Confirmation of Novel Quantitative Trait Loci for Seed Dormancy at Different Ripening Stages in Rice

Kazuhiro SASAKI1, 2, Yuri KAZAMA1, Youn CHAE1, Tadashi SATO1

doi:10.1016/S1672-6308(13)60123-7

Rice Science >

2013 , Vol. 20 >Issue 3: 207 - 212

DOI: https://doi.org/10.1016/S1672-6308(13)60123-7

Research Paper

Confirmation of Novel Quantitative Trait Loci for Seed Dormancy at Different Ripening Stages in Rice

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1Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan; *Present address: Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

Revised date: 2012-12-31

Online published: 2013-03-27

Supported by

This work was supported in part by a Grant-in-Aid from the Ministry of Agriculture, Forestry and Fisheries, Japan (Genomics for Agricultural Innovation, QTL-4009).

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Abstract

Seed dormancy contributes resistance to pre-harvest sprouting. Effects on respective quantitative trait loci (QTLs) for dormancy should be assessed by using fresh seeds before germinability altered through storage. We investigated QTLs related to seed dormancy using backcross inbred lines derived from a cross between Nipponbare and Kasalath. Four putative QTLs for seed dormancy were detected immediately after harvest using composite interval mapping. These putative QTLs were mapped near C1488 on chromosome 3 (qSD-3.1), R2171 on chromosome 6 (qSD-6.1), R1245 on chromosome 7 (qSD-7.1) and C488 on chromosome 10 (qSD-10.1). Kasalath alleles promoted dormancy for qSD-3.1, qSD-6.1 and qSD-7.1, and the respective proportions of phenotypic variation explained by each QTL were 12.9%, 9.3% and 8.1%. We evaluated the seed dormancy harvested at different ripening stages during seed development using chromosome segment substitution lines (CSSLs) to confirm gene effects. The germination rates of CSSL27 and CSSL28 substituted with the region including qSD-6.1 were significantly lower than those of Nipponbare and other CSSLs at the late ripening stage. Therefore, qSD-6.1 is considered the most effective novel QTL for pre-harvest sprouting resistance among the QTLs detected in this study.

Key words： chromosome segment substitution line; heading date; quantitative trait locus; rice; seed dormancy

Cite this article

Kazuhiro SASAKI1, 2, Yuri KAZAMA1, Youn CHAE1, Tadashi SATO1 . Confirmation of Novel Quantitative Trait Loci for Seed Dormancy at Different Ripening Stages in Rice[J]. Rice Science, 2013 , 20(3) : 207 -212 . DOI: 10.1016/S1672-6308(13)60123-7

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