The effects of ethephon on the constitutive aerenchyma formation in roots were studied with a rice variety Yangdao 6 as material. The number of air spaces formed by disintegrated cells in mediopellis increased significantly with the rising ethephon concentrations, whereas superoxide dismutase (SOD) activity showed downward trends. Compared with the control, the expression levels of xyloglucan endotransglycosylase (XET) gene were markedly higher both at the apical 10 mm and distal parts of roots in 100 mg/L ethephon treated plants. The accumulation of XET was supposed to be associated with the aerenchyma development. Furthermore, earlier cortical cell death was observed under the ethephon treatments, and most of nuclei of cells at 4 mm from the root apex disintegrated with many Golgi apparatus, mitochondria and membrane- bound vesicles around the cell wall.
KONG Yu, WANG Zhong, CHEN Juan, PAN Xue-tong, LIU Da-tong, ZHANG Er-jin
. Effects of Ethephon on Aerenchyma Formation in Rice Roots[J]. Rice Science, 2009
, 16(3)
: 210
-216
.
DOI: 10.1016/S1672-6308(08)60081-5
1 Li Y C, Li Y S, Li S Q. Progress in research on injury to rice growth and development under submergence stress and mechanism of submergence tolerance. Chinese J Rice Sci, 1998, 12(suppl): 70–76. (in Chinese with English abstract)
2 Drew M C, Jackson M B, Giffard S. Ethylene-promoted adventitious rooting and development of cortical air spaces (aerenchyma) in roots may be adaptive responses to flooding in Zea mays L. Planta, 1979, 147(1): 83–88.
3 Jackson M B. Ethylene and responses of plants to soil waterlogging and submergence. Annu Rev Plant Physiol, 1985, 36: 145–174.
4 Wang G H, Wang H, Huang J C. Ethylene induced formation of maize cortex aerenchyma is involved to programmed cell death (PCD). J Central China Normal Univ: Nat Sci, 2000, 34(4): 463–467. (in Chinese with English abstract)
5 Gunawardena A H L A N, Pearce D M, Jackson M B, Hawes C R, Evans D E. Characterisation of programmed cell death during aerenchyma formation induced by ethylene or hypoxia in roots of maize. Planta, 2001, 212(2): 205–214.
6 He C J, Morgan P W, Drew M C. Transduction of an ethylene signal is required for cell death and lysis in the root cortex of maize during aerenchyma formation induced by hypoxia. Plant Physiol, 1996, 112(2): 463–472.
7 Wang W Q, Zheng Y Z, Mei H X, Zhang J S, Zhang F S. Anaerobic inducible expression of xyloglucan endotransglycosylase (XET) gene in sesame and wheat roots. J Agric Biotech, 2004, 12(3): 258–263. (in Chinese with English abstract)
8 Muhlenbock P, Plaszczyca M, Plaszczyca M, Mellerowicz E, Karpinski S. Lysigenous aerenchyma formation in Arabidopsis is controlled by lesion simulating disease. Plant Cell, 2007, 19(11): 3819–3830.
9 Colmer T D, Cox M C H, Voesenek L A C J. Root aeration in rice (Oryza sativa): Evaluation of oxygen, carbon dioxide, and ethylene as possible regulators of root acclimatizations. New Phytol, 2006, 170(4): 767–778.
10 Visser E J W, Bögemann G M. Aerenchyma formation in the wetland plant Juncus effusus is independent of ethylene. New Phytol, 2006, 171(2): 305–314.
11 Justin S H F W, Armstrong W. Evidence for the involvement of ethene in aerenchyma formation in adventitious roots of rice (Oryza sativa L.). New Phytol, 1991, 118: 49–62.
12 Jackson M B, Fenning T M, Jenkins W. Aerenchyma (gas- space) formation in adventitious roots of rice (Oryza sativa L.) is not controlled by ethylene or small partial pressures of oxygen. J Exp Bot, 1985, 36(10): 1566–1572.
13 Zou Q. Experiment Guide for Plant Physiology. Beijing: China Agricultural Press, 2000: 163–165. (in Chinese)
14 Genovesi V, Fornale S, Fry S C, Ruel K, Ferrer P, Encina A, Sonbol F M, Bosch J, Puigdomènech P, Rigau J, Caparrós- Ruiz D. ZmXTH1, a new xyloglucan endotransglucosylase/ hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana. J Exp Bot, 2008, 59(4): 875–889.
15 Saab I N, Sachs M M. A flooding-induced xyloglucan endo- transglycosylase homolog in maize is responsive to ethylene and associated with aerenchyma. Plant Physiol, 1996, 112(1): 385–391.
16 Wang J X, Yan X L, Pan R C. Relationship between adventitious root formation and plant hormones. Plant Physiol Comm, 2005, 41(2): 133–141. (in Chinese with English abstract)
17 Campbell R, Drew M C. Electron microscopy of gas space (aerenchyma) formation in adventitious roots of Zea mays L. subjected to oxygen shortage. Planta, 1983, 157(4): 350–357.
18 Drew M C, He C, Morgan P W. Programmed cell death and aerenchyma formation in roots. Trends Plant Sci, 2000, 5(3): 123–127.
19 Wei H P, Li R Q, Wang J B, Wu G L, Wu Y. Ultrastructural localization of cellulase activity in lytic root cortical cells during aerenchyma formation of flooded maize. Acta Agron Sin, 2006, 32(1): 84–87. (in Chinese with English abstract)
20 Kawai M, Samarajeewa1 P K, Barrero1 R A, Nishiguchi M, Uchimiya H. Cellular dissection of the degradation pattern of cortical cell death during aerenchyma formation of rice roots. Planta, 1998, 204: 277–287.
21 Kim H M, Ahn J W. Activation of the programmed cell death pathway by inhibition of proteasome function in plants. Biol Chem, 2003, 278(21): 19406–19415.
22 Voesenek L A C J, Colmer T D, Pierik R, Millenaar F F, Peeters A J M. How plants cope with complete submergence. New Phytol, 2006, 170(2): 213–226.
23 Tian M, Rao L B, Li J Y. Reactive oxygen species (ROS) and its physiological functions in plant cells. Plant Physiol Comm, 2005, 41(2): 235–241. (in Chinese with English abstract)
24 Fan M S, Zhang F S. Aerenchyma formation in plant and its physiological and ecological significance. Plant Physiol Comm, 2002, 38(6): 615–618. (in Chinese with English abstract)
