
Engineering Prime Editing System for Concurrent Gene Knockout and Point Mutation Induction (KAPSi) in Rice
Received date: 2025-12-29
Accepted date: 2026-02-09
Online published: 2026-06-02
Liu Xiaoshuang, Ma Chong, Wang Dongmei, Xu Youshen, Qin Ruiying, Li Juan, Xu Rongfang, Wei Pengcheng . Engineering Prime Editing System for Concurrent Gene Knockout and Point Mutation Induction (KAPSi) in Rice[J]. Rice Science, 2026 , 33(3) : 277 -281 . DOI: 10.1016/j.rsci.2026.02.008
| [1] | Anzalone A V, Randolph P B, Davis J R, et al. 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576: 149-157. |
| [2] | Chen K L, Wang Y P, Zhang R, et al. 2019. CRISPR/Cas genome editing and precision plant breeding in agriculture. Annu Rev Plant Biol, 70: 667-697. |
| [3] | Duan P G, Ni S, Wang J M, et al. 2015. Regulation of OsGRF4 by OsmiR396 controls grain size and yield in rice. Nat Plants, 2(1): 15203. |
| [4] | Gao C X. 2021. Genome engineering for crop improvement and future agriculture. Cell, 184(6): 1621-1635. |
| [5] | Gupta A, Liu B, Raza S, et al. 2024. Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice. Plant Commun, 5(2): 100741. |
| [6] | Jinek M, Chylinski K, Fonfara I, et al. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337: 816-821. |
| [7] | Komor A C, Kim Y B, Packer M S, et al. 2016. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature, 533: 420-424. |
| [8] | Kuscu C, Parlak M, Tufan T, et al. 2017. CRISPR-STOP gene silencing through base-editing-induced nonsense mutations. Nat Methods, 14(7): 710-712. |
| [9] | Li C, Zong Y, Jin S, et al. 2020. SWISS: Multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds. Genome Biol, 21(1): 141. |
| [10] | Liu T T, Zou J P, Yang X, et al. 2023. Development and application of prime editing in plants. Rice Sci; 30(6): 509-522. |
| [11] | Liu Y Z, Gao Y K, Gao Y H, et al. 2019. Targeted deletion of floral development genes in Arabidopsis with CRISPR/Cas 9 using the RNA endoribonuclease Csy4 processing system. Hortic Res, 6: 99. |
| [12] | Ni P, Zhao Y D, Zhou X M, et al. 2023. Efficient and versatile multiplex prime editing in hexaploid wheat. Genome Biol, 24(1): 156. |
| [13] | Verma A, Prakash G, Ranjan R, et al. 2021. Silencing of an ubiquitin ligase increases grain width and weight in indica rice. Front Genet, 11: 600378. |
| [14] | Xu R F, Li J, Liu X S, et al. 2020. Development of plant prime-editing systems for precise genome editing. Plant Commun, 1(3): 100043. |
| [15] | Zhu H C, Li C, Gao C X. 2020. Applications of CRISPR-Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol, 21(11): 661-677. |
| [16] | Zou J P, Meng X B, Hong Z Y, et al. 2025. Cas9-PE: A robust multiplex gene editing tool for simultaneous precise editing and site-specific random mutation in rice. Trends Biotechnol, 43(2): 433-446. |
/
| 〈 |
|
〉 |