Research Paper

Dissection of QTLs for Hull Silicon Content on the Short Arm of Rice Chromosome 6

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  • Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received date: 2009-12-23

  Online published: 2010-06-28

Supported by

the National Natural Science Foundation of China (Grant No. 30571062), National Hi-Tech Research and Development Program of China (Grant No. 2006AA 10Z1E8) and the Program of Super Rice from Chinese Agricultural Ministry (Grant No. 200906).

Abstract

The QTL qHUS6 for hull silicon content in rice was previously located on the short arm of rice chromosome 6. By using an F2:3 population segregating in the RM587–RM19784 region harboring qHUS6 in an isogenic background, two QTLs for hull silicon content were detected, of which qHUS6-1 was located in the distal region and qHUS6-2 in the region proximal to the centromere. Three rice plants carrying small heterozygous segments in the target region were selected, of which two covered the qHUS6-1 region and the other covered the qHUS6-2 region. Three F2:3 populations were derived from the selfed seeds of the three plants, respectively. QTL mapping was performed using the two populations segregating in the qHUS6-1 region, and qHUS6-1 was delimited to a 147.0-kb region flanked by the markers RM510 and RM19417. Five groups of F3 lines with different genotypic compositions in the qHUS6-2 region were selected from the other F2:3 population. Two QTLs were separated with two-way ANOVA, of which qHUS6-2a was located in the interval defined by RM19706–RM19795 and qHUS6-2b in the interval RM314–RM19665.

Cite this article

WU Ji-rong, FAN Fang-jun, DU Jing-hong, FAN Ye-yang, ZHUANG Jie-yun . Dissection of QTLs for Hull Silicon Content on the Short Arm of Rice Chromosome 6[J]. Rice Science, 2010 , 17(2) : 99 -104 . DOI: 10.1016/S1672-6308(08)60111-0

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