Rice with low glutelin content is suitable as functional food for patients affected by kidney failure. Low glutelin- content gene Lgc1 in rice has a 3.5-kb deletion between two highly similar glutelin genes GluB4 and GluB5, which locates on the short arm of chromosome 2. To improve the selection efficiency in low glutelin-content rice breeding, two molecular markers designated as InDel-Lgc1-1 and InDel-Lgc1-2 were developed to detect the low glutelin-content gene Lgc1. A double PCR detection indicated that combined use of the two markers could easily distinguish the genotypes of Lgc1 from different rice varieties. Therefore, as a simple and low-cost technique, the molecular marker could be widely used to identify different varieties with Lgc1 gene and applied in marker-assisted selection of low glutelin-content rice.
CHEN Tao, TIAN Meng-xiang, ZHANG Ya-dong, ZHU Zhen, ZHAO Ling, ZHAO Qing-yong, LIN Jing, ZHOU Li-hui, WANG Cai-lin
. Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)[J]. Rice Science, 2010
, 17(3)
: 173
-178
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DOI: 10.1016/S1672-6308(09)60014-7
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