The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found during double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X (33.00%). Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.
DAI Xi-mei, YANG Xu, HUANG Qun-ce, QIN Guang-yong
. Observation on Double Fertilization and Early Embryonic Development in Autotetraploid Polyembryonic Rice[J]. Rice Science, 2009
, 16(2)
: 124
-130
.
DOI: 10.1016/S1672-6308(08)60068-2
1 Yuan L P. The strategy of hybrid rice breeding. Hybrid Rice, 1987, 1: 1–3. (in Chinese)
2 Asker S E, Jerling L. Apomixis in Plants. London: CRC Press, 1992: 109–111.
3 Liu Y S, Sun J S. An apomictic autotriploid line TAR identified in Oryza sativa. Acta Bot Sin, 1996, 38(11): 917– 920. (in Chinese with English abstract)
4 Yao J L, Cai D T, Ma P F, Wang Z A, Zhu H. Embryological study on the apomictic rice HDAE002. J Huazhong Agric Univ, 1994, 13(4): 339–343. (in Chinese with English abstract)
5 Liu Y S, Sun J S, Wang F W, Zhou K D. Cytoembryological studies on polyembryonic line SB-1 of Oryza sativa: Polyembryony and its origin. Acta Bot Sin, 1994, 36(11): 821–827. (in Chinese with English abstract)
6 Mu X J, Shi C G, Zhu Z Q, Cai X, Ni P C. Embryological observation on the apomictic rice ApIII (Shuang 13). Acta Bot Sin, 1996, 38(12): 969–971. (in Chinese)
7 Ye X L, Chen Z L, Li Y Q. Polyembryogenesis in apomixis parent IV of rice. J Trop & Subtrop Bot, 1994, 2(4): 67–72. (in Chinese with English abstract)
8 Liu X D, Lu Y G, Xu X B, Xu S X. Fertilization of different types of embryo sacs and its embryo formation in poly- embryonic rice strain APIV. Acta Bot Sin, 1997, 39(3): 214– 217. (in Chinese with English abstract)
9 Wu X J, Zhou K D. Embryogeny for polyembryo strain 9003 in rice (Oryza sativa L.). J Sichuan Univ: Nat Sci, 2003, 40(5): 966–969. (in Chinese with English abstract)
10 Xiao W. Embryonic and genetic study on twin-seedling in rice. [Dissertations of MSc]. Chengdu: Sichuan Agricultural University, 2006. (in Chinese with English abstract)
11 Yang H Y. A fluorescence staining-methyl salicylate clearing technique for demonstrating pollen nuclei. Acta Bot Sin, 1988, 30(3): 242-247.
12 Guo H B, Feng J H, Lu Y G, Liu X D. Abnormal structure of embryo sac in autotetraploid rice. Rice Sci, 2006, 13(4): 257– 264.
13 Wu S X, Cai Q G. Cytological studies of the double fertilization in rice. Acta Bot Sin, 1965, 13(2): 114–121. (in Chinese with English abstract)
14 Huang Q C, Xiang M C, Ji B J. Cytological observation on double fertilization of autotetraploid rice. J Xiangtan Normal Univ, 2000, 21(6): 84–87. (in Chinese with English abstract)
15 Wang L, Liu X D, Lu Y G, Feng J H, Xu X B, Xu S X. Endosperm development in autotetraploid rice. Rice Sci, 2005, 12(2): 83–91.
16 Zhang H H, Feng J H, Lu Y G. Observation on formation and development of autotetraploid rice embryo sac using laser scanning confocal microscope. J Chinese Electron Microscope Soc, 2003, 22(5): 380–384. (in Chinese with English abstract)
17 Yadegari R, Drews G N. Female gametophyte development. Plant Cell, 2004, 16: S133–S141.
18 Yu Z L, He J G, Dong J G, Wang X D, Wu Y J, Liu G F. Primary studies of mutational mechanism for rice induced by ion implantation. Anhui Agric Sci, 1989, 39(1): 12–16. (in Chinese)
19 Chen Y, Jiang B Y, Chen Y S, Ding X Z, Liu X H, Chen C S, Guo X Y, Yin G L. Formation of plasmid DNA strand breaks induced by low-energy ion beam: Indication of nuclear stopping effects. Radiat Environ Biophys, 1998, 37: 101–106.
20 Du Y H, Huang S H, Tan Z, Liu Y Z, Qiu G Y. Determination of DNA single-strand breaks by low-energy heavy ion and analysis of dose-effect curves. Chin Sci Bull, 1999, 44(8): 711–715.
21 Yu Z L. Ion beam application in genetic modification. IEEE Trans Plasma Sci, 2000, 28(1): 128–131.
