Phytochromes are Involved in Elongation of Seminal Roots and Accumulation of Dry Substances in Rice Seedlings

ZHENG Jun1, 2, ZHOU Jin-jun2, 3, ZHAO Jie2, 3, ZHAO Shu-zhen2, 3, LI Guo-rong1, XIE Xian-zhi2, 3

doi:10.1016/S1672-6308(13)60115-8

Rice Science >

2013 , Vol. 20 >Issue 2: 88 - 94

DOI: https://doi.org/10.1016/S1672-6308(13)60115-8

Research Paper

Phytochromes are Involved in Elongation of Seminal Roots and Accumulation of Dry Substances in Rice Seedlings

Expand

1College of Life Sciences, Shandong Normal University, Jinan 250014, China; 2High-Tech Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, China

Online published: 2013-01-30

Supported by

This work was partly supported by grants from the Shandong Natural Science Funds for Distinguished Young Scholar, China (Grant No. JQ200911), the Chinese National Natural Science Foundation (Grant No. 30971744), and the Chinese Ministry of Agriculture (Grant No. 2009ZX08001-029B).

Fold

Abstract

Phytochromes have been reported to play important roles in seedling de-etiolation and flowering in rice. To identify the roles of phytochromes in regulating root growth and accumulation of dry substances, the lengths of seminal roots and the dry weights of seedlings were measured in the wild type as well as the phytochrome A (phyA) and phytochrome B (phyB) mutants grown under different conditions. When the whole seedlings were exposed to white light, the elongation of the seminal roots was significantly photoinhibited in the wild type, whereas this inhibitory effect was clearly reduced in the phyA and phyB mutants. When the roots of the seedlings were blocked from white light, the phyA and phyB mutants exhibited significantly longer seminal roots than the wild type. These results suggest that both the root-localized and shoot-localized PHYA and PHYB are involved in the photoinhibition of seminal root elongation in rice seedlings. By measuring the dry weights of roots and shoots, it is revealed that PHYB positively regulates the accumulation of dry substances in shoots, however, PHYA exerts the contrary effects on the accumulation of dry substances in roots and shoots of rice seedlings.

Key words： rice; phytochrome; seminal root; dry substance

Cite this article

ZHENG Jun1, 2, ZHOU Jin-jun2, 3, ZHAO Jie2, 3, ZHAO Shu-zhen2, 3, LI Guo-rong1, XIE Xian-zhi2, 3 . Phytochromes are Involved in Elongation of Seminal Roots and Accumulation of Dry Substances in Rice Seedlings[J]. Rice Science, 2013 , 20(2) : 88 -94 . DOI: 10.1016/S1672-6308(13)60115-8

References

Basu D, Dehesh K, Schneider-Poetsch H J, Harrington S E, McCouch S R, Quail P H. 2000. Rice PHYC gene: Structure, expression, map position and evolution. Plant Mol Biol, 44: 27–42.
Briggs W R, Huala E. 1999. Blue-light photoreceptors in higher plants. Annu Rev Cell Dev Biol, 15: 33–62.
Chen H, Zhang J, Neff M M, Hong S W, Zhang H, Deng X W, Xiong L. 2008. Integration of light and abscisic acid signaling during seed germination and early seedling development. Proc Natl Acad Sci USA, 105: 4495–4500.
Correll M J, Kiss J Z. 2005. The roles of phytochromes in elongation and gravitropism of roots. Plant Cell Physiol, 46: 317–323.
De Simone S, Oka Y, Inoue Y. 2000. Photoperceptive site of the photoinduction of root hairs in lettuce (Lactuca sativa L. cv. Grand Rapids) seedlings under low pH conditions. J Plant Res, 113: 55–62.
Dehesh K, Tepperman J, Christensen A H, Quail P H. 1991. phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol Gen Genet, 225: 305–313.
Devlin P F, Yanovsky M J, Kay S A. 2003. A genomic analysis of the shade avoidance response in Arabidopsis. Plant Physiol, 133: 1617–1629.
Franklin K A, Quail P H. 2010. Phytochrome functions in Arabidopsis development. J Exp Bot, 61: 11–24.
Iwamoto M, Kiyota S, Hanada A, Yamaguchi S, Takano M. 2011. The multiple contributions of phytochromes to the control of internode elongation in rice. Plant Physiol, 157: 1187–1195.
Jumtee K, Okazawa A, Harada K, Fukusaki E, Takano M, Kobayashi A. 2009. Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves. J Biosci Bioeng, 108: 151–159.
Kay S A, Keith B, Shinozaki K, Chua N H. 1989. The sequence of the rice phytochrome gene. Nucl Acids Res, 17: 2865–2866.
Kiss J Z, Miller K M, Ogden L A, Roth K K. 2002. Phototropism and gravitropism in lateral roots of Arabidopsis. Plant Cell Physiol, 43: 35–43.
Kurata T, Yamamoto K T. 1997. Light-stimulated root elongation in Arabidopsis thaliana. J Plant Physiol, 151: 346–351.
Lin R C, Wang H Y. 2005. Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport. Plant Physiol, 138: 949–964.
Liu J, Liu Y M, Takano M, Wang B, Xie X Z. 2010. Involvement of phytochromes in gibberellin-mediated photomorphogenesis in rice seedlings. Chin Sci Bull, 55: 2384–2390. (in Chinese)
Molas M L, Kiss J Z, Correll M J. 2006. Gene profiling of the red light signalling pathways in roots. J Exp Bot, 57: 3217–3229.
Nagatani A, Reed J W, Chory J. 1993. Isolation and initial characterization of Arabidopsis mutants that are deficient in phytochrome A. Plant Physiol, 102: 269–277.
Nagatani A. 2004. Light-regulated nuclear localization of phytochromes. Curr Opin Plant Biol, 7: 708–711.
Neff M M, Fankhauser C, Chory J. 2000. Light: An indicator of time and place. Genes Dev, 14: 257–271.
Oh E, Kang H, Yamaguchi S, Park J, Lee D, Kamiya Y, Choi G. 2009. Genome-wide analysis of genes targeted by PHYTOCHROME INTERACTING FACTOR 3-LIKE5 during seed germination in Arabidopsis. Plant Cell, 21: 403–419.
Ohno Y, Fujiwara A. 1967. Photoinhibition of elongation of roots in rice seedlings. Plant Cell Physiol, 8: 141–150.
Okada K, Shimura Y. 1992. Mutational analysis of root gravitropism and phototropism of Arabidopsis thaliana seedlings. Funct Plant Biol, 19: 439–448.
Osugi A, Itoh H, Ikeda-Kawakatsu K, Takano M, Izawa T. 2011. Molecular dissection of the roles of phytochrome in photoperiodic flowering in rice. Plant Physiol, 157: 1128–1137.
Riemann M, Muller A, Korte A, Furuya M, Weiler E W, Nick P. 2003. Impaired induction of the jasmonate pathway in the rice mutant hebiba. Plant Physiol, 133(4): 1820–1830.
Saab I N, Sharp R E, Pritchard J, Voetberg G S. 1990. Increased endogenous abscisic acid maintains primary root growth and inhibits shoot growth of maize seedlings at low water potentials. Plant Physiol, 93: 1329–1336.
Salisbury F J, Hall A, Grierson C S, Halliday K J. 2007. Phytochrome coordinates Arabidopsis shoot and root development. Plant J, 50: 429–438.
Seo M, Hanada A, Kuwahara A, Endo A, Okamoto M, Yamauchi Y, North H, Marion-Poll A, Sun T P, Koshiba T, Kamiya Y, Yamaguchi S, Nambara E. 2006. Regulation of hormone metabolism in Arabidopsis seeds: Phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism. Plant J, 48: 354–366.
Shimizu H, Tanabata T, Xie X Z, Inagaki N, Takano M, Shinomura T, Yamamoto K T. 2009. Phytochrome-mediated growth inhibition of seminal roots in rice seedlings. Physiol Plant, 137: 289–297.
Shimizu H, Shinomura T, Yamamoto K T. 2010. Similarities and differences between phytochrome-mediated growth inhibition of coleoptiles and seminal roots in rice seedlings. Plant Signal Behav, 5(2): 134–135.
Sidler M, Hassa P, Hasan S, Ringli C, Dudler R. 1998. Involvement of an ABC transporter in a developmental pathway regulating hypocotyl cell elongation in the light. Plant Cell, 10: 1623– 1636.
Sun Q, Yoda K, Suzuki M, Suzuki H. 2003. Vascular tissue in the stem and roots of woody plants can conduct light. J Exp Bot, 54: 1627–1635.
Svyatyna K, Riemann M. 2012. Light-dependent regulation of the jasmonate pathway. Protoplasma, 249(Suppl. 2): 137–145.
Takano M, Kanegae H, Shinomura T, Miyao A, Hirochika H, Furuya M. 2001. Isolation and characterization of rice phytochrome A mutants. Plant Cell, 13: 521–534.
Takano M, Inagaki N, Xie X Z, Yuzurihara N, Hihara F, Ishizuka T, Yano M, Nishimura M, Miyao A, Hirochika H, Shinomura T. 2005. Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice. Plant Cell, 17: 3311–3325.
Takano M, Inagaki N, Xie X Z, Kiyota S, Baba-Kasai A, Tanabata T, Shinomura T. 2009. Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice. Proc Natl Acad Sci USA, 106: 14705–14710.
Tian Q, Uhlir N J, Reed J W. 2002. Arabidopsis SHY2/IAA3 inhibits auxin-regulated gene expression. Plant Cell, 14: 301–319.
Xie X Z, Shinomura T, Inagaki N, Kiyota S, Takano M. 2007. Phytochrome-mediated inhibition of coleoptile growth in rice: Age-dependency and action spectra. Photochem Photobiol, 83: 131–138.
Xie X Z, Xue Y J, Zhou J J, Zhang B, Chang H, Takano M. 2011. Phytochromes regulate SA and JA signaling pathways in rice and are required for developmentally controlled resistance to Magnaporthe grisea. Mol Plant, 4(4): 688–696.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References