A thermo-sensitive white stripe-leaf mutant (tws) was selected from the M2 progeny of a japonica variety, Jiahua 1, treated by 60Co γ-radiation. In comparison with the wild type parent, the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn normal green on the 5th leaf when grown at low temperatures (20°C and 24°C). Furthermore, the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermo-sensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws. In addition, by using SSR markers and an F2 segregating population derived from the cross between the tws mutant and 9311, tws was mapped between the markers MM3907 and MM3928 with a physical distance of 86 kb on rice chromosome 4
LI Chao, LIN Dong-zhi, DONG Yan-jun, YE Sheng-hai, ZHANG Xiao-ming
. Genetic Analysis and Mapping of a Thermo-sensitive White Stripe-Leaf Mutant at Seedling Stage in Rice (Oryza sativa)[J]. Rice Science, 2010
, 17(4)
: 276
-281
.
DOI: 10.1016/S1672-6308(09)60027-5
Alberte R S, Hesketh J D, Hofstra G, Thornber J P, Naylor A W, Bernard R L, Brim C, Endrizzi J, Kohel R J. 1974. Composition and activity of the photosynthetic apparatus in temperature sensitive mutants of higher plants. Proc Natl Acad Sci USA, 71: 2414–2418.
Cui H R, Xia Y W, Gao M W. 2001. The effects of temperature on leaf color and chlorophyll biosynthesis of rice mutant W1. Acta Agric Nucl Sin, 15(5): 269–273. (in Chinese with English abstract)
Dong W Q, Dong Y J, Shi S J. 1995. Inheritance and fertility research of the thermo-sensitive albino seedling phenotype in double-functional genic male-sterile lines 7436S. China Seeds, (1): 19–21. (in Chinese)
Dong Y J, Shi S Y, Zhang H D, Chen S H, Sun Z X. 1994. Temperature response and inheritance of a thermo-sensitive seedling colour mutant in rice (Oryza sativa L.). J Genet & Breeding, 48: 423–426.
Dong Y J, Dong W Q, Zang X M, Shi S Y, Zhang H D. 1995. The genetic analysis of a thermo-sensitive seedling-colour mutant Fan5. Chin J Rice Sci, 9(4): 249–250. (in Chinese with English abstract)
Dong Y J, Dong W Q, Shi S Y, Jin Q S. 2001. Identification and genetic analysis of a thermo-sensitive seedling-colour mutant in rice (Oryza sativa L.). Breeding Sci, 51: 1–4.
Dwyer L M, Tollenaar M, Houwing L. 1991. A nondestructive method to monitor leaf greenness in corn. Can J Plant Sci, 71: 505–509.
Gálová E, Böhmová B, Sevcovicová A. 2000. Analysis of some barley chlorophyll mutants and their response to temperature stress. Photosynthetica, 38: 29–35.
Han B, Xue Y B, Li J Y, Deng X W, Zhang Q F. 2007. Rice functional genomics research in China. Phil Trans R Soc B, 362(1482): 1009–1021.
Hiroki S, Kensuke K, Ko N, Masahiro Y, Atsushi Y, Koh I. 2007. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 52: 512–527.
Kusumi K, Mizutani A, Nishimura M, Iba K. 1997. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 12(6): 1241–1250.Kurata N, Miyoshi K, Nonomura K, Ken I N, Yamazaki Y, Yukihiro I. 2005. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 46: 48–62.
Li N, Chu H W, Wen T Q, Zhang D B. 2007. Genetic analysis and mapping of the rice white midrib mutant Oswm. Acta Agric Shanghai, 23(1): 1–4. (in Chinese with English abstract)
Lincoln S E, Daly M J, Lander E S. 1993. Constructing genetics maps with MAPMAKER/EXP 3.0: A tutorial and reference manual. In: A Whitehead Institute for Biomedical Research Technical Report. 3rd edn. Cambridge MA: Whitehead Institute.
Liu W Z, Fu Y P, Hu G C, Si H M, Zhu L, Wu C, Sun Z X. 2007. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta, 226: 785–795.
Markwell J, Osterman J C. 1992. Occurrence of temperature- sensitive phenotypic plasticity in chlorophyll-deficient mutants of Arabidopsis thaliana. Plant Physiol, 98: 392–394.
Muray M G, Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 8(19): 4321–4325.
Pasini L, Bruschini S, Bertoli A, Mazza R, Fracheboud Y, Marocco A. 2005. Photosynthetic performance of cold-sensitive mutants of maize at low temperature. Physiol Plant, 124: 362–370.
Shu Q Y, Liu G F, Xia Y W. 1996. The research of leaf color thermo- sensitive mutant. Acta Agric Nucl Sin, 10(1): 6–10. (in Chinese with English abstract)
Soo C Y, Sung H C, Hiroki S, Li J J, Kensuke K, Hee J K, Koh I, Nam C P. 2009. Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 150: 388–401.
Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, Iba K. 2004. The virescent 2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 45(8): 985–996.
Watanabe S, Hatanaka Y, Inada K. 1980. Development of a digital chlorophyll meter: I. Structure and performance. Jpn J Crop Sci, 49(Special issue): 89–90.
Xia J C, Wang Y P, Ma B T, Yin Z Q, Hao M, Kong D W, Li S G. 2006. Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.). J Genet Genom, 33(12): 1112–1119.
