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Optimizing Anther Culture for Doubled Haploid Breeding of Rice Using Yongyou 1540

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  • 1College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    2College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    3Agricultural Ecology Research Institute, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    4Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    5Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, China
    6Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
Wang Wenfei (wenfeiwang@fafu.edu.cn);
Yang Xuelian (xlyang@fafu.edu.cn)

Received date: 2025-06-16

  Accepted date: 2025-08-21

  Online published: 2026-02-03

Cite this article

Gan Yan, Xiao Kailing, Lin Jialing, Hong Yifei, Li Chuyi, Yu Hang, Zhang Long, Cui Shiyong, Sun Yanbo, Li Jinying, Chen Mingjiao, Chu Huangwei, Lin Wenxiong, Yang Xuelian, Wang Wenfei . Optimizing Anther Culture for Doubled Haploid Breeding of Rice Using Yongyou 1540[J]. Rice Science, 2026 , 33(1) : 5 -8 . DOI: 10.1016/j.rsci.2025.08.011

References

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[2] Chen H F, Yao F F, Yang Y C, et al. 2023. Progress and challenges of rice ratooning technology in Fujian Province, China. Crop Environ, 2(3): 121-125.
[3] Eliby S, Bekkuzhina S, Kishchenko O, et al. 2022. Developments and prospects for doubled haploid wheat. Biotechnol Adv, 60: 108007.
[4] Jacquier N M A, Gilles L M, Martinant J P, et al. 2021. Maize in planta haploid inducer lines: A cornerstone for doubled haploid technology. Methods Mol Biol, 2288: 25-48.
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[9] Mheni N T, Kilasi N, Quiloy F A, et al. 2024. Breeding rice for salinity tolerance and salt-affected soils in Africa: A review. Cogent Food Agric, 10: 2327666.
[10] Mishra R, Rao G J N, Rao R N, et al. 2015. Development and characterization of elite doubled haploid lines from two indica rice hybrids. Rice Sci, 22(6): 290-299.
[11] Nguyen H, Chen X Y, Jiang M, et al. 2016. Development and molecular characterization of a doubled haploid population derived from a hybrid between japonica rice and wide compatible indica rice. Breed Sci, 66(4): 552-559.
[12] Oladosu Y, Rafii M Y, Samuel C, et al. 2019. Drought resistance in rice from conventional to molecular breeding: A review. Int J Mol Sci, 20(14): 3519.
[13] Sen A, Beser N. 2022. Gum arabic application improve anther culture efficiency in rice. Fresenius Environm Bull, 31(9): 9867-9877.
[14] Sun J, Ma L X. 2017. Related research on the suitable conditions of rice anther culture. North Rice, 47(1): 35-37. (in Chinese with English abstract)
[15] Till B J, Hofinger B J, Sen A, et al. 2017. A protocol for validation of doubled haploid plants by enzymatic mismatch cleavage. In: Jankowicz-CieslakJ, TaiT, KumlehnJ, et al. Biotechnologies for Plant Mutation Breeding. Cham, Swizerland: Springer: 253-262.
[16] Tripathy S K, Swain D, Mohapatra P M, et al. 2019. Exploring factors affecting anther culture in rice (Oryza sativa L.). J Appl Biol Biotechnol, 7(2): 87-92.
[17] Weyen J. 2021. Applications of doubled haploids in plant breeding and applied research. In: Sugui-Simarro JM. Methods in Molecular Biology: Doubled Haploid Technology, Volume 1. Humana: New York, USA: 23-39.
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