Morphological and Anatomical Assessment of KDML 105 (Oryza sativa L. spp. indica) and Its Mutants Induced by Low-Energy Ion Beam

Narumol BOONRUENG, Somboon ANUNTALABHOCHAI, Arunothai JAMPEETONG

doi:10.1016/S1672-6308(13)60129-8

2013 , Vol. 20 >Issue 3: 213 - 219

DOI: https://doi.org/10.1016/S1672-6308(13)60129-8

Research Paper

Morphological and Anatomical Assessment of KDML 105 (Oryza sativa L. spp. indica) and Its Mutants Induced by Low-Energy Ion Beam

Expand

Department of Biology, Faculty of Science, Chiang Mai University, Meuang, Chiang Mai 50202, Thailand

Online published: 2013-03-27

Fold

Abstract

Thai jasmine rice KDML 105 is consumed around the world. BKOS, PKOS and TKOS are new cultivars produced from low-energy ion beam induction in KDML 105. The purpose of this study is to compare the morphology and anatomy between KDML 105 and the three new cultivars. Seeds of the four cultivars were germinated and grown in pots until flowering phase. The plants’ organs were observed and the lengths of culms, ligules, leaves and panicles were measured. Leaf surface area was calculated and numbers of roots, spikelets and tillers were counted. BKOS and PKOS had significantly shorter culms than KDML 105 and TKOS. The largest leaf area was found in KDML 105 followed by TKOS, BKOS and PKOS, respectively. Numbers of roots and tillers in BKOS and TKOS were significantly fewer than those in KDML 105 and PKOS. The number of spikelets per plant in BKOS was the lowest among all cultivars. For anatomical comparison, cross sections of culms and roots were observed. All plants had a similar arrangement of tissues, but the number and size of cells were different. Furthermore, longitudinal sections of culms showed that the lengths of epidermal and parenchyma cells were directly related with the length of the culm. To compare the leaves, both stomata and epidermal cells were counted and the lengths of the guard cells were measured. The lengths of guard cells of BKOS and PKOS were shorter, but the stomatal density and the stomatal index were significantly greater than those of KDML 105. For TKOS, though the length of guard cells was shorter than that in KDML 105, the difference was not significant. However, the stomatal density and stomatal index were significantly higher than those in KDML 105.

Key words： jasmine rice; nitrogen ion implantation; mutants; trait; stomatal index

Cite this article

Narumol BOONRUENG, Somboon ANUNTALABHOCHAI, Arunothai JAMPEETONG . Morphological and Anatomical Assessment of KDML 105 (Oryza sativa L. spp. indica) and Its Mutants Induced by Low-Energy Ion Beam[J]. Rice Science, 2013 , 20(3) : 213 -219 . DOI: 10.1016/S1672-6308(13)60129-8

References

Krasaechai A, Yu L D, Sirisawad T, Phornsawatchai T, Bundithya W, Taya U, Anuntalabhochai S, Vilaithong T. 2009. Low-energy ion beam modification of horticultural plants for induction mutation. Surf Coat Technol, 203: 2525–2530.
Laza M R C, Kondo M, Ideta O, Barlaan E, Imbe T. 2010. Quantitative trait loci for stomatal density and size in lowland rice. Euphytica, 172: 149–158.
Maiti R, Rodriguez H G. 2010. Plant architecture determines the productivity potential of a crop: A biochemical genetics tool. IJBSM, 1: 1–3.
Maluszynski M. 2001. Officially released mutant varieties: The FAO/IAEA database. Plant Cell Tiss Org, 65: 175–177.
Muller H J. 1927. Artificial transmutation of the gene. Science, 66: 84–87.
Phanchaisri B, Chandet R, Yu L D, Vilaithong T, Jamjod S, Anuntalabhochai S. 2007. Low-energy ion beam mutation in Thai jasmine rice (Oryza sativa L. cv. KDML 105). Surf Coat Technol, 201: 8024–8028.
Phanchaisri B, Samsang N, Yu L D, Singkarat S, Anuntalabhochai S. 2012. Expression of OsSPY and 14-3-3 genes involved in plant height variations of ion-beam-induced KDML 105 rice mutants. Mutant Res, 734: 56–61.
Semsang N, Kawaree R, Cutler R W, Chundet R, Yu L D, Anuntalabhochai S. 2012. Improved antioxidant activity of BKOS Thai jasmine rice. Nat Prod Res, 26(12): 1–7.
Souza T C, Castro E M, Pereira F J, Parentoni S N, Magalhaes P C. 2009. Morpho-anatomical characterization of root in recurrent selection cycles for flood tolerance of maize (Zea mays L.). Plant Soil Environ, 55: 504–510.
Wang J, Zhu J M, Lin Q Q, Li X J, Teng N J, Li Z S, Li B, Zhang A M, Lin J X. 2006. Effects of stem structure and cell wall components on bending strength in wheat. Chin Sci Bull, 51: 815–823.
Willmer C, Fricker M. 1996. Stomata: Topics in Plant Functional Biology: 2. London: Chapman & Hall.
Wu L F, Yu Z L. 2001. Radiobiological effects of a low-energy ion beam on wheat. Radiat Environ Biophys, 40: 53–57.
Wu J L, Wu C, Lei C, Baraoidan M, Bordeos A, Madamba M R, Ramos-Pamplona M, Mauleon R, Portugal A, Ulat V J, Bruskiewich R, Wang G, Leach J, Khush G, Leung H. 2005. Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol, 59: 85–97.
Wu L L, Liu Z L, Wang J M, Zhou C Y, Chen K M. 2011. Morphological, anatomical, and physiological characteristics involved in development of the large culm trait in rice. Aust J Crop Sci, 5: 1356–1363.
Xu Z Z, Zhou G S. 2008. Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. J Exp Bot, 59: 3317–3325.
Yang H M, Zhang X Y, Wang G X. 2004. Relationships between stomatal character, photosynthetic character and seed chemical composition in grass pea at different water availabilities. J Agric Sci, 142: 675–681.
Yang X C, Hwa C M. 2008. Genetic modification of plant architecture and variety improvement in rice. Heredity, 101: 396–404.
Yu L D, Anuntalabhochai S. 2009. Low-energy ion beam biotechnology at Chiang Mai University. Cambridge: MRS Proceeding, Cambridge University Press.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References