Research Paper

Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning (HEGS) System

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  • 1 National Institute of Agrobiological Sciences, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan; 2 Present address: School of Environmental Science, University of Shiga Prefecture, Hassaka, Hikone, Shiga 522-8533, Japan

Received date: 2009-06-09

  Online published: 2009-12-28

Supported by

the funds from CREST (Core research for evolutional science and technology) of the Japan Science and Technology Corporation, and BRAIN, Bio-oriented Technology Research Advancement Institution, Japan.

Abstract

High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS (high efficiency genome scanning) is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density (average interval 2.3 cM) map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.

Cite this article

Akifumi SHIMIZU, Shinji KAWASAKI . Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning (HEGS) System[J]. Rice Science, 2009 , 16(4) : 247 -253 . DOI: 10.1016/S1672-6308(08)60087-6

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