Avoidance of Linkage Drag Between Blast Resistance Gene and the QTL Conditioning Spikelet Fertility Based on Genotype Selection Against Heading Date in Rice

Liu Wen-qiang, Fan Ye-yang, Chen Jie, Shi Yong-feng, Wu Jian-li

doi:10.1016/S1672-6308(08)60052-9

2009 , Vol. 16 >Issue 1: 21 - 26

DOI: https://doi.org/10.1016/S1672-6308(08)60052-9

Research Paper

Avoidance of Linkage Drag Between Blast Resistance Gene and the QTL Conditioning Spikelet Fertility Based on Genotype Selection Against Heading Date in Rice

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Chinese National Center for Rice Improvement/State Key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received date: 2008-08-12

Online published: 2009-03-28

Supported by

the Science and Technology Program of Zhejiang Province, China (Grant No. 2005C24007) and National High Technology Research and Development Program of China (Grant Nos. 2006AA10Z1E8 and 2006AA100101).

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Abstract

Previous study showed that a linkage drag between a blast resistance gene Pi25(t) and QTLs conditioning spikelet fertility (qSF-6) and number of filled grains per panicle (qNFGP-6) was detected on the short arm of chromosome 6. A larger population was used for further verification, and the results confirmed the linkage drag between the blast resistance gene and QTL conditioning spikelet fertility, other than QTL conditioning number of filled grains per panicle. Breakdown or avoidance of the linkage drag could be achieved by selection against the genotype background of a heading-date gene (qHD-7) that resided in the region between RM2 and RM214 on chromosome 7. For further validation, two lines with almost identical genotypes on all chromosomal regions except the Pi25(t) region on chromosome 6 were chosen to develop a new population. The results showed that qSF-6 could be further subdivided into qSF-6-1 and qSF-6-2. When the genotype of the region between RM2 and RM214 was from rice variety Zhong 156, the linkage drag between Pi25(t) and qSF-6-2 was detected and the allele of qSF-6-2 from rice variety Gumei 2 reduced the spikelet fertility. When the genotype of the region between RM2 and RM214 was from Gumei 2, no linkage drag was detected. This indicates that the linkage drag between the blast resistance gene and the QTL conditioning spikelet fertility could be broken down or avoided under a certain background genotype selection against heading-date and provides a marker aided solution for high level of blast resistance and yield breeding in rice and other crops as well.

Key words： rice (Oryza sativa); linkage drag; blast resistance; quantitative trait locus; spikelet fertility; heading date

Cite this article

Liu Wen-qiang, Fan Ye-yang, Chen Jie, Shi Yong-feng, Wu Jian-li . Avoidance of Linkage Drag Between Blast Resistance Gene and the QTL Conditioning Spikelet Fertility Based on Genotype Selection Against Heading Date in Rice[J]. Rice Science, 2009 , 16(1) : 21 -26 . DOI: 10.1016/S1672-6308(08)60052-9

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