nucleic acid extraction, amplification and detection. Lab Chip, 17(7): 1270-1279.
[22]	Thepbandit W, Papathoti N K, Daddam J R, et al. 2021. Identification of salicylic acid mechanism against leaf blight disease in Oryza sativa by SR-FTIR microspectroscopic and docking studies. Pathogens, 10(6): 652.
[23]	Toth A, Bobok E. 2017. Basic equations of fluid mechanics and thermodynamics. In: Flow and Heat Transfer in Geothermal Systems. Amsterdam, the Netherlands: Elsevier: 21-55.
[24]	Wang Y Q, Ke Y Q, Liu W J, et al. 2020. A one-pot toolbox based on Cas12a/crRNA enables rapid foodborne pathogen detection at attomolar level. ACS Sens, 5(5): 1427-1435.
[25]	Weng S Z, Hu X J, Wang J H, et al. 2021. Advanced application of Raman spectroscopy and surface-enhanced Raman spectroscopy in plant disease diagnostics: A review. J Agric Food Chem, 69(10): 2950-2964.
[26]	Yang N, Wang P, Xue C Y, et al. 2018. A portable detection method for organophosphorus and carbamates pesticide residues based on multilayer paper chip. J Food Process Eng, 41(8): e12867.
[27]	Zhang J N, Yang Y, Feng X P, et al. 2020. Identification of bacterial blight resistant rice seeds using terahertz imaging and hyperspectral imaging combined with convolutional neural network. Front Plant Sci, 11: 821.
[28]	Zhu Z B, Li R, Zhang H W, et al. 2022. PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogens. Biosens Bioelectron, 204: 114076.
[29]	Zhuang J W, Zhao Z Y, Lian K, et al. 2022. SERS-based CRISPR/ Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods. Biosens Bioelectron, 207: 114167.

Options

Outlines

模态框（Modal）标题

Cite this article

References