Experimental Technique

A Simple Method for Preparation of Rice Genomic DNA

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  • 1)State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2)Agricultural College, Yangzhou University, Yangzhou 225009, China; #These authors contributed equally to this paper

Received date: 2010-07-22

  Revised date: 1900-01-01

  Online published: 2010-12-28

Abstract

DNA extraction; high-throughput PCR; marker assisted selection; gene mapping; rice

Cite this article

SUN Chuan, HE Ying-hong, CHEN Gang, RAO Yu-chun, ZHANG Guang-heng, GAO Zhen-yu, LIU Jian, JU Pei-na, HU Jiang, GUO Long-biao, QIAN Qian, ZENG Da-li . A Simple Method for Preparation of Rice Genomic DNA[J]. Rice Science, 2010 , 17(4) : 326 -329 . DOI: 10.1016/S1672-6308(09)60034-2

References

Aljanabi S M, Martinez I. 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR based techniques. Nucl Acids Res, 25: 4692–4693.

Ausubel F M, Brent R, Kingston R E. 1996. Phenol/SDS method for plant RNA preparation. In: Current Protocols in Molecular Biology. Vol.1. New York: John Wiley and Sons, Inc.

Chen W Y, Cui H R, Bao J S, Zhou X S, Shu Q Y. 2006. A simplified rice DNA extraction protocol for PCR analysis. Rice Sci, 13(1): 67–70.

Dilworth E, Frey J E. 2000. A rapid method for high throughput DNA extraction from plant material for PCR amplification. Plant Mol Biol Rep, 18: 61–64.

John M E. 1992. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucl Acids Res, 20(9): 2381.

Kamalay J, Tejwani R, Keith R G. 1990. Isolation and analysis of genomic DNA from single seeds. Crop Sci, 30(5): 1079–1084.

Mace E S, Buhariwalla H K, Crouch J H. 2003. A high-throughput DNA extraction protocol for tropical molecular breeding programs. Plant Mol Biol Rep, 21(4): 459a–459h.

McCouch S R, Kochert G, Yu Z H, Wang Z Y, Khush G S, Coffman W R, Tanksley S D. 1988. Molecular mapping of rice chromosomes. Theor Appl Genet, 76: 815–829.

Paris M, Carter M. 2000. Cereal DNA: A rapid high-throughput extraction method for marker-assisted selection. Plant Mol Biol Rep, 18(4): 357–360.

Qiu F L, Wang H H, Chen J, Zhuang J Y, Heung H, Cheng S H, Wu J L. 2006. A rapid DNA mini-prep method for large-scale rice mutant screening. Rice Sci, 13(4): 299–302.

Sambrook J, Russell D. 2002. Molecular Cloning: A Laboratory Manual. 3rd edn. translated by Huang P T. Beijing: Science Press. (in Chinese)

Silvy M, Pic G, Gabert J, Picard C. 2004. Improvement of gene expression analysis by RQ-PCR technology: Addition of BSA. Leukemia, 18(5): 1022–1025.

Steenkamp J, Wild I, Lourens A, Helden V P. 1991. Improved method for DNA extraction from Vitis vinifera. Am J Enol Vit, 45: 102–106.

Stewart C N Jr, Via L E. 1993. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechnique, 14(5): 748–750.

Wang H W, Wu C, Zhao X Y, Wang J H, Zhang R Y, Wang Y F. 2002. A rapid method for preparation of PCR template using SDS. J Hebei Univ: Nat Sci, 22(2): 186–188. (in Chinese with English abstract)

Wang L, Long Y M, Liu Y G. 2009. A method for rapid preparation of plant genomic DNA for PCR analysis. Mol Plant Breeding, 7(2): 425–428. (in Chinese with English abstract)

Wang X F, Yang J B, Xiang T H, Li L, Ni D H. 2002. A new method for PCR reaction from alkali-treated rice leaf tissues. Chin J Rice Sci, 16(1): 67–70. (in Chinese with English abstract)

Yu S W, Liu Y B. 2003. Application of molecular marker techniques in rice genetics and breeding. Acta Agric Univ Jiangxi, 25(1): 111–116. (in Chinese with English abstract)

Zhao H X, Xie P, Huang Z J, Ju C M, Zhou Y. 2006. An improved method of extracting rice genomic DNA. J Hubei Univ: Nat Sci, 12(4): 389–392. (in Chinese with English abstract)
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