Identification and Characterization of WAKg Genes Involved in Rice Disease Resistance and Yield
Received date: 2025-02-02
Accepted date: 2025-03-16
Online published: 2025-04-27
The wall-associated kinases (WAKs) play a crucial role in rice resistance, but their relationship to yield-related traits remains poorly understood. In this study, we analyzed the rice wall-associated kinase galacturonan-binding (WAKg) gene family and evaluated its association with both disease resistance and grain yield. A total of 108 OsWAKg genes were identified in rice. Promoter cis-element analysis revealed that the promoter regions of OsWAKg genes contain abundant resistance- and hormone-related elements. Induced expression analysis of 18 OsWAKg genes highly expressed in both rice leaves and roots showed that 14 genes were pathogen-induced, 9 were induced by development-related hormones, and 8 were responded to both stimuli. Transgenic validation confirmed that OsWAKg16 and OsWAKg52 positively regulate rice disease resistance and yield. Moreover, OsWAKg52 regulates rice disease resistance through multiple pattern-triggered immunity responses. These findings demonstrate that OsWAKgs significantly contribute to the coordinated regulation of disease resistance and grain yield, providing new insights into rice WAKg gene family and potential genetic resources for synergistic crop improvement.
Ayaz Ahmad, Cheng Mingxing, Guo Yu, Luo Xiong, Yang Zihan, Liu Manman, Yuan Huanran, Li Qiancheng, Li Shaoqing, Fan Fengfeng . Identification and Characterization of WAKg Genes Involved in Rice Disease Resistance and Yield[J]. Rice Science, 2025 , 32(5) : 673 -684 . DOI: 10.1016/j.rsci.2025.04.011
| [1] | Bailey T L, Boden M, Buske F A, et al. 2009. MEME SUITE: Tools for motif discovery and searching. Nucleic Acids Res, 37: W202-W208. |
| [2] | Bolser D M, Staines D M, Perry E, et al. 2017. Ensembl plants: Integrating tools for visualizing, mining, and analyzing plant genomic data. Methods Mol Biol, 1533: 1-31. |
| [3] | Cai W G, Hong J, Liu Z Y, et al. 2023. A receptor-like kinase controls the amplitude of secondary cell wall synthesis in rice. Curr Biol, 33(3): 498-506.e6. |
| [4] | Camacho C, Coulouris G, Avagyan V, et al. 2009. BLAST+: Architecture and applications. BMC Bioinformatics, 10: 421. |
| [5] | Chen C J, Chen H, Zhang Y, et al. 2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Mol Plant, 13(8): 1194-1202. |
| [6] | Chen R Z, Deng Y W, Ding Y L, et al. 2022. Rice functional genomics: Decades’ efforts and roads ahead. Sci China: Life Sci, 65(1): 33-92. |
| [7] | Chen Y P, Dan Z W, Li S Q. 2024. GROWTH REGULATING FACTOR 7-mediated arbutin metabolism enhances rice salt tolerance. Plant Cell, 36(8): 2834-2850. |
| [8] | Cheng M X, Yuan H R, Wang R H, et al. 2023. Identification and characterization of BES1 genes involved in grain size development of Oryza sativa L. Int J Biol Macromol, 253(Pt 6): 127327. |
| [9] | Dai Z Y, Tan J, Zhou C, et al. 2019. The OsmiR396-OsGRF8-OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa). Plant Biotechnol J, 17(8): 1657-1669. |
| [10] | de Oliveira L F V, Christoff A P, de Lima J C, et al. 2014. The wall-associated kinase gene family in rice genomes. Plant Sci, 229: 181-192. |
| [11] | Delteil A, Gobbato E, Cayrol B, et al. 2016. Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus. BMC Plant Biol, 16: 17. |
| [12] | Deng Y W, Zhai K R, Xie Z, et al. 2017. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance. Science, 355: 962-965. |
| [13] | Fan F F, Liu M M, Li N N, et al. 2023. Gain-of-function allele of HPY1 coordinates source and sink to increase grain yield in rice. Sci Bull, 68(19): 2155-2159. |
| [14] | Finn R D, Clements J, Eddy S R. 2011. HMMER web server: Interactive sequence similarity searching. Nucleic Acids Res, 39: W29-W37. |
| [15] | Gao F, Wang K, Liu Y, et al. 2016. Blocking miR396 increases rice yield by shaping inflorescence architecture. Nat Plants, 2: 15196. |
| [16] | Goodstein D M, Shu S Q, Howson R, et al. 2012. Phytozome: A comparative platform for green plant genomics. Nucleic Acids Res, 40: D1178-D1186. |
| [17] | Guo J P, Wang H Y, Guan W, et al. 2023. A tripartite rheostat controls self-regulated host plant resistance to insects. Nature, 618: 799-807. |
| [18] | Harkenrider M, Sharma R, de Vleesschauwer D, et al. 2016. Overexpression of rice wall-associated kinase 25 (OsWAK25) alters resistance to bacterial and fungal pathogens. PLoS One, 11(1): e0147310. |
| [19] | Huluka W, Kumsa L. 2022. Analysis of rice (Oryza sativa L. ssp. japonica) wall associated receptor-like protein kinase gene’s promoter region and regulatory elements. Curr Plant Biol, 31: 100254. |
| [20] | Krzywinski M, Schein J, Birol I, et al. 2009. Circos: An information aesthetic for comparative genomics. Genome Res, 19(9): 1639-1645. |
| [21] | Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol, 33(7): 1870-1874. |
| [22] | Li H, Zhou S Y, Zhao W S, et al. 2009. A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice blast disease resistance. Plant Mol Biol, 69(3): 337-346. |
| [23] | Li W T, Zhu Z W, Chern M, et al. 2017. A natural allele of a transcription factor in rice confers broad-spectrum blast resistance. Cell, 170(1): 114-126.e15. |
| [24] | Liu Y, Zhang X, Yuan G X, et al. 2021. A designer rice NLR immune receptor confers resistance to the rice blast fungus carrying noncorresponding avirulence effectors. Proc Natl Acad Sci USA, 118: e2110751118. |
| [25] | Long S P, Marshall-Colon A, Zhu X G. 2015. Meeting the global food demand of the future by engineering crop photosynthesis and yield potential. Cell, 161(1): 56-66. |
| [26] | Long W X, Li N W, Jin J, et al. 2023. Resequencing-based QTL mapping for yield and resistance traits reveals great potential of Oryza longistaminata in rice breeding. Crop J, 11(5): 1541-1549. |
| [27] | Rao Y C, Li Y Y, Qian Q. 2014. Recent progress on molecular breeding of rice in China. Plant Cell Rep, 33(4): 551-564. |
| [28] | Sipahi H, Whyte T D, Ma G, et al. 2022. Genome-wide identification and expression analysis of wall-associated kinase (WAK) gene family in Cannabis sativa L. Plants, 11(20): 2703. |
| [29] | Subramanian B, Gao S H, Lercher M J, et al. 2019. Evolview v3: A webserver for visualization, annotation, and management of phylogenetic trees. Nucleic Acids Res, 47(W1): W270-W275. |
| [30] | Verica J A, Chae L, Tong H Y, et al. 2003. Tissue-specific and developmentally regulated expression of a cluster of tandemly arrayed cell wall-associated kinase-like kinase genes in Arabidopsis. Plant Physiol, 133(4): 1732-1746. |
| [31] | Wang J, Zhou L, Shi H, et al. 2018. A single transcription factor promotes both yield and immunity in rice. Science, 361: 1026-1028. |
| [32] | Wang Y, Yue J L, Yang N, et al. 2023. An ERAD-related ubiquitin-conjugating enzyme boosts broad-spectrum disease resistance and yield in rice. Nat Food, 4(9): 774-787. |
| [33] | Wang Y H, Xue Y B, Li J Y. 2005. Towards molecular breeding and improvement of rice in China. Trends Plant Sci, 10(12): 610-614. |
| [34] | Wang Y P, Tang H B, Debarry J D, et al. 2012. MCScanX: A toolkit for detection and evolutionary analysis of gene synteny and collinearity. Nucleic Acids Res, 40(7): e49. |
| [35] | Yang J, Zhao X Y, Sun J, et al. 2010. A novel protein Com1 is required for normal conidium morphology and full virulence in Magnaporthe oryzae. Mol Plant Microbe Interact, 23(1): 112-123. |
| [36] | Yuan H R, Cheng M X, Fan F F, et al. 2024a. OsGRF6-OsYUCCA1/ OsWRKY82 signaling cascade upgrade grain yield and bacterial blight resistance in rice. Adv Sci, 11: e2407733. |
| [37] | Yuan H R, Cheng M X, Wang R H, et al. 2024b. miR396b/GRF6 module contributes to salt tolerance in rice. Plant Biotechnol J, 22(8): 2079-2092. |
| [38] | Yue Z L, Liu N, Deng Z P, et al. 2022. The receptor kinase OsWAK11 monitors cell wall pectin changes to fine-tune brassinosteroid signaling and regulate cell elongation in rice. Curr Biol, 32(11): 2454-2466.e7. |
| [39] | Zhang F, Fang H, Wang M, et al. 2022. APIP5 functions as a transcription factor and an RNA-binding protein to modulate cell death and immunity in rice. Nucleic Acids Res, 50(9): 5064-5079. |
| [40] | Zhang S B, Chen C, Li L, et al. 2005. Evolutionary expansion, gene structure, and expression of the rice wall-associated kinase gene family. Plant Physiol, 139(3): 1107-1124. |
| [41] | Zhang Z Q, Ma W Y, Ren Z Y, et al. 2021. Characterization and expression analysis of wall-associated kinase (WAK) and WAK-like family in cotton. Int J Biol Macromol, 187: 867-879. |
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