Two indica rice genotypes, viz. Milyang 46 and Zhenshan 97B differing in Cd accumulation and tolerance were used as materials in a hydroponic system consisting of four Cd levels (0, 0.1, 1.0 and 5.0 µmol/L) and three N levels (23.2, 116.0 and 232.0 mg/L) to study the effects of nitrogen status and nitrogen uptake capacity on Cd accumulation and tolerance in rice plants. N-efficient rice genotype, Zhenshan 97B, accumulated less Cd and showed higher Cd tolerance than N-inefficient rice genotype, Milyang 46. There was consistency between nitrogen uptake capacity and Cd tolerance in rice plants. Increase of N level in solution slightly increased Cd concentration in shoots but significantly increased in roots of both genotypes. Compared with the control at low N level, Cd tolerance in both rice genotypes could be significantly enhanced under normal N level, but no significant difference was observed between the Cd tolerances under normal N (116.0 mg/L) and high N (232.0 mg/L) conditions. The result proved that genotypic differences in Cd accumulation and toxicity could be, at least in part, attributed to N uptake capacity in rice plants.
DU Qin, CHEN Ming-xue, ZHOU Rong, CHAO Zhao-yun, ZHU Zhi-wei, SHAO Guo-sheng, WANG Guang-ming
. Cd Toxicity and Accumulation in Rice Plants Vary with Soil Nitrogen Status and Their Genotypic Difference can be Partly Attributed to Nitrogen Uptake Capacity[J]. Rice Science, 2009
, 16(4)
: 283
-291
.
DOI: 10.1016/S1672-6308(08)60092-X
1 Das P, Samantaray S, Rout G R. Studies on cadmium toxicity in plants: A review. Environ Poll, 1997, 89: 29–36.
2 Grant C A, Buckley W T, Bailey L D, Selles F. Cadmium accumulation in crops. Can J Plant Sci, 1998, 78: 1–17.
3 Shao G, Chen M, Wang D, Xu C, Mou R, Cao Z, Zhang X. Using iron fertilizer to control Cd accumulation in rice plants: A new promising technology. Sci in China Series C: Life Sci, 2008, 51(3), 245–253.
4 Nakanishi H, Ogawa I, Ishimaru Y, Mori S, Nishizawa N K. Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+ transporters OsIRT1 and OsIRT2 in rice. Soil Sci Plant Nutri, 2006, 52(4): 464–469.
5 Ramesh S A, Shin R, Eide D J, Schachtman D P. Differential metal selectivity and gene expression of two zinc transporters from rice. Plant Physiol, 2003, 133: 126–134.
6 Isaure M P, Fayard B, Sarret G, Pains S, Bourguignon J. Localization and chemical forms of cadmium in plant samples by combining analytical electron microscopy and X-ray spectromicroscopy. Spectrochimica Acta. Part B: Atomic Spectroscopy, 2006, 61(12): 1242–1252.
7 Weigel H J, Jäger H J. Subcellular distribution and chemical form of cadmium in bean plants. Plant Physiol, 1980, 65: 480–482.
8 Gong J M, Lee D A, Schroeder J I. Long-distance root-to- shoot transport of phytochelatins and cadmium in Arabidopsis. Proc Natl Acad Sci USA, 2003, 100: 10118–10123.
9 Li J C, Guo J B, Xu W Z, Ma M. RNA interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds. J Integ Plant Biol, 2007, 49(7): 1032–1037.
10 Chaffei C, Pageau K, Suzuki A, Gouia H, Ghorbel M H, Masclaux-Daubresse C. Cadmium toxicity induced changes in nitrogen management in Lycopersicon esculentum leading to a metabolic safeguard through an amino acid storage strategy. Plant Cell Physiol, 2004, 45(11): 1681–1693.
11 Chaffei C, Gouia H, Ghorbel H M. Nitrogen metabolism in tomato plants under cadmium stress. J Plant Nutri, 2003, 26(8): 1617–1634.
12 Boussama N, Ouariti O, Suzuki A, Ghorbla M H. Cd-stress on nitrogen assimilation. Plant Physiol, 1999, 155: 310–317.
13 Prasad M N V. Cadmium toxicity and tolerance in vascular plants. Environ Exp Bot, 1995, 35(4): 525–545.
14 Cobbett C S. Phytochelatins and their roles in heavy metal fetoxification. Plant Physiol, 2000, 123: 825–832.
15 Grill E, Winnacker E L, Zenk M H. Phytochelatins: The principal heavy-metal complexing peptides of higher plants. Science, 1985, 230(4726): 674–676.
16 Gasic K, Korban S S. Transgenic Indian mustard Brassica juncea plants expressing an Arabidopsis phytochelatin synthase AtPCS1 exhibit enhanced As and Cd tolerance. Plant Mol Biol, 2007, 64: 361–369.
17 Lee S, Moon J S, Ko T S, Petros D, Goldsbrough P B, Korban S S. Overexpression of Arabidopsis phytochelatin synthase paradoxically leads to hypersensitivity to cadmium stress. Plant Physiol, 2003, 131: 656–663.
18 Finkemeier I, Kluge C, Metwally A, Georgi M, Grotjohann N, Dietz K J. Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare. Plant, Cell & Environ, 2003, 26: 821–833.
19 Vlek P L G, Bymes B H. The efficiency and loss of fertilizer N in lowland rice. Fertilizer Res, 2000, 9: 131–147.
20 Cassamn K G, Kropf M J, Gaunt J. Nitrogen use efficiency of rice reconsidered: What are the key constraints? Plant & Soil, 1993, 155/156: 359–362.
21 Chaturvedi I. Effect of nitrogen fertilizers on growth, yield and quality of hybrid rice (Oryza sativa L.). J Cent Eur Agric, 2005, 6(4): 611–618.
22 Hassan M J, Zhang G P, Zhu Z J. Influence of cadmium toxicity on plant growth and nitrogen uptake in rice as affected by nitrogen form. J Plant Nutri, 2008, 31(2): 251– 262.
23 Hassan M J, Wang F, Shaukat A, Zhang G P. Toxic effect of cadmium on rice as affected by nitrogen fertilizer form. Plant & Soil, 2005, 277(1/2): 359–365.
24 Jalloha M A, Chen J H, Zhen F R, Zhang G P. Effect of different N fertilizer forms on antioxidant capacity and grain yield of rice growing under Cd stress. J Hazard Mater, 2008, 162(2/3): 1081–1085.
25 Tang Q Y, Feng M G. Practical Statistics and Its Data Processing System Statistical Software Package. Beijing: China Agricultural Press, 1997. (in Chinese)
