Research Paper

Diversity Analysis in Selected Non-basmati Scented Rice Collection

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  • 1)Department of Botany, University of Pune, Pune-411007, India; 2)Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai-400085, India

Received date: 2009-10-04

  Online published: 2010-03-28

Supported by

the University of Pune-Bhabha Atomic Research Centre collaborative research programme, financial assistance by Department of Science and Technology (DST), New Delhi and University of Pune, Pune India.

Abstract

Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) marker systems. The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism, respectively. The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87. RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions. However, the analysis using a combined data could group basmati and non-basmati scented rice accessions separately. The bands present specifically among three accessions of non-basmati scented rice were also identified. The study revealed a high genetic diversity among non-basmati scented rice accessions.

Cite this article

Sarika MATHURE, Narendra JAWALI, Altafhusain NADAF . Diversity Analysis in Selected Non-basmati Scented Rice Collection[J]. Rice Science, 2010 , 17(1) : 35 -42 . DOI: 10.1016/S1672-6308(08)60102-X

References

1 National Bureau of Plant Genetic Resources (NBPGR). Perspective Plan Vision 2025. New Delhi, India: NBPGR, 2007: 9–24.
2 Singh R K, Singh U S, Khush G S, Rohilla R, Singh J P, Singh G, Shekhar K S. Small and medium grained aromatic rices of India. In: Singh R K, Singh U S, Khush G S. Aromatic Rices. New Delhi: Oxford & IBH Publication, 2000: 155–177.
3 Bhuyan N, Borah B K, Sarma R N. Genetic diversity analysis in traditional lowland rice (Oryza sativa L.) of Assam using RAPD and ISSR markers. Curr Sci, 2007, 93(7): 967–972.
4 Joshi R K, Behera L. Identification and differentiation of indigenous non-basmati aromatic rice genotypes of India using microsatellite markers. Afr J Biotech, 2006, 6(4): 348–354.
5 Singh D, Sirohi A, Vikas, Sharma S, Kumar V, Gaur A, Chaudhari R, Chaudhari N. RAPD based identification of farmer’s collections of Kalanamak and traditional varieties of basmati rice. Ind J Crop Sci, 2006, 1(1-2): 102–105.
6 Davierwala A P, Chawdari K V, Kumar S, Reddy A P K, Ranjekar P K, Gupta V S. Use of three different marker systems to estimate genetic diversity of Indian elite rice varieties. Genetica, 2000, 108: 269–284.
7 Hari Prasad A S, Neeraja C N, Sarla N, Sadiq E A. Comparative evaluation of SSR and ISSR markers for polymorphism in landraces and elite varieties of rice (Oryza sativa L.). J Plant Biochem Biol, 2005, 14: 49–51.
8 Nagaraju J, Kathirvel M, Kumar R R, Siddiq E A, Hasnain S E. Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers. Proc Natl Acad Sci USA, 2002, 99: 5836–5841.
9 Qian W, Ge S, Hong D Y. Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theor Appl Genet, 2001, 102: 440–499.
10 Saker M M, Youssef S S, Abdallah N A, Bashandy H S, El-Sharkawy A M. Genetic analysis of some Egyptian rice genotypes using RAPD, SSR and AFLP. Afr J Biotech, 2005, 4(9): 882–890.
11 Nalini E, Bhagwat S G, Jawali N. A simple method for isolation of DNA from plants suitable for long term storage and DNA marker analysis. BARC News Lett, 2004, 249: 208–214.
12 Prasad S, Reddy K S, Jawali N. Abundance of random amplified hybridizing microsatellites in mungbean (Vigna radiata L. Wilzeck). Asia Pac J Mol Biol & Biotech, 1999, 7: 173–177.
13 Van de Peer Y, De Wachter R. TREECON: A software package for the construction and drawing of evolutionary trees. Comput Appl Biosci, 1993, 9: 177–182.
14 Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 1979, 76: 5269–5273.
15 Nerkar Y S, Patil V D, Misal M B, Katare N B. Aromatic rices of Maharashtra. In: Singh R K, Singh U S. A treatise on the scented rices of India. New Delhi: Kalyani Publishers, 2003: 339–346.
16 Raychaudhari P, Kohli S, Srinivasan K, Mohapatra T, Sharma R P. Identification and classification of aromatic rices based on DNA fingerprinting. Euphytica, 2001, 118: 243–251.
17 Verma S K, Khanna V, Singh N. Random amplified polymorphic DNA analysis of Indian scented basmati rice (Oryza sativa L.) germplasm for identification of variability and duplicate accessions, if any. Electrophoresis, 1999, 20: 1786–1789.
18 Raghunathachari P, Khanna V K, Singh U S, Singh N K. RAPD analysis of genetic variability in Indian scented rice germplasm (Oryza sativa L.). Curr Sci, 2000, 79(7): 994–998.
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