Purification and Identification of Glutathione S-transferase in Rice Root under Cadmium Stress

ZHANG Chun-hua1, 2, WU Ze-ying3, JU Ting3, GE Ying2, 3

doi:10.1016/S1672-6308(13)60114-6

Rice Science >

2013 , Vol. 20 >Issue 3: 173 - 178

DOI: https://doi.org/10.1016/S1672-6308(13)60114-6

Research Paper

Purification and Identification of Glutathione S-transferase in Rice Root under Cadmium Stress

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1Demonstration Laboratory of Proteomic Research, Laboratory Centre of Life Science, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 3College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Revised date: 2012-12-31

Online published: 2013-03-27

Supported by

Financial supports from the National Natural Science Foundation of China (Grant No. 30700479), Research Fund for the Doctoral Program of Higher Education of China (Grant Nos. 20090097110035 and 20110097110004), Research Fund of State Key Laboratory of Soil and Sustainable Agriculture, Nanjing Institute of Soil Science, Chinese Academy of Science, China (Grant No. Y052010019) are greatly acknowledged.

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Abstract

Cadmium (Cd) contamination in paddy soils poses a serious threat to the production and quality of rice. Among various biochemical processes related to Cd detoxification in rice, glutathione S-transferase (GST) plays an important role, catalyzing Cd complexation with glutathione (GSH) and scavenging reactive oxygen species (ROS) in cells. In this study, a hydroponic experiment was conducted to investigate the response of GST isozymes in rice roots upon Cd exposure. Results showed that the GST activity in rice roots was clearly enhanced by 50 μmol/L Cd treatment for 7 d. The GST isozymes were purified by ammonium sulphate precipitation, gel filtration chromatography and affinity chromatography. After being separated by SDS-PAGE and visualized by silver staining, GSTU6 was identified by in-gel digestion, MALDI-TOF-MS analysis and peptide mass fingerprint. The results confirm the vital function of tau class rice GST in Cd detoxification.

Key words： rice; glutathione S-transferase, cadmium stress; purification; identification; isozyme

Cite this article

ZHANG Chun-hua1, 2, WU Ze-ying3, JU Ting3, GE Ying2, 3 . Purification and Identification of Glutathione S-transferase in Rice Root under Cadmium Stress[J]. Rice Science, 2013 , 20(3) : 173 -178 . DOI: 10.1016/S1672-6308(13)60114-6

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